Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A25 F36 I0 R2	1302	104.9	4551290	96.3%	4382892	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,706,750:1	+T	5.3%	coding (403/579 nt)	rsxB →	SoxR iron‑sulfur cluster reduction factor component; putative iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,706,750	1	.	T	5.3%	336.3 / 13.1	75	coding (403/579 nt)	rsxB	SoxR iron‑sulfur cluster reduction factor component; putative iron‑sulfur protein
Reads supporting (aligned to +/- strand): ref base . (36/35); new base T (2/2); total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

TAATGTCGAGCCGCAGCCGCTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACAC  >  NC_000913/1706614‑1706886

tAATGTCGAGCCGCAGCCGCTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGcc                                                                                                                               >  4:806568/1‑148 (MQ=255)

               gccgcTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATg                                                                                                                <  3:806568/148‑1 (MQ=255)

                                   aaGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATa                                                                                                                        <  2:307241/120‑1 (MQ=255)

                                   aaGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATa                                                                                                                        >  1:307241/1‑120 (MQ=255)

                                    agagATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAga                                                                                                             <  1:781076/130‑2 (MQ=255)

                                    agagATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGt                                                                                                             >  2:781076/1‑130 (MQ=255)

                                         tAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAAttt                                                                                      >  2:210608/1‑148 (MQ=255)

                                         tAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAAttt                                                                                      >  3:226049/1‑148 (MQ=255)

                                           aCGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATg                                                                                                                >  1:8497/1‑120 (MQ=255)

                                           aCGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATg                                                                                                                <  2:8497/120‑1 (MQ=255)

                                            cGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTAtg                                                                                   <  4:226049/148‑1 (MQ=255)

                                                tGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAg                                                                                                              <  1:449358/117‑1 (MQ=255)

                                                tGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAg                                                                                                              >  2:449358/1‑117 (MQ=255)

                                                    cgGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCa                                                                                          <  3:274243/133‑1 (MQ=255)

                                                    cgGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCa                                                                                          >  4:274243/1‑133 (MQ=255)

                                                             gCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGa                                                                  <  1:210608/148‑1 (MQ=255)

                                                              cGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGAtctc                                                                                                       <  3:1131885/110‑1 (MQ=255)

                                                              cGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGAtctc                                                                                                       >  4:1131885/1‑110 (MQ=255)

                                                              cGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGc                                                                                              <  2:585512/119‑1 (MQ=255)

                                                              cGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGc                                                                                              >  1:585512/1‑119 (MQ=255)

                                                                        tgaAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTaa                                                                                                                  >  1:954881/1‑89 (MQ=255)

                                                                        tgaAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTaa                                                                                                                  <  2:954881/89‑1 (MQ=255)

                                                                        tgaAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGc                                                                                              <  4:1065318/109‑1 (MQ=255)

                                                                        tgaAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGc                                                                                              >  3:1065318/1‑109 (MQ=255)

                                                                          aaaaTAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATg                                                                                                                            <  2:559304/77‑1 (MQ=255)

                                                                          aaaaTAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATg                                                                                                                            >  1:559304/1‑77 (MQ=255)

                                                                                       tGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCa                                               <  3:600704/141‑1 (MQ=255)

                                                                                       tGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCa                                               >  4:600704/1‑141 (MQ=255)

                                                                                          cTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGc                                                                                                                           <  1:833329/62‑1 (MQ=255)

                                                                                          cTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGc                                                                                                                           >  2:833329/1‑62 (MQ=255)

                                                                                          cTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACggg                                                                                               >  2:247089/1‑90 (MQ=255)

                                                                                          cTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACggg                                                                                               <  1:247089/90‑1 (MQ=255)

                                                                                            gCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGa                                   >  2:742325/1‑148 (MQ=255)

                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATg                                                                                                                <  1:199881/67‑1 (MQ=255)

                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATg                                                                                                                >  2:199881/1‑67 (MQ=255)

                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAt                                                        <  4:680909/123‑1 (MQ=255)

                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAt                                                        >  3:680909/1‑123 (MQ=255)

                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               <  4:755101/148‑1 (MQ=255)

                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               >  3:755101/1‑148 (MQ=255)

                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               <  1:742325/148‑1 (MQ=255)

                                                                                                 aaaTGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTAtg                                                                                   >  1:36790/1‑95 (MQ=255)

                                                                                                 aaaTGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTAtg                                                                                   <  2:36790/95‑1 (MQ=255)

                                                                                                   aTGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAtct                                                      <  2:708193/122‑1 (MQ=255)

                                                                                                   aTGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAtct                                                      >  1:708193/1‑122 (MQ=255)

                                                                                                    tGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATg                                                                                                                <  1:747198/63‑1 (MQ=255)

                                                                                                    tGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATg                                                                                                                >  2:747198/1‑63 (MQ=255)

                                                                                                      tATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACg                                                                <  3:1076783/109‑1 (MQ=255)

                                                                                                      tATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACg                                                                >  4:1076783/1‑109 (MQ=255)

                                                                                                          cAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCt                     >  1:737005/1‑148 (MQ=255)

                                                                                                            ggCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               >  1:352314/1‑136 (MQ=255)

                                                                                                            ggCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               <  2:352314/136‑1 (MQ=255)

                                                                                                              cGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 >  1:789413/1‑148 (MQ=255)

                                                                                                                    cGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGa           <  2:737005/148‑1 (MQ=255)

                                                                                                                      gTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAtct                                                      >  3:25165/1‑103 (MQ=255)

                                                                                                                      gTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAtct                                                      <  4:25165/103‑1 (MQ=255)

                                                                                                                       tAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGAt          <  3:264745/146‑1 (MQ=255)

                                                                                                                       tAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGAt          >  4:264745/1‑146 (MQ=255)

                                                                                                                        tagcGCCAACGATGGCGTCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 <  1:150671/136‑1 (MQ=255)

                                                                                                                        tagcGCCAACGATGGCGTCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 >  2:150671/4‑139 (MQ=255)

                                                                                                                        aGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTg       >  3:875868/1‑148 (MQ=255)

                                                                                                                           cGCCAACGATGGCGTCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGa           >  4:270824/1‑142 (MQ=255)

                                                                                                                           cGCCAACGATGGCGTCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGa           <  3:270824/142‑1 (MQ=255)

                                                                                                                             ccATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAacac  <  2:789413/148‑1 (MQ=255)

                                                                                                                              cATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAAt                                                                                        <  3:734003/61‑1 (MQ=255)

                                                                                                                              cATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAAt                                                                                        >  4:734003/1‑61 (MQ=255)

                                                                                                                               aTCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 >  1:420870/1‑131 (MQ=255)

                                                                                                                               aTCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 <  2:420870/131‑1 (MQ=255)

                                                                                                                               aTCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATg              <  2:266905/134‑1 (MQ=255)

                                                                                                                               aTCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATg              >  1:266905/1‑134 (MQ=255)

                                                                                                                                   ttGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATggg            >  4:159402/1‑132 (MQ=255)

                                                                                                                                   ttGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATggg            <  3:159402/132‑1 (MQ=255)

                                                                                                                                    tGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGt                                                   >  1:442006/1‑92 (MQ=255)

                                                                                                                                    tGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGt                                                   <  2:442006/92‑1 (MQ=255)

                                                                                                                                    tGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATg              <  3:853410/129‑1 (MQ=255)

                                                                                                                                    tGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATg              >  4:853410/1‑129 (MQ=255)

TAATGTCGAGCCGCAGCCGCTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCG‑CTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACAC  >  NC_000913/1706614‑1706886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A