Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F52 I1 R1	27	78.9	1851050	96.3%	1782561	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,222,687	G→A	G296D (GGT→GAT)	coaBC →	Coenzyme A biosynthesis bifunctional protein CoaBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,222,687	0	G	A	98.9%	296.6 / ‑4.1	89	G296D (GGT→GAT)	coaBC	Coenzyme A biosynthesis bifunctional protein CoaBC
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (49/39); total (50/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

TCATGTTCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGGTGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACGCAAG  >  USA300TCH1516_ALE/1222550‑1222823

tcaattacAAAGTGTTGAAGAAATGTTTGAACAAGTGATAAGCCGATTAAACGAAAAAAACATTGTTGTAAAAGCAGCAGACGAATAAAAAAAAAAAACAATAAATATATAAAAACATAAAATAAAAAAGCAGGAtgatga                                                                                                                                       <  2:896385/134‑1 (MQ=255)

tCATGTTCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGAtgatga                                                                                                                                       <  2:451144/141‑1 (MQ=255)

tCATGTTCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGAtgatga                                                                                                                                       >  2:375700/1‑141 (MQ=255)

gcATGTCTAAAGCGCTGAAGAAATGTCTGAACAAGTGACAAGCTGATTTGACGATCAAGACATTGTTGTCAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGAtgatga                                                                                                                                       <  1:881989/140‑1 (MQ=255)

 cATGTTCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGgatgatgat                                                                                                                                      >  1:899733/1‑141 (MQ=255)

  aTGTTCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATAGACCGGTTGATGTATTAGAACATAAGATGAAAAAGTAGGATGATGAtt                                                                                                                                     >  2:492603/1‑141 (MQ=255)

  aTGTTCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGAtt                                                                                                                                     >  2:832335/1‑141 (MQ=255)

    gTTCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATTTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTg                                                                                                                                   <  2:4446/141‑1 (MQ=255)

      tCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTc                                                                                                                                 <  1:750897/141‑1 (MQ=255)

       cAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCa                                                                                                                                <  1:892545/141‑1 (MQ=255)

       aaaaGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCa                                                                                                                                <  2:746856/140‑1 (MQ=255)

         aaGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGt                                                                                                                              >  1:252448/1‑141 (MQ=255)

          aGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTCTTAAAACATAAAATAAATAAGCAGGATGATGGTGTGTAAGtt                                                                                                                             >  2:676464/1‑140 (MQ=255)

          aGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGAAGGACGATGATTAGTCAGTa                                                                                                                             >  2:272142/1‑141 (MQ=255)

          aGTGCAGAAGAAATGTTTGAACAAGGGACAAGCCGATTTGAAGAACAAGATATTGTGGTAAAAGCAGCAGACGTATCTGATTTGACTCCTGTGGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTa                                                                                                                             <  2:373958/141‑1 (MQ=255)

             gCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCt                                                                                                                          <  1:108191/141‑1 (MQ=255)

             gCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCt                                                                                                                          <  1:847872/141‑1 (MQ=255)

              gtGAAGAAATGTTTGAACAAGTGACAAGCCGATTGGACGAAAAAGACATTGTTGTAAACGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAAAAAAAAAAAAAAAAGCAGGATGATGATTTGTCAGTATCtt                                                                                                                         <  2:754914/140‑1 (MQ=255)

              cTGAAGAAATGTTTGAACAAGGGACAAGCCGATTTGACGAACAAGAGATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCtt                                                                                                                         <  1:118481/141‑1 (MQ=255)

               tGAAGAAATGTCTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACTCCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCttt                                                                                                                        <  1:107757/141‑1 (MQ=255)

                gaagaaATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGTATGATGATTTGTCAGTATCtttt                                                                                                                       <  2:264128/141‑1 (MQ=255)

                    aaaTGTTTGAACAAGTGACAAGCCAATTTGACCAACAAGATTTTGTTGTAAAAGCAGCAGCTGTATCTGACTATACACCGGTTGATCTATTAGAACATAAGATGAAAAAGCACGATGATGATTTGTCAGTATCTTTTAAAc                                                                                                                   <  1:595533/141‑1 (MQ=255)

                     aaTGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACg                                                                                                                  >  2:923777/1‑141 (MQ=255)

                         tttGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGAACt                                                                                                              >  2:62685/1‑141 (MQ=255)

                           tGAACAAGTGCCAAGCCTATTTGACGAACAAGATACTGTTGTAAAAGCAGCAGCCGTACCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTGGTCAGTATTTTTTAAACGTACTaa                                                                                                            <  1:211377/141‑1 (MQ=255)

