Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F195 I0 R2	483	52.1	1908604	98.1%	1872340	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,763,451	C→T	10.2%	V187M (GTG→ATG)	sufB ←	component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,763,451	0	C	T	10.2%	113.4 / 3.7	39	V187M (GTG→ATG)	sufB	component of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand): ref base C (19/16); new base T (2/2); total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCAC  >  NC_000913/1763308‑1763592

cTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGc                                                                                                                                          <  2:208199/149‑1 (MQ=255)

cTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGc                                                                                                                                          <  3:431834/149‑1 (MQ=255)

   cccGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCga                                                                                                                                       <  2:37270/149‑1 (MQ=255)

     cGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCga                                                                                                                                       >  1:140535/1‑147 (MQ=255)

     cGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCga                                                                                                                                       <  2:140535/147‑1 (MQ=255)

                   ttAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCGTGGTGCCggg                                                                                                                                      >  1:426338/1‑132 (MQ=255)

                   ttAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCGTGGTGCCggg                                                                                                                                      <  2:426338/134‑3 (MQ=255)

                      aTGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTc                                                                                                                    <  2:277669/149‑1 (MQ=255)

                           aaaaTAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGt                                                                                                               >  2:76624/1‑149 (MQ=255)

                                    ggAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGa                                                                                                      >  1:338992/1‑149 (MQ=255)

                                         gTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGcc                                                                                                 >  3:193708/1‑149 (MQ=255)

                                              aTCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCAcc                                                                                            >  3:143673/1‑149 (MQ=255)

                                                   gCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACTGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAgg                                                                                       >  4:234898/1‑149 (MQ=255)

                                                     aGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCATGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGaa                                                                                     >  1:5919/1‑149 (MQ=255)

                                                     aGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGaa                                                                                     <  4:143673/149‑1 (MQ=255)

                                                          aCGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGaa                                                                                >  1:298441/1‑149 (MQ=255)

                                                          aCGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGaa                                                                                >  1:288928/1‑149 (MQ=255)

                                                               tttAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataa                                                                           <  3:458914/149‑1 (MQ=255)

                                                                           aataaaCGTACCATCAGAAGCTACCGCCGCATTAAGCGCGGAAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTACGGGTGATCGTGGATCGCCTCACCAAAGGACCAGAAAATAATTCCCTGCTCCg                                                               >  2:316780/1‑149 (MQ=255)

                                                                                   tACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCATGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGtttt                                                       <  2:5919/149‑1 (MQ=255)

                                                                                       aTCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTcc                                                                       >  1:167589/1‑129 (MQ=255)

                                                                                       aTCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTcc                                                                       <  2:167589/129‑1 (MQ=255)

                                                                                       aTCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCATGCTCCGCCAGTTTTTcgc                                                   >  4:324339/1‑149 (MQ=255)

                                                                                                tACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagtag                                          <  3:324339/149‑1 (MQ=255)

                                                                                                  ccgccgCATTAAGCGCGGCAAAGAAGTTGTCATACCCCGGCACCACGGTGCCGAGATAGTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTg                                        >  4:455978/1‑149 (MQ=255)

                                                                                                     ccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCa                                     >  1:322984/1‑149 (MQ=255)

                                                                                                      cgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCaa                                    <  1:76624/149‑1 (MQ=255)

                                                                                                        cATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAAcc                                  >  4:353957/1‑149 (MQ=255)

                                                                                                             aGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAAc                             >  2:399544/1‑149 (MQ=255)

                                                                                                                gcgGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGTACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGa                          <  2:288928/149‑1 (MQ=255)

                                                                                                                     aaaGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagt                                            >  3:414158/1‑126 (MQ=255)

                                                                                                                     aaaGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagt                                            <  4:414158/126‑1 (MQ=255)

                                                                                                                     aaaGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGa                     >  2:56866/1‑149 (MQ=255)

                                                                                                                        gaagTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGaaaa                  <  2:298441/149‑1 (MQ=255)

                                                                                                                                     cccGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAAt                 >  1:384637/1‑137 (MQ=255)

                                                                                                                                     cccGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAAt                 <  2:384637/137‑1 (MQ=255)

                                                                                                                                      ccGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGcc    <  4:193708/149‑1 (MQ=255)

                                                                                                                                      ccGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGGTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGcc    >  2:190152/1‑149 (MQ=255)

                                                                                                                                        ggCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCAc  <  1:56866/149‑1 (MQ=255)

CTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCAC  >  NC_000913/1763308‑1763592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A