Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F70 I0 R1 565 64.8 2371218 98.3% 2330907 140.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,395,455 G→A 12.2% A464T (GCA→ACA)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,395,4550GA12.2% 137.5 / 9.9 49A464T (GCA→ACA) nnrbifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand):  ref base G (19/24);  new base A (3/3);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTC  >  NC_000913/4395307‑4395603
                                                                                                                                                    |                                                                                                                                                    
cGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtgtg                                                                                                                                                      >  3:3863/1‑149 (MQ=255)
  gAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCa                                                                                                                                                    >  2:105100/1‑149 (MQ=255)
           tCGCCGTCCATCCTGCCGCTTTAGGCATTATTGTTGCCGGAAATGTAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTc                                                                                                                                           <  1:461934/149‑1 (MQ=255)
             gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGTGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgc                                                                                                                                         <  3:257727/149‑1 (MQ=255)
             gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGCACAGGCtgcatcat                                                                                                                                         <  3:152268/149‑7 (MQ=255)
                  ccATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACg                                                                                                                                    >  2:272155/1‑149 (MQ=255)
                    aTCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGt                                                                                                                                  <  2:344388/149‑1 (MQ=255)
                      ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGAAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGc                                                                                                                                <  3:150698/149‑1 (MQ=255)
                         gACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGc                                                                                                                             >  4:21658/1‑149 (MQ=255)
                                  ggCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGt                                                                                                                    >  2:389688/1‑149 (MQ=255)
                                   gCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTa                                                                                                                   <  2:393128/149‑1 (MQ=255)
                                    cATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGa                                                                                                                       >  3:327775/1‑144 (MQ=255)
                                    cATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGa                                                                                                                       <  4:327775/144‑1 (MQ=255)
                                                     aTGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGa                                                                                                 <  2:352821/149‑1 (MQ=255)
                                                      tGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGaa                                                                                                <  1:317292/149‑1 (MQ=255)
                                                                 cGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATg                                                                                     <  2:73851/149‑1 (MQ=255)
                                                                         ggcATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAAc                                                                             <  1:531040/149‑1 (MQ=255)
                                                                           cATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCg                                                                           >  2:541864/1‑149 (MQ=255)
                                                                               ggCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGAtct                                                                       <  4:455688/149‑1 (MQ=255)
                                                                                     gtgCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTc                                                                 <  3:563003/149‑1 (MQ=255)
                                                                                        ctctctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCAc                                                              >  4:480558/1‑149 (MQ=255)
                                                                                         tctctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACg                                                             >  2:462714/1‑149 (MQ=255)
                                                                                           tctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCt                                                           >  3:220524/1‑149 (MQ=255)
                                                                                              ggTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACa                                                        <  2:368554/149‑1 (MQ=255)
                                                                                                  ttattGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGt                                                    >  2:24775/1‑149 (MQ=255)
                                                                                                     ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGAtctc                                                                      <  2:427407/128‑1 (MQ=255)
                                                                                                     ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGAtctc                                                                      >  1:427407/1‑128 (MQ=255)
                                                                                                     ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTa                                                   >  4:27658/1‑147 (MQ=255)
                                                                                                     ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTa                                                   <  3:27658/147‑1 (MQ=255)
                                                                                                       ggCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGt                                               <  1:25426/149‑1 (MQ=255)
                                                                                                       ggCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGt                                               <  1:462714/149‑1 (MQ=255)
                                                                                                        gcgcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGtt                                              >  4:562420/1‑149 (MQ=255)
                                                                                                                 ttGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGTTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGaa                                     <  1:105100/149‑1 (MQ=255)
                                                                                                                 ttGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGaa                                     <  3:480558/149‑1 (MQ=255)
                                                                                                                 ttGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGaa                                     <  3:420933/149‑1 (MQ=255)
                                                                                                                     gCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGa                                 >  1:274665/1‑149 (MQ=255)
                                                                                                                      cAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGAc                                <  2:233854/149‑1 (MQ=255)
                                                                                                                      cAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGAc                                <  1:299093/149‑1 (MQ=255)
                                                                                                                       aaaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTa                                                          <  2:585671/122‑1 (MQ=255)
                                                                                                                       aaaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTa                                                          >  1:585671/1‑122 (MQ=255)
                                                                                                                                  ccGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCa                    >  3:63285/1‑149 (MQ=255)
                                                                                                                                      atgatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatgat                <  4:377205/149‑1 (MQ=255)
                                                                                                                                        gatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCAtgatga              >  2:98149/1‑149 (MQ=255)
                                                                                                                                        gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTg                              >  3:497203/5‑133 (MQ=255)
                                                                                                                                        gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTg                              <  4:497203/129‑1 (MQ=255)
                                                                                                                                        gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCAtgatga              >  1:510473/5‑149 (MQ=255)
                                                                                                                                          tgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAAt            >  1:484500/1‑149 (MQ=255)
                                                                                                                                          cacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCat                   >  4:21339/3‑142 (MQ=255)
                                                                                                                                          cacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCat                   <  3:21339/140‑1 (MQ=255)
                                                                                                                                           gCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATc           >  1:431538/1‑149 (MQ=255)
                                                                                                                                                  gtgCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAAt    <  4:63285/149‑1 (MQ=255)
                                                                                                                                                   tgCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAAtt   <  1:315867/149‑1 (MQ=255)
                                                                                                                                                    gCAGGCTGTGTCGCGCACTGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTc  <  1:525571/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTC  >  NC_000913/4395307‑4395603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A