Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I1 R1	31	90.0	1985866	95.2%	1890544	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,473	C→T	A67T (GCT→ACT)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,473	0	C	T	99.0%	309.8 / NA	99	A67T (GCT→ACT)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (45/53); minor base G (1/0); total (46/53)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCT  >  USA300TCH1516_ALE/1744335‑1744610

gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTa                                                                                                                                         >  2:335407/1‑141 (MQ=255)

gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTa                                                                                                                                         >  1:286930/1‑141 (MQ=255)

 ttGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTAc                                                                                                                                        <  2:93422/141‑1 (MQ=255)

 ttGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTAc                                                                                                                                        <  2:512222/141‑1 (MQ=255)

  tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATCCTCATAACGAATGAATTCACGAGGAAATTTCCCTGCTTTTCTAAAACATTCAAAGGCAATCCCCATTGAATAAGTTACa                                                                                                                                       >  2:336418/1‑141 (MQ=255)

            tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCTTATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACCGCCat                                                                                                                             <  2:554350/141‑2 (MQ=255)

            tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATAACAATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa                                                                                                                             <  1:468883/141‑1 (MQ=255)

               ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGa                                                                                                                          <  2:804569/141‑1 (MQ=255)

                  cATCTTTGTGCATTGTTAAAACATTTGTAACATATTCAAGGTCATAATCATAACGAATGACTTCACGAGGTAATTTCGATCCTATTCGAATAGCTGCAAATCCAATCCCCATGGAGTAAGTTACAGGACAGCCAATGATaa                                                                                                                       >  2:724042/1‑141 (MQ=255)

                      tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGcc                                                                                                                   <  1:831674/141‑1 (MQ=255)

                         gtgCATTGTTAAATCATTTTTACCATATTCTAGGTCATACTCATACCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAcg                                                                                                                <  2:426022/141‑1 (MQ=255)

                         gtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAcg                                                                                                                <  1:503133/141‑1 (MQ=255)

                         gtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAcg                                                                                                                >  1:893754/1‑141 (MQ=255)

                                    ataaaTTTGTAACATATTCTAAGTCATACTCATAACGAACTACTGCACGAGGTAATTTCCCCTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTc                                                                                                     <  2:434123/137‑1 (MQ=255)

                                    aaaCATTTGTACCATAGTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTCCAGGACAGCCAATGCTAAAGCCACGCGCTTCAGGTc                                                                                                     >  1:992424/1‑141 (MQ=255)

                                    aaaCATTTGTACCAAATTCAAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGGTACAGGCCAGCCAATGATACAGCCACGCGCTTCAGGTc                                                                                                     >  1:643948/1‑141 (MQ=255)

                                    aaaCATTTGGACCATCTTCTAGGTCATACTCATAACGAAGGACGTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTc                                                                                                     <  2:44486/141‑1 (MQ=255)

                                     aaCATTTGTACCTTATTCTAGGTCATACTCATAACGAATGACTTCATGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                                                                    <  1:298478/141‑1 (MQ=255)

                                     aaCATTTGTACCATATTCTAGGCCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                                                                    <  2:867497/141‑1 (MQ=255)

                                      aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                                                                   >  1:914716/1‑141 (MQ=255)

                                      aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                                                                   >  1:550053/1‑141 (MQ=255)

                                      aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                                                                   >  1:774688/1‑141 (MQ=255)

                                      aCATTTGTACCATATTCTAGGTCATACTCATAACGAACGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGACAATGATAAAGCCACGCGCTTCAGGTCCt                                                                                                   <  1:26162/141‑1 (MQ=255)

                                       cATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGTTAAAGCCACGCGCTTCAGGTCCTa                                                                                                  >  1:483648/1‑141 (MQ=255)

                                        aTTTGTACCATATTCTCGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACGGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTAc                                                                                                 <  2:935131/141‑1 (MQ=255)

                                         tttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTCCAGGACGGCCAATGATAAAGCCACGCGCTTCAGGTCCTACa                                                                                                >  2:166206/1‑141 (MQ=255)

                                              aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGat                                                                                           >  2:509566/1‑141 (MQ=255)

                                               ccATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATGGCATAAGTTACAGGACAGCCAATGATACAGCCACGCGCTTCAGGTCCTACAACGaga                                                                                          >  1:39485/1‑139 (MQ=255)