                           tGAACAAGTGCCAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTaa                                                                                                            <  1:393430/141‑1 (MQ=255)

                            gAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTaaa                                                                                                           <  2:380991/141‑1 (MQ=255)

                               cAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGAc                                                                                                        <  1:29803/141‑1 (MQ=255)

                               cAAGGGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGAGGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGag                                                                                                        >  2:559698/1‑140 (MQ=255)

                                     acacGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGGCAGTATCTTTTAAACGTACTAAAGACATACtt                                                                                                  >  2:246762/1‑141 (MQ=255)

                                     aCAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCtt                                                                                                  >  2:795896/1‑141 (MQ=255)

                                      cAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTa                                                                                                 <  2:672561/141‑1 (MQ=255)

                                        aGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTAGCTTTTAAACGTACTAAAGACATTCTTaaa                                                                                               >  2:272343/1‑141 (MQ=255)

                                         gcagATTAGACGAGCAAGATAGTGTTGTAAAAGCAGCAGCCGTATCTGAATATGCACCCGTTGATGTTGTAGAAAAAAAAAAAAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAat                                                                                              <  2:790955/138‑1 (MQ=255)

                                         gCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAat                                                                                              <  1:844592/141‑1 (MQ=255)

                                           ctaTTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAatat                                                                                            <  1:179120/139‑1 (MQ=255)

                                           ccaTTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAatat                                                                                            <  1:376144/139‑1 (MQ=255)

                                           cGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAatat                                                                                            >  2:246504/1‑141 (MQ=255)

                                           cGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAatat                                                                                            <  1:500495/141‑1 (MQ=255)

                                           cGACTTGACAAACAAGATATTGTCGTAAAAGCAGCAGCCGTAGCTGACGATACACCGGGTGATGTATTAGCACATAAGATGAAAAAGCAGGATGATGATTGGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAatat                                                                                            <  1:492603/141‑1 (MQ=255)

                                            gATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATAtt                                                                                           >  1:72026/1‑141 (MQ=255)

                                            gATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATAtt                                                                                           >  2:723726/1‑141 (MQ=255)

                                             aTTTGACGAACCAGCAATTGATGTAAAAGCAGCACCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATAttt                                                                                          <  2:160208/141‑1 (MQ=255)

                                              tttGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACCCCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTa                                                                                         >  1:17552/1‑141 (MQ=255)

                                                tGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAgg                                                                                       >  1:657143/1‑141 (MQ=255)

                                                tGACGAACAAGATACTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAgg                                                                                       <  1:640935/141‑1 (MQ=255)

                                                  aCGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTCTACCCAGGTTGAGGTATGAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTg                                                                                     >  2:886513/1‑141 (MQ=255)

                                                         aGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATaa                                                                              <  1:67049/141‑1 (MQ=255)

                                                                gttgtAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATATTTTAATTGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATca                                                                       <  1:178125/141‑1 (MQ=255)

                                                                        agcagaagcCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTGTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTAttt                                                               <  2:252027/141‑1 (MQ=255)

                                                                         gcagcagcCGTATCTGACTTTACCCCGGTTGATGTATTAGAACATAAGATGAAACAGCAGGGTGACGATTTGTGAGTATAGGTTAAACGTACTAAAGACATTCTTAAATATTGAGGTGAACATAAAACATGACGGTAGTTa                                                              >  2:555205/1‑141 (MQ=255)

                                                                         gcagcagcCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATATTTTAAACGTACTACAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTa                                                              >  2:37842/1‑141 (MQ=255)

                                                                             cagcCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAg                                                          <  1:324375/141‑1 (MQ=255)

                                                                             cagcCGTATCTGACGATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAg                                                          <  1:432029/141‑1 (MQ=255)

                                                                              agcCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAgg                                                         <  2:702216/141‑1 (MQ=255)

                                                                              agcCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAgg                                                         <  1:425712/141‑1 (MQ=255)

                                                                                ccGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCt                                                       >  1:322025/1‑141 (MQ=255)

                                                                                 cGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCCCAGTATTTAATAGGCtt                                                      >  1:806832/1‑141 (MQ=255)

                                                                                 cGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGGGAACATAAAACATCCCAGTATTTAATAGGCtt                                                      >  1:806826/1‑141 (MQ=255)

                                                                                        gACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTgcagca                                               <  2:98463/141‑1 (MQ=255)

                                                                                         aCTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACCGTATTTAATAGGCTTTGcagcag                                              >  2:85091/1‑141 (MQ=255)

