Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F67 I1 R1 15 88.5 1847830 95.4% 1762829 135.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,722,719 C→T G13D (GGT→GAT)  yciC_3 ← Putative metal chaperone YciC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,722,7190CT100.0% 324.9 / NA 87G13D (GGT→GAT) yciC_3Putative metal chaperone YciC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/41);  total (46/41)

TCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTACCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGTT  >  USA300TCH1516_ALE/2722582‑2722845
                                                                                                                                         |                                                                                                                              
tCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGc                                                                                                                             <  1:107472/141‑1 (MQ=255)
  aGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCa                                                                                                                           <  1:42839/141‑1 (MQ=255)
  aGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATCATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGACCAAATGATTTAAGACAGTCGTTTTATCGcta                                                                                                                           >  4:138526/1‑139 (MQ=255)
   gTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCAc                                                                                                                          >  1:514424/1‑141 (MQ=255)
        cTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaa                                                                                                                     >  3:249420/1‑141 (MQ=255)
        cTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaa                                                                                                                     <  2:230359/141‑1 (MQ=255)
             aGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAAccc                                                                                                                >  2:282065/1‑141 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCATATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  2:199919/1‑141 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCt                                                                                                                        <  4:12027/128‑1 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCt                                                                                                                        >  3:11982/1‑128 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCt                                                                                                                        >  3:12027/1‑128 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCt                                                                                                                        <  4:11982/128‑1 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaaaa                                                                                                                   <  3:27736/133‑1 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaaaa                                                                                                                   >  4:27736/1‑133 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  1:587263/1‑141 (MQ=255)
                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  1:92160/1‑141 (MQ=255)
                    tCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCtata                                                                                                         <  3:183963/141‑1 (MQ=255)
                     cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:206221/141‑1 (MQ=255)
                     cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  4:100647/141‑1 (MQ=255)
                     cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:18356/141‑1 (MQ=255)
                     cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:21774/141‑1 (MQ=255)
                      ttCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataat                                                                                                       <  1:295991/141‑1 (MQ=255)
                         aGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCtata                                                                                                         >  4:165231/1‑136 (MQ=255)
                         aGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCtata                                                                                                         <  3:165231/136‑1 (MQ=255)
                         aGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAAc                                                                                                    >  4:119618/1‑141 (MQ=255)
                            tACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAt                                                                                                 <  1:191498/141‑1 (MQ=255)
                             aCTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtt                                                                                                >  3:7100/1‑141 (MQ=255)
                               tAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:95848/1‑141 (MQ=255)
                               tAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  4:56015/1‑141 (MQ=255)
                               tAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:600232/1‑141 (MQ=255)
                               tAAGGCACCATCAACACNCATTTTCCCANATTCNTTCNTGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCACCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:394922/1‑141 (MQ=255)
                                   gCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATc                                                                                          >  4:81388/1‑141 (MQ=255)
                                    cACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCt                                                                                         <  2:365809/141‑1 (MQ=255)
                                      ccATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGAAAAATAAAAACCCACCTATAATAACTATTTTCATCTGc                                                                                       <  2:519561/141‑1 (MQ=255)
                                         tCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGAc                                                                                    >  4:91339/1‑141 (MQ=255)
                                          cAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTAAGCGATTCCTTTAATGACTAAGAGAGCATATGACTTAAGACAGTCGTTATTTAGTAATTAATAAAAAGCCCTATAATAACTATTTTCATCTGCGAct                                                                                   >  1:94480/1‑141 (MQ=255)
                                           aaCACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGActc                                                                                  <  2:587263/141‑1 (MQ=255)
                                             cacTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACtctc                                                                                >  4:249076/1‑141 (MQ=255)
                                               cTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCCTCTGCGACTctcct                                                                              >  1:134744/1‑141 (MQ=255)
                                                   ttttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTta                                                                          <  1:281544/141‑1 (MQ=255)
                                                   ttttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTta                                                                          >  2:330489/1‑141 (MQ=255)
                                                   ttttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTta                                                                          <  3:56015/141‑1 (MQ=255)
                                                    tttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTtat                                                                         <  3:81388/141‑1 (MQ=255)
                                                       cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTctcc                                                                               <  2:579710/132‑1 (MQ=255)
                                                       cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTctcc                                                                               >  1:579710/1‑132 (MQ=255)
