Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F68 I0 R1 683 78.3 2772122 98.0% 2716679 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,334,703 A→G 12.3% intergenic (+22/‑206) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,334,7030AG12.3% 197.0 / 18.4 74intergenic (+22/‑206)ispB/sfsBoctaprenyl diphosphate synthase/malPQ operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (28/36);  new base G (5/4);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-02

ACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGA  >  NC_000913/3334559‑3334843
                                                                                                                                                |                                                                                                                                             
aCGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgc‑gc                                                                                                                                          >  4:164919/1‑149 (MQ=255)
  gcgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgc‑gcgc                                                                                                                                        >  4:615175/1‑149 (MQ=255)
    gTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCgg                                                                                                                                      <  2:155906/149‑1 (MQ=255)
    gTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCgg                                                                                                                                      <  1:645290/149‑1 (MQ=255)
     tCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCggg                                                                                                                                     >  3:195346/1‑149 (MQ=255)
      cAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGa                                                                                                                                    <  1:577499/149‑1 (MQ=255)
           tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGtt                                                                                                                               >  3:281261/1‑149 (MQ=255)
              cGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCa                                                                                                                            <  3:3586/149‑1 (MQ=255)
                  gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTaa                                                                                                                        >  3:463089/1‑149 (MQ=255)
                     gaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGa                                                                                                                                    >  2:692069/1‑134 (MQ=255)
                     gaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGa                                                                                                                                    <  1:692069/134‑1 (MQ=255)
                        gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCa                                                                                                                  <  4:195346/149‑1 (MQ=255)
                        gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCa                                                                                                                  >  1:557194/1‑149 (MQ=255)
                                aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATGGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAaca                                                                                                          <  1:119040/149‑1 (MQ=255)
                                aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCAGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAaca                                                                                                          <  1:208975/149‑1 (MQ=255)
                                  ccATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACt                                                                                                        >  2:198142/1‑149 (MQ=255)
                                    aTCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTa                                                                                                      >  2:117817/1‑149 (MQ=255)
                                     tCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGAt                                                                                                                                   >  4:470254/1‑119 (MQ=255)
                                     tCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCa                                                                                                                            <  1:332541/126‑1 (MQ=255)
                                     tCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCa                                                                                                                            >  2:332541/1‑126 (MQ=255)
                                     tCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAt                                                                                                     <  4:506473/149‑1 (MQ=255)
                                     tCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAt                                                                                                     >  3:384951/1‑149 (MQ=255)
                                     tCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAACCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGAt                                                                                                                                   <  3:470254/119‑1 (MQ=255)
                                      cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAtt                                                                                                    <  3:615175/149‑1 (MQ=255)
                                      cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAtt                                                                                                    <  1:198142/149‑1 (MQ=255)
                                          gCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGc                                                                                                >  4:443013/1‑149 (MQ=255)
                                            gTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGctc                                                                                              >  2:292228/1‑149 (MQ=255)
                                              tACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCta                                                                                            <  4:384951/149‑1 (MQ=255)
                                               aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCtat                                                                                           <  3:164919/149‑1 (MQ=255)
                                                    tGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCT‑GCGCGGGATgag                                                                                                                                >  1:488321/1‑105 (MQ=255)
                                                    tGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCT‑GCGCGGGATgag                                                                                                                                <  2:488321/107‑3 (MQ=255)
                                                       tCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa                                                                                                            <  3:389504/124‑1 (MQ=255)
                                                       tCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa                                                                                                            >  4:389504/1‑124 (MQ=255)
                                                       tCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTg                                                                                   >  2:71070/1‑149 (MQ=255)
                                                           ggACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaaaa                                                                               >  3:242/1‑149 (MQ=255)
                                                            gACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaaaaa                                                                              <  1:117817/149‑1 (MQ=255)
                                                              cacCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                            <  2:661894/149‑1 (MQ=255)
                                                              cacCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                            <  1:71070/149‑1 (MQ=255)
                                                                ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgg                                                                          <  1:413396/149‑1 (MQ=255)
                                                                 cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                            <  1:20209/146‑1 (MQ=255)
                                                                 cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                            >  2:20209/1‑146 (MQ=255)
                                                                 cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                         >  1:213890/1‑149 (MQ=255)
                                                                  ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa                                                                        >  4:133508/1‑149 (MQ=255)
                                                                  ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa                                                                        <  3:443013/149‑1 (MQ=255)
                                                                   cTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATATCACCTGAAAAATAGGTAt                                                                       <  4:463089/149‑1 (MQ=255)
                                                                     tGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAAt                                                                             >  1:147120/1‑141 (MQ=255)
                                                                     tGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAAt                                                                             <  2:147120/141‑1 (MQ=255)
                                                                         gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCT‑GCGCGGGATga                                                                                                                                 <  2:626774/85‑2 (MQ=38)
                                                                         gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCT‑GCGCGGGATga                                                                                                                                 >  1:626774/1‑84 (MQ=38)
                                                                              gCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta                                                            >  1:410681/1‑149 (MQ=255)
                                                                                    aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt                                                      <  3:133508/149‑1 (MQ=255)
                                                                                     tCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTc                                                     <  2:213890/149‑1 (MQ=255)
                                                                                       ggCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTg                                                   <  2:456855/149‑1 (MQ=255)
                                                                                            cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAatat                                              <  2:645885/149‑1 (MQ=255)
                                                                                              cgcACATCGCTGTTCAACGCGATCGTTAATCCCATCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCAGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTc                                            <  4:644338/149‑1 (MQ=255)
                                                                                                          ttCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACa                                <  4:329794/149‑1 (MQ=255)
                                                                                                             aaCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGa                             >  4:487088/1‑149 (MQ=255)
                                                                                                                 cgATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtt                         <  2:410681/149‑1 (MQ=255)
                                                                                                                  gATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttt                        <  1:292228/149‑1 (MQ=255)
                                                                                                                   aTCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt                       >  4:667355/1‑149 (MQ=255)
                                                                                                                    tCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttttt                      >  1:168776/1‑149 (MQ=255)
                                                                                                                           tcccctccccTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCaa               <  4:281261/149‑1 (MQ=255)
                                                                                                                             ccctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAac                                                                                        >  1:611098/1‑74 (MQ=255)
                                                                                                                             ccctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAac                                                                                        <  2:611098/74‑1 (MQ=255)
                                                                                                                               ctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAgg           >  3:546793/1‑149 (MQ=255)
                                                                                                                                tcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGc          <  4:242/149‑1 (MQ=255)
                                                                                                                                  ccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCa        >  4:193491/1‑149 (MQ=255)
                                                                                                                                    ctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATc      <  3:667355/149‑1 (MQ=255)
                                                                                                                                      cATCCCGCGC‑GCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAg    >  3:242344/1‑149 (MQ=255)
                                                                                                                                        tCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa  <  1:412740/149‑1 (MQ=255)
                                                                                                                                        tCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa  >  1:661238/1‑149 (MQ=255)
                                                                                                                                        tCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa  >  2:412740/1‑149 (MQ=255)
                                                                                                                                        tCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa  <  4:320109/149‑1 (MQ=255)
                                                                                                                                        tCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa  >  3:320109/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                             
ACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGA  >  NC_000913/3334559‑3334843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A