Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F44 I0 R1	387	90.4	3244086	98.5%	3195424	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,703,252	A→T	9.5%	intergenic (+18/+16)	add → / ← ydgJ	adenosine deaminase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,703,252	0	A	T	9.5%	138.0 / 6.2	42	intergenic (+18/+16)	add/ydgJ	adenosine deaminase/putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (18/20); new base T (2/2); total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

CGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCA  >  NC_000913/1703108‑1703400

cGTTGCAGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCa                                                                                                                                                  >  2:578173/1‑149 (MQ=255)

     ccGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCAccatc                                                                                                                                             <  4:330292/149‑1 (MQ=255)

     ccGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCAccatc                                                                                                                                             <  2:318079/149‑1 (MQ=255)

     ccGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCAcca                                                                                                                                               >  2:50590/1‑147 (MQ=255)

     ccGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCAcca                                                                                                                                               <  3:664704/147‑1 (MQ=255)

     ccGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCAcca                                                                                                                                               >  4:664704/1‑147 (MQ=255)

     ccGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCAcca                                                                                                                                               <  1:50590/147‑1 (MQ=255)

                  tgGGTTATACCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCa                                                                                                                                >  2:310295/1‑149 (MQ=255)

                   gggTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCaa                                                                                                                               >  3:539710/1‑149 (MQ=255)

                    ggTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAg                                                                                                                              >  2:198768/1‑149 (MQ=255)

                      ttATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccatc                                                                                                                                             <  3:548191/132‑1 (MQ=255)

                      ttATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccatc                                                                                                                                             >  4:548191/1‑132 (MQ=255)

                       tATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGca                                                                                                                           <  1:578173/149‑1 (MQ=255)

                        aTCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGcac                                                                                                                          >  3:681980/1‑149 (MQ=255)

                           ccGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACaaa                                                                                                                       >  1:75769/1‑149 (MQ=255)

                           ccGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACaaa                                                                                                                       <  3:682668/149‑1 (MQ=255)

                                  cAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTcgcgc                                                                                                                <  1:689115/149‑1 (MQ=255)

                                       ccGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccatc                                                                                                                                             <  4:475215/115‑1 (MQ=255)

                                       ccGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAccatc                                                                                                                                             >  3:475215/1‑115 (MQ=255)

                                         gCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGttt                                                                                                         >  4:193650/1‑149 (MQ=255)

                                           cAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTgg                                                                                                       <  4:539710/149‑1 (MQ=255)

                                                    aTTAATGGTCTGGCAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGa                                                                                              <  4:681980/149‑1 (MQ=255)

                                                        aTGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGc                                                                                                      >  3:328135/1‑137 (MQ=255)

                                                        aTGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCACCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGc                                                                                                      <  4:328135/137‑1 (MQ=255)

                                                              tGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtc                                                                                    >  4:715075/1‑149 (MQ=255)

                                                                 aaaTGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtcaat                                                                                 >  4:249525/1‑149 (MQ=255)

                                                                  aaTGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtcaatc                                                                                <  3:193650/149‑1 (MQ=255)

                                                                     ggCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAAc                                                                             <  1:310295/149‑1 (MQ=255)

                                                                       cTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTc                                                                           <  3:273399/149‑1 (MQ=255)

                                                                        tttCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTcc                                                                          >  4:337736/1‑149 (MQ=255)

                                                                            cTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTa                                                                      >  3:218564/1‑149 (MQ=255)

                                                                                       ggAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGcc                                                           <  1:198768/149‑1 (MQ=255)

                                                                                       ggAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGcc                                                           <  2:75769/149‑1 (MQ=255)

                                                                                       ggAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGcc                                                           <  4:218564/149‑1 (MQ=255)

                                                                                                        gagaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCgg                                          >  4:302533/1‑149 (MQ=255)

                                                                                                        gagaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCgg                                          >  2:536185/1‑149 (MQ=255)

                                                                                                         agaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGa                                         <  1:334340/149‑1 (MQ=255)

                                                                                                          gaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGAt                                        >  3:739530/1‑149 (MQ=255)

                                                                                                          gaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGAt                                        <  1:536185/149‑1 (MQ=255)

                                                                                                          gaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGAt                                        >  3:605285/1‑149 (MQ=255)

                                                                                                             aaaGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGAtttt                                     <  1:480530/149‑1 (MQ=255)

                                                                                                              aaGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTc                                    <  3:715075/149‑1 (MQ=255)

                                                                                                                                                aTGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCa  >  2:788171/1‑149 (MQ=255)

CGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGCCGCA  >  NC_000913/1703108‑1703400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A