                                                       cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACt                                                                      >  1:200784/1‑141 (MQ=255)
                                                        ccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACtt                                                                     <  1:639664/141‑1 (MQ=255)
                                                         cAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTc                                                                    <  4:161493/141‑1 (MQ=255)
                                                                  tcatGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATa                                                           >  3:126505/1‑141 (MQ=255)
                                                                  tcatGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATa                                                           >  1:559908/1‑141 (MQ=255)
                                                                    atgatgACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGc                                                         <  3:119618/141‑1 (MQ=255)
                                                                        tgaCTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCatat                                                     <  2:407900/141‑1 (MQ=255)
                                                                          aCTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTc                                                   >  1:129569/1‑141 (MQ=255)
                                                                          aCTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTc                                                   <  4:186843/141‑1 (MQ=255)
                                                                           ctgctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTcc                                                  >  2:332595/1‑141 (MQ=255)
                                                                              ctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAag                                               <  3:91409/141‑1 (MQ=255)
                                                                              ctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAag                                               <  3:136773/141‑1 (MQ=255)
                                                                              ctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAag                                               <  3:179531/141‑1 (MQ=255)
                                                                                   ttCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCACCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagataga                                          <  2:394922/141‑1 (MQ=255)
                                                                                      aGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagata                                            <  1:3143/136‑1 (MQ=255)
                                                                                      aGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagata                                            >  2:3143/1‑136 (MQ=255)
                                                                                      aGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGAtct                                       <  3:105213/141‑1 (MQ=255)
                                                                                       gCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGAtctc                                      >  4:120754/1‑141 (MQ=255)
                                                                                         gATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCt                                    <  1:170422/141‑1 (MQ=255)
                                                                                           ttCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGcc                                                                                                                            >  3:109808/1‑51 (MQ=255)
                                                                                           ttCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGcc                                                                                                                            <  4:109808/51‑1 (MQ=255)
                                                                                             ccTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtg                                >  1:219011/1‑141 (MQ=255)
                                                                                              cTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtga                               <  2:129569/141‑1 (MQ=255)
                                                                                               tttAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtgac                              >  1:523109/1‑141 (MQ=255)
                                                                                                        tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90016/1‑141 (MQ=255)
                                                                                                        tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90076/1‑141 (MQ=255)
                                                                                                        tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:189611/1‑141 (MQ=255)
                                                                                                        tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90005/1‑141 (MQ=255)
                                                                                                           gCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGTCATGActct                  <  4:132852/141‑1 (MQ=255)
                                                                                                               gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATCATAACTATTTTCATCTGCGCCTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              <  1:569173/141‑1 (MQ=255)
                                                                                                               gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              <  2:200784/141‑1 (MQ=255)
                                                                                                               gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              >  2:625810/1‑141 (MQ=255)
                                                                                                                cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCta                 <  1:380379/137‑1 (MQ=255)
                                                                                                                cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCta                 >  2:380379/1‑137 (MQ=255)
                                                                                                                cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATAtt             <  2:594180/141‑1 (MQ=255)
                                                                                                                 aaaTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTc            >  1:507220/1‑141 (MQ=255)
                                                                                                                 aaaTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTc            >  3:204220/1‑141 (MQ=255)
                                                                                                                   aTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAg          <  1:330489/141‑1 (MQ=255)
                                                                                                                   aTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATACATCTCCTATTGACTGACATGACTCTATATTCAg          >  2:121869/1‑141 (MQ=255)
                                                                                                                          aaGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGt   >  2:137047/1‑141 (MQ=255)
                                                                                                                           aGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGtt  >  3:278024/1‑141 (MQ=255)
                                                                                                                           aGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGtt  >  1:624237/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                              
TCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTACCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGTT  >  USA300TCH1516_ALE/2722582‑2722845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A