Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F21 I1 R1	10	111.7	2616522	94.1%	2462147	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,544	T→C	Y43C (TAC→TGC)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,544	0	T	C	97.2%	369.6 / 0.4	106	Y43C (TAC→TGC)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (60/43); total (61/45)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 106 total aligned reads displayed.

ATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTC  >  USA300TCH1516_ALE/1744409‑1744680

aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCt                                                                                                                                     <  1:590858/141‑1 (MQ=255)

aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCt                                                                                                                                     >  4:101764/1‑141 (MQ=255)

   aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACa                                                                                                                                  >  2:402704/1‑141 (MQ=255)

     ttCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATAGCAACATCTCTGTCTTTTGCGCATTCTACAAt                                                                                                                                <  2:492572/141‑1 (MQ=255)

        aCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAAtttt                                                                                                                             >  1:112974/1‑141 (MQ=255)

              tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAAtt                                                                                                                               <  3:321215/133‑1 (MQ=255)

              tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAAtt                                                                                                                               >  4:321215/1‑133 (MQ=255)

              tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGt                                                                                                                       <  2:456967/141‑1 (MQ=255)

              tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGt                                                                                                                       >  2:37228/1‑141 (MQ=255)

                aTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTg                                                                                                                     >  2:44308/1‑141 (MQ=255)

                    cccTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATa                                                                                                                 <  3:268786/141‑1 (MQ=255)

                        tcttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCa                                                                                                             >  2:694373/1‑141 (MQ=255)

                         cttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCat                                                                                                            >  1:573770/1‑141 (MQ=255)

                          ttttCTAATAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCACGTCCTACAACGATATCAACATCTCTGTCTTTCGCGCATTCTACAATTTTATCTGTTGCATAGCCata                                                                                                           <  1:786135/141‑1 (MQ=255)

                           tttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCatat                                                                                                          <  1:338997/141‑1 (MQ=255)

                           tttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCatat                                                                                                          <  1:400696/141‑1 (MQ=255)

                              cTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCt                                                                                                       <  1:861817/141‑1 (MQ=255)

                              cTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCt                                                                                                       <  1:246583/141‑1 (MQ=255)

                               tAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCtt                                                                                                      <  3:381908/141‑1 (MQ=255)

                                aaCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTACAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                                                                                                     >  2:266517/1‑141 (MQ=255)

                                   aGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc                                                                                                  >  3:317551/1‑141 (MQ=255)

                                    ggTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCa                                                                                                 >  1:454003/1‑141 (MQ=255)

                                       gCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTa                                                                                              >  1:841813/1‑141 (MQ=255)

                                       gCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTa                                                                                              <  4:45142/141‑1 (MQ=255)

                                        cAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTAt                                                                                             >  1:118188/1‑141 (MQ=255)

                                         aaaGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATGGCCATATGCTTCACCATTATc                                                                                            >  1:538852/1‑141 (MQ=255)

                                         aaaGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCCTTATc                                                                                            >  2:271594/1‑141 (MQ=255)

                                          aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                                                                                           <  2:386450/141‑1 (MQ=255)

                                          aaGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                                                                                           <  3:133890/141‑1 (MQ=255)

                                          aaGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                                                                                           <  2:465384/141‑1 (MQ=255)

                                          aaGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                                                                                           <  3:82282/141‑1 (MQ=255)

                                            gCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt                                                                                         <  1:413986/141‑1 (MQ=255)

                                             ccAATCCCCATTGAATAAGCTACAGGACAGCCAATGATCAAGCCACGCGCTTCAGGTCCTAAAACGCTCTAAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATa                                                                                        <  1:269474/141‑1 (MQ=255)

                                                aTCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAtt                                                                                     >  2:817935/1‑141 (MQ=255)

                                                 tCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTg                                                                                    >  1:435889/1‑141 (MQ=255)

                                                     cATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtagt                                                                                <  2:314361/141‑1 (MQ=255)

                                                      aTTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtagta                                                                               >  3:146977/1‑141 (MQ=255)

                                                       ttGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTaa                                                                              <  1:497309/141‑1 (MQ=255)

                                                        tGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAat                                                                             >  1:561783/1‑141 (MQ=255)

                                                        tGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAat                                                                             >  2:98093/1‑141 (MQ=255)

                                                        tGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAat                                                                             >  1:198192/1‑141 (MQ=255)

                                                         gAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAata                                                                            <  1:609712/141‑1 (MQ=255)

                                                            tAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACGACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATcc                                                                         >  1:330101/1‑141 (MQ=255)

                                                              aGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCtt                                                                       >  1:333990/1‑141 (MQ=255)

                                                              aGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCtt                                                                       >  4:15230/1‑141 (MQ=255)

                                                               gCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTg                                                                      >  2:373010/1‑141 (MQ=255)

                                                                  aCAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGaaa                                                                   <  1:203212/141‑1 (MQ=255)

                                                                   cAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAAc                                                                  >  4:13590/1‑141 (MQ=255)

                                                                        cAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAaca                                                             >  2:515493/1‑141 (MQ=255)

                                                                         aGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAacac                                                            >  1:646686/1‑141 (MQ=255)

