Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F216 I0 R1
|
247 |
44.8 |
1629152 |
98.0% |
1596568 |
139.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,608,704 |
A→G |
13.3% |
S107P (TCG→CCG) |
yneE ← |
bestrophin family putative inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,608,704 | 0 | A | G | 13.3%
| 73.8
/ 4.9
| 30 | S107P (TCG→CCG) | yneE | bestrophin family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (9/17); new base G (2/2); total (11/19) |
| Fisher's exact test for biased strand distribution p-value = 6.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 |
TCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGA > NC_000913/1608566‑1608844
|
tCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCaa < 3:128561/148‑1 (MQ=255)
gCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCaagtgta < 3:175585/148‑6 (MQ=255)
cGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTc < 1:294821/149‑1 (MQ=255)
tGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGa < 3:312514/148‑1 (MQ=255)
tGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGa < 4:120069/148‑1 (MQ=255)
tAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCa < 3:104323/109‑1 (MQ=255)
tAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGGATTCGGCa > 4:104323/1‑109 (MQ=255)
gAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAatcatc < 1:61544/149‑1 (MQ=255)
cAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACt < 2:264217/149‑1 (MQ=255)
cTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGccc > 3:165629/1‑148 (MQ=255)
ccTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAg > 4:43132/1‑148 (MQ=255)
tgGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGtttt < 3:391515/148‑1 (MQ=255)
tGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTcgcg < 3:184828/148‑1 (MQ=255)
gTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAg < 3:54115/105‑1 (MQ=255)
gTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAg > 4:54115/1‑105 (MQ=255)
cGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGt > 4:371176/1‑148 (MQ=255)
tAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTAc > 3:11743/1‑148 (MQ=255)
aGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCa < 1:76021/149‑1 (MQ=255)
gTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCa < 4:11743/148‑1 (MQ=255)
ggCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGa > 4:27248/1‑87 (MQ=255)
ggCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGATTTTACCTCGCGCAGTAACGACCGTGa < 3:27248/87‑1 (MQ=255)
aGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGaa < 1:268662/149‑1 (MQ=255)
cGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGaaaaa < 4:93250/148‑1 (MQ=255)
ggCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatg < 4:165629/148‑1 (MQ=255)
gCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTCTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAatgg < 4:253005/148‑1 (MQ=255)
ttACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGaca > 2:182691/1‑149 (MQ=255)
acattGCCGGATTCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACAcc > 4:9158/4‑148 (MQ=255)
cTTGCCGAATCCGGCAATGTCGTTTTTGCCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCga > 4:248262/1‑148 (MQ=255)
ttGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCatta > 1:29975/1‑110 (MQ=255)
ttGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCatta < 2:29975/110‑1 (MQ=255)
|
TCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGACACCGA > NC_000913/1608566‑1608844
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