                                                                                              acacCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACt                                         >  1:900069/1‑141 (MQ=255)

                                                                                                 ccGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGaa                                      <  1:387152/141‑1 (MQ=255)

                                                                                                    gTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGat                                   <  2:738328/141‑1 (MQ=255)

                                                                                                     ttGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTAAATGTAATGTAAACGTACTGAAGAAAGCCGCTAGCGCCCAGTTTCACTTAAGTCATCACATTATTGAATATTCTTTGTAGCAGAGACTGAAGata                                  >  2:885594/1‑141 (MQ=255)

                                                                                                      tGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTCTTTAATAGGCTTTGCAGCAGAGACTGAAGatat                                 >  2:188308/1‑141 (MQ=255)

                                                                                                      tGATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTGTTAAGCGTACTAAAGACATTCTTAACTATTTAGGTGAACATAAAAAATGACAGTATTTAATAGGCTTTGCAGCTGAGACTGAAGgtag                                 >  2:3497/1‑140 (MQ=255)

                                                                                                       gATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGaacgtctt                                >  1:309029/1‑135 (MQ=255)

                                                                                                       gATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATAtt                                >  2:917560/1‑141 (MQ=255)

                                                                                                       gATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATAtt                                >  1:698480/1‑141 (MQ=255)

                                                                                                       gATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATAtt                                >  1:698561/1‑141 (MQ=255)

                                                                                                       gATGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATAtt                                <  1:723726/141‑1 (MQ=255)

                                                                                                        aTGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTGAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGGTATTg                               >  2:685759/1‑141 (MQ=255)

                                                                                                        aTGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTg                               >  1:180864/1‑141 (MQ=255)

                                                                                                        aTGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTg                               >  1:539196/1‑141 (MQ=255)

                                                                                                         tGTATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGa                              >  1:581392/1‑141 (MQ=255)

                                                                                                           tATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGACACTAAAACATCACAGTATTTAATAGCCTTTGCAGCAGAGACTGAAGCTATTGaaa                            >  2:506089/1‑141 (MQ=255)

                                                                                                           tATTAGAACATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCCCAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGaaa                            >  2:136843/1‑141 (MQ=255)

                                                                                                                  aCATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGc                     >  2:437980/1‑141 (MQ=255)

                                                                                                                  aCATAAGATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACGGAAGATATTGAAAATTATGc                     >  1:600644/1‑141 (MQ=255)

                                                                                                                    aTAAGATAAAAAAGCAGGATGGTGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTCTTTAATAGGCTTTGCAGCAGAGACTGAAGAAATTGACAATTATGcac                   >  1:582296/1‑141 (MQ=255)

                                                                                                                        gATGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTAAGCacaaca               >  2:290919/1‑141 (MQ=255)

                                                                                                                         aTGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGACGATCTTGAAAATTATGCAcaacaa              >  2:11927/1‑141 (MQ=255)

                                                                                                                          tGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAGCaaa             >  2:245098/1‑141 (MQ=255)

                                                                                                                          tGAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACaaa             >  1:163786/1‑141 (MQ=255)

                                                                                                                          ggAAAAAGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACaaa             <  2:252448/140‑1 (MQ=255)

                                                                                                                                aGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGCTATTGAAAATTATGCACAAAAAAAATTaa       >  2:799072/1‑140 (MQ=255)

                                                                                                                                aGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTaa       >  1:806076/1‑140 (MQ=255)

                                                                                                                                aGCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTAc       >  2:867364/1‑141 (MQ=255)

                                                                                                                                 gCAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACg      >  2:613510/1‑141 (MQ=255)

                                                                                                                                  cAGGATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATGATGCACAACAAAAATTACGc     >  1:877463/1‑141 (MQ=255)

                                                                                                                                   aGGATGATGATTTGTCAGTATCCTTTAAACGTACTCAAGACCTTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCGTTGTGGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACGCa    <  1:307118/141‑1 (MQ=255)

                                                                                                                                    ggATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTGTTTCATAGTCATGGCAGTAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACGCaa   >  1:255862/1‑141 (MQ=255)

                                                                                                                                    ggATGATGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACGCaa   <  1:917560/141‑1 (MQ=255)

                                                                                                                                     gatgatgatTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACGCAAg  >  1:876638/1‑141 (MQ=255)

TCATGTTCAAAGTGCTGAAGAAATGTTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTATCTGACTATACACCGGTTGATGTATTAGAACATAAGATGAAAAAGCAGGATGGTGATTTGTCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCACAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAATTACGCAAG  >  USA300TCH1516_ALE/1222550‑1222823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A