                                                    ttCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAc                                                                                     <  2:286930/141‑1 (MQ=255)

                                                       tAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtc                                                                                  >  1:657012/1‑141 (MQ=255)

                                                        aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                                                 <  1:307201/141‑1 (MQ=255)

                                                           tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGGTTTCAACATCTCTg                                                                              >  1:291531/1‑141 (MQ=255)

                                                           tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                              <  1:331573/141‑1 (MQ=255)

                                                           tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGAGAAAGCCACCCGCGTCAGGTCCTACAACGATATCAACATCTCTg                                                                              >  1:923219/1‑141 (MQ=255)

                                                           tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAAGGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                              >  1:29179/1‑141 (MQ=255)

                                                           ccATTCTCATGACTAGTGACTCTACGCGGTAATTTCCCTTCCTTTCCAACGTTTGCACATCCTATCCCCATTGATTAAGTAACAGGGCAGCCAATGACAAAGCCACGCTCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                              <  2:161902/140‑1 (MQ=255)

                                                            cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                             >  1:178963/1‑141 (MQ=255)

                                                                 tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtttt                                                                        <  1:84033/141‑1 (MQ=255)

                                                                 tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtttt                                                                        <  2:978010/141‑1 (MQ=255)

                                                                  cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                                       >  1:271218/1‑141 (MQ=255)

                                                                    tAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCGAAGCCCCATGGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGGCTCAGGTCCTACAACGATATCCACATCTCTGTCTTTTGCg                                                                     >  2:55148/1‑141 (MQ=255)

                                                                    tAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTGTCTTTTGCg                                                                     >  2:283863/1‑141 (MQ=255)

                                                                     aaCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAACTCTCTGTCGTTTGCGt                                                                    >  2:58777/1‑141 (MQ=255)

                                                                      aCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCACAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGGTATCAACATCTCTGTCTTTTCCGTa                                                                   >  1:169706/1‑141 (MQ=255)

                                                                      aCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGtt                                                                   >  1:833230/1‑140 (MQ=255)

                                                                      aCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTa                                                                   >  1:348514/1‑141 (MQ=255)

                                                                       cGAATGACTTCATGAAGTACTTTCCCTTCTTTTCTAACATGTGCAAAGCCAATCCCCAGTGAATAGGTTACAGGACAGCCAATGATAAAGCCACGCGCTGCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt                                                                  <  2:522469/141‑1 (MQ=255)

                                                                         aaTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTc                                                                >  1:657153/1‑141 (MQ=255)

                                                                            gACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTAc                                                             >  2:909585/1‑141 (MQ=255)

                                                                              cTTCGCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCCACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACaa                                                           >  1:699148/1‑141 (MQ=255)

                                                                              cTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGAGCTGCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACaa                                                           >  2:595236/1‑141 (MQ=255)

                                                                              cTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAACCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCGTCAGGTCCTACAACGCTATCAACATCTCTGTCTTTTGCCTATTCTACaa                                                           >  2:223665/1‑141 (MQ=255)

                                                                                 cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                                                        >  1:784805/1‑141 (MQ=255)

                                                                                    gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTAt                                                     <  1:663714/141‑1 (MQ=255)

                                                                                    gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACACCCCCCAGGTCCTGTAACGATATTAACATCTCTGTCTTTTGCGTCTTCTACAATTTTac                                                     >  2:389647/1‑140 (MQ=255)

                                                                                      ggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCt                                                   >  2:932018/1‑141 (MQ=255)

                                                                                         aaTTTCCCTTCGTTTCGACCCGGTGCAACGCCAATCCCCATTGAATAAGTTACAGGACCGCCAATGATACAGCCATGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt                                                <  2:915236/141‑1 (MQ=255)

                                                                                         aaTTTCCCTCCTTTTCTACCAGGTGCAAAGCCAACCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt                                                <  1:133304/141‑1 (MQ=255)

                                                                                           tttCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCATCAAATTTGTCTTTTga                                              >  1:540348/1‑140 (MQ=255)

                                                                                              cccTTCTTTTCTAACAAGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTATACAATTTTATCTGTTGCATa                                           >  1:494232/1‑141 (MQ=255)

                                                                                                cttcttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGc                                         <  1:399291/141‑1 (MQ=255)

                                                                                                 ttcttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATatgg                                        <  1:596483/141‑4 (MQ=255)

