Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F169 I1 R1	62	44.4	970160	98.1%	951726	147.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,018,612	G→A	R103* (CGA→TGA)	mgt ←	Monofunctional glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,018,612	0	G	A	100.0%	113.1 / NA	34	R103* (CGA→TGA)	mgt	Monofunctional glycosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/17); total (17/17)

GAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAAT  >  USA300TCH1516_ALE/2018473‑2018754

gAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCtt                                                                                                                                        >  3:70963/1‑148 (MQ=255)

       cATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCtt                                                                                                                                        >  1:6770/1‑141 (MQ=255)

       cATTATCATAAAAATAATTTTAGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAGATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCtt                                                                                                                                        >  1:35490/1‑141 (MQ=255)

          tATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTcaa                                                                                                                                     >  1:31784/1‑139 (MQ=255)

             cATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaaa                                                                                                                           <  4:165618/148‑1 (MQ=255)

                       atTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGCTTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTa                                                                                                                 >  4:387675/1‑148 (MQ=255)

                       atTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTa                                                                                                                 >  4:116463/1‑148 (MQ=255)

                             gACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATAc                                                                                                           <  3:249682/148‑1 (MQ=255)

                             gACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATAc                                                                                                           <  4:28842/148‑1 (MQ=255)

                               cAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATActc                                                                                                         >  4:229536/1‑148 (MQ=255)

                                  attgttgTGTAATGGTACTACCACCTTGCACATCTCCGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCCACTCCATGACGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACAt                                                                                                             <  1:14485/140‑1 (MQ=255)

                                         tgtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTa                                                                                               <  4:367589/148‑1 (MQ=255)

                                         tgtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTa                                                                                               <  4:298330/148‑1 (MQ=255)

                                                         ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAac                                                                               >  4:389325/1‑148 (MQ=255)

                                                         ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAac                                                                               >  4:188681/1‑148 (MQ=255)

                                                           ttGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGCACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAacta                                                                             >  3:300561/1‑148 (MQ=255)

                                                                caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACtttt                                                                        >  3:189712/1‑148 (MQ=255)

                                                                  tctcTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAacta                                                                             >  1:64718/1‑141 (MQ=255)

                                                                    tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                    <  3:11719/148‑1 (MQ=255)

                                                                    tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                    <  3:8328/148‑1 (MQ=255)

                                                                    tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                    <  3:266810/148‑1 (MQ=255)

                                                                                cGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCtt                                                        >  4:346156/1‑148 (MQ=255)

                                                                                cGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCtt                                                        <  4:9127/148‑1 (MQ=255)

                                                                                                  aGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCcac                                             <  2:37588/141‑1 (MQ=255)

                                                                                                  aGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                      <  3:229536/148‑1 (MQ=255)

                                                                                                  aGTTGTACCTTTCAAATCAAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                      <  3:316390/148‑1 (MQ=255)

                                                                                                    ttGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTTATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcgcg                                    <  3:189540/148‑1 (MQ=255)

                                                                                                                aaTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaaaaa                        <  3:235056/148‑1 (MQ=255)

                                                                                                                 aTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaaaaaa                       >  4:217701/1‑148 (MQ=255)

                                                                                                                          atgatgATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATa              <  3:81966/148‑1 (MQ=255)

                                                                                                                               gatTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAAt         >  3:262041/1‑148 (MQ=255)

                                                                                                                                atTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATa        <  3:372404/148‑1 (MQ=255)

                                                                                                                                 ttGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATaa       >  3:342315/1‑148 (MQ=255)

                                                                                                                                      gAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAAt  >  4:258506/1‑148 (MQ=255)

GAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAAT  >  USA300TCH1516_ALE/2018473‑2018754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A