                                                                          gCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAACATCCTTGAAACTAACAcc                                                           <  2:515203/141‑1 (MQ=255)

                                                                           ccAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCt                                                          >  1:652212/1‑141 (MQ=255)

                                                                           ccAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCAATAATTGTAGTAATATCCTTGAAACTAACACCt                                                          <  4:195943/141‑1 (MQ=255)

                                                                           ccAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATATCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCt                                                          >  3:320475/1‑141 (MQ=255)

                                                                               tGATAAAGCCACGCGCTTCAGGTCCTACAACGTTATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTgggt                                                      <  2:181975/141‑3 (MQ=255)

                                                                               tGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGtt                                                      <  1:819653/141‑1 (MQ=255)

                                                                               tGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGtt                                                      >  4:229648/1‑141 (MQ=255)

                                                                                gATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGttt                                                     <  1:661467/141‑1 (MQ=255)

                                                                                  tAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAAttt                                                                                                                              >  2:874417/1‑66 (MQ=255)

                                                                                  tAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAAttt                                                                                                                              <  1:874417/66‑1 (MQ=255)

                                                                                  tAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCg                                                   >  2:316035/1‑141 (MQ=255)

                                                                                  tAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCg                                                   >  4:293393/1‑141 (MQ=255)

                                                                                  tAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCAATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCg                                                   >  2:611175/1‑141 (MQ=255)

                                                                                   aaaGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCgg                                                  <  4:277258/141‑1 (MQ=255)

                                                                                   aaaGCCACGCGCCTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTTTCCATAATTGTAGTAATATCCTTTACACTAACACCTGGTTTCgg                                                  <  4:36747/141‑1 (MQ=255)

                                                                                    aaGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGc                                                 >  2:853115/1‑141 (MQ=255)

                                                                                     aGCCACGCGCTTCATGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGcc                                                <  1:99329/141‑1 (MQ=255)

                                                                                     aGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTCACACCTGGTTTCGGcc                                                >  3:136042/1‑141 (MQ=255)

                                                                                     aGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGcc                                                <  1:116022/141‑1 (MQ=255)

                                                                                      gCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACATTTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCa                                               >  3:182222/1‑141 (MQ=255)

                                                                                      gCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCa                                               >  4:313631/1‑141 (MQ=255)

                                                                                            cgcTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTg                                         >  2:799024/1‑141 (MQ=255)

                                                                                            cgcTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTg                                         >  1:325289/1‑141 (MQ=255)

                                                                                            cgcTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACAACTGGTTTCGGCCAATCTTg                                         >  1:652375/1‑141 (MQ=255)

                                                                                              cTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGaa                                       >  2:620551/1‑141 (MQ=255)

                                                                                               ttCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAc                                      >  1:344191/1‑141 (MQ=255)

                                                                                                    gTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTg                                 >  2:654428/1‑141 (MQ=255)

                                                                                                    gTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTg                                 >  1:860134/1‑141 (MQ=255)

                                                                                                        tACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATAc                             <  1:551563/141‑1 (MQ=255)

                                                                                                        tACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTAGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATAc                             >  2:373638/1‑141 (MQ=255)

                                                                                                         aCAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTAGCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACg                            <  1:348011/141‑1 (MQ=255)

                                                                                                          cAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGt                           >  1:573477/1‑141 (MQ=255)

                                                                                                            aCGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTAt                         <  2:907675/141‑1 (MQ=255)

                                                                                                               atatCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGc                      <  3:180442/141‑1 (MQ=255)

                                                                                                                 atCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACCCCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCtt                    >  1:469993/1‑141 (MQ=255)

                                                                                                                 atCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCtt                    >  4:111623/1‑141 (MQ=255)

                                                                                                                    aaCATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTACTGCTTTaa                 <  2:411489/141‑1 (MQ=255)

                                                                                                                         ctctGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCa            >  3:233411/1‑141 (MQ=255)

                                                                                                                          tctGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCAt           <  2:344191/141‑1 (MQ=255)

                                                                                                                          tctGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCAt           <  2:118188/141‑1 (MQ=255)

                                                                                                                          tctGTCTTTTGCGCAGTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCAt           <  1:758030/141‑1 (MQ=255)

                                                                                                                            tGTCTTTTGTGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTa         >  4:299564/1‑141 (MQ=255)

                                                                                                                              tCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAat       >  1:485900/1‑141 (MQ=255)

                                                                                                                              tCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAat       >  1:911627/1‑141 (MQ=255)

                                                                                                                               cTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAata      <  2:91150/141‑1 (MQ=255)

                                                                                                                                ttttGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAatat     >  4:216732/1‑141 (MQ=255)

                                                                                                                                 tttGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATAtt    <  1:98093/141‑1 (MQ=255)

                                                                                                                                 tttGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATAtt    >  1:653828/1‑141 (MQ=255)

                                                                                                                                 tttGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATAtt    <  1:659488/141‑1 (MQ=255)

                                                                                                                                  ttGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATAttt   <  1:37228/141‑1 (MQ=255)

ATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTC  >  USA300TCH1516_ALE/1744409‑1744680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A