Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F69 I0 R1
|
1426 |
148.0 |
5875178 |
97.4% |
5722423 |
123.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,410,034 |
G→A |
21.5% |
intergenic (+28/‑99) |
rlmB → / → yjfI |
23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,410,034 | 0 | G | A | 21.5%
| 78.3
/ 15.4
| 37 | intergenic (+28/‑99) | rlmB/yjfI | 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein |
| Reads supporting (aligned to +/- strand): ref base G (13/16); new base A (4/4); total (17/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTA > NC_000913/4409893‑4410177
|
cGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGa < 4:865757/149‑1 (MQ=255)
cGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGa < 2:601705/149‑1 (MQ=255)
aTTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATggctt > 3:404148/1‑145 (MQ=255)
aTTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGG‑ctt < 4:404148/148‑1 (MQ=255)
tGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCtata < 1:1109046/149‑1 (MQ=255)
tGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCtata < 3:228828/149‑1 (MQ=255)
tCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCt > 3:1237695/1‑149 (MQ=255)
cAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTg > 1:118898/1‑149 (MQ=255)
cAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGG‑cttt < 1:795905/133‑1 (MQ=255)
cAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCATATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGG‑cttt > 2:795905/1‑133 (MQ=255)
tCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAg > 3:518426/1‑149 (MQ=255)
tttCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaaa < 4:518426/149‑1 (MQ=255)
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt > 2:370910/1‑149 (MQ=255)
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGAGGG‑TTTTTTTTTGTCtata < 1:424042/115‑1 (MQ=255)
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGAGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCtata > 2:424042/1‑115 (MQ=255)
cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTAc > 4:690959/1‑131 (MQ=255)
cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTAc < 3:690959/131‑1 (MQ=255)
tGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt < 4:1237695/149‑1 (MQ=255)
aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGAt > 2:1084944/1‑93 (MQ=255)
aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGAt < 1:1084944/93‑1 (MQ=255)
aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTtta < 1:370910/149‑1 (MQ=255)
gCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa < 2:118898/149‑1 (MQ=255)
ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTc > 2:751018/1‑116 (MQ=255)
ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt > 1:449258/1‑120 (MQ=255)
ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt > 3:75882/1‑120 (MQ=255)
ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt < 4:75882/120‑1 (MQ=255)
ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt < 2:449258/120‑1 (MQ=255)
ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt < 1:1353833/118‑1 (MQ=255)
ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt > 2:1353833/1‑118 (MQ=255)
ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCAAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTc < 1:751018/116‑1 (MQ=255)
gCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat > 2:455993/1‑114 (MQ=255)
gCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat < 1:455993/114‑1 (MQ=255)
aaccATCCAAATCTGGATGGCTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt > 2:596421/3‑93 (MQ=38)
aaccATCCAAATCTGGATGGCTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt < 1:596421/91‑1 (MQ=38)
aaccATCCAAATCTGGATGG‑CTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTa < 2:1253695/130‑1 (MQ=255)
aaccATCCAAATCTGGATGG‑CTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGACGCTa > 1:1253695/3‑132 (MQ=255)
aTCCAGGTTTGGATGG‑TTTTTTGTTGTCTATATCTGGTGAGCTAATTACGAATCGTAAATATTTATTTTATTAATATTTTAACTTAATCTATATGGCATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTa > 2:423437/1‑149 (MQ=255)
|
CGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTA > NC_000913/4409893‑4410177
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