                                                                                                    ttttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata                                     <  1:509566/141‑1 (MQ=255)

                                                                                                       tCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAATCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGc                                  <  1:132069/141‑1 (MQ=255)

                                                                                                       tCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGc                                  <  2:863556/141‑1 (MQ=255)

                                                                                                       tCTAACAGGTGCAAAGCCAACCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGc                                  <  2:362691/141‑1 (MQ=255)

                                                                                                        cTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCt                                 <  2:151976/141‑1 (MQ=255)

                                                                                                         tAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCtt                                <  1:48888/141‑1 (MQ=255)

                                                                                                          aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                               <  1:458158/141‑1 (MQ=255)

                                                                                                          aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                               <  2:550053/141‑1 (MQ=255)

                                                                                                          aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                               <  2:774688/141‑1 (MQ=255)

                                                                                                          aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                               <  1:335407/141‑1 (MQ=255)

                                                                                                          aaCAGGTGCAAAGCCAATCCCCATCGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTCCATAGCCATATGCTTc                               <  2:115458/141‑1 (MQ=255)

                                                                                                           ccAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa                              <  2:712658/140‑1 (MQ=255)

                                                                                                           aCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCACTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCCTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa                              <  2:122315/141‑1 (MQ=255)

                                                                                                            cAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc                             >  2:83118/1‑141 (MQ=255)

                                                                                                            cAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc                             >  1:657205/1‑141 (MQ=255)

                                                                                                            cAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATATTATCTGTTGCATAGCCATATGCTTCAc                             >  1:215531/1‑141 (MQ=255)

                                                                                                              ggTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCa                           >  1:611840/1‑141 (MQ=255)

                                                                                                               gTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGAATATTTATTTTTTATCTTTTGCATAGCCATAGACTTCACCAt                          <  1:724042/141‑1 (MQ=255)

                                                                                                                 gCAAAGCCAATCCCCATTGAATAAGTTCCAGGACAGCCAATGATAAAGCCATGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTTTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTa                        <  2:643948/141‑1 (MQ=255)

                                                                                                                 gCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCGTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTa                        <  1:866129/141‑1 (MQ=255)

                                                                                                                  cAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTAt                       <  1:10938/141‑1 (MQ=255)

                                                                                                                  cAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTAt                       <  1:697618/141‑1 (MQ=255)

                                                                                                                  cAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTAt                       <  1:896365/141‑1 (MQ=255)

                                                                                                                   aaaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCGTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATATCCATATGCTTCACCATTGTc                      >  2:859380/1‑141 (MQ=255)

                                                                                                                    aaGCCAATCCCCATTGAATAAGTTACAGGACAGCTAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                     <  2:523489/141‑1 (MQ=255)

                                                                                                                    aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                     <  1:243793/141‑1 (MQ=255)

                                                                                                                    aaGCCAACCCCCATTGAATAAGTTACACGACAGCCAATGCTAAAGCCCCGCGCTTCAGGTCCTACAACGATATCAATATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                     <  2:373789/141‑1 (MQ=255)

                                                                                                                     aGCCAATCCCCACTGAATAAGTTACAGGCCAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa                    <  1:530029/141‑1 (MQ=255)

                                                                                                                      gCCAATCCCCATTGAATAAGTTACCGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt                   <  2:616693/141‑1 (MQ=255)

                                                                                                                        cAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATaa                 >  1:547174/1‑141 (MQ=255)

                                                                                                                            ccccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgt             >  2:704915/1‑141 (MQ=255)

                                                                                                                              ccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTCCAATTTAATCTGTTGCGTAGCCATATGCTTAACCATTATCCATACTTgtag           >  2:430941/1‑141 (MQ=255)

                                                                                                                              ccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtag           >  2:46536/1‑141 (MQ=255)

                                                                                                                                   gAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAata      <  2:178963/141‑1 (MQ=255)

                                                                                                                                   aaaTAAGTTACAGGACAGCCAAGGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAata      <  1:252662/140‑1 (MQ=255)

                                                                                                                                    aaTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAatat     >  1:928614/1‑141 (MQ=255)

                                                                                                                                       aaGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCt  <  2:775846/141‑1 (MQ=255)

GTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCT  >  USA300TCH1516_ALE/1744335‑1744610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A