Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F2 I1 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
Exported |
608,922 |
C→G |
26.4% |
L444L (CTG→CTC) |
pflB ← |
pyruvate formate lyase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | Exported | 608,922 | 0 | C | G | 26.4%
| 210.4
/ 79.1
| 121 | L444L (CTG→CTC) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base C (46/43); new base G (18/14); total (64/57) |
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 |
Only 100 of 127 total aligned reads displayed.
GGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTA > Exported/608860‑608992
|
gTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtt < 1:65963/71‑1 (MQ=255)
ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGttt < 1:5431602/71‑1 (MQ=255)
ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGttt < 1:3358694/71‑1 (MQ=255)
cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt < 1:2553028/70‑1 (MQ=255)
cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTc > 1:4130640/1‑71 (MQ=255)
gACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGc > 1:1343391/1‑71 (MQ=255)
aCTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc < 1:1821597/71‑1 (MQ=255)
ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa < 1:3575453/58‑1 (MQ=255)
ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa < 1:2527513/58‑1 (MQ=255)
ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa < 1:1190732/58‑1 (MQ=255)
ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa < 1:4165177/58‑1 (MQ=255)
ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg < 1:1342268/60‑1 (MQ=255)
ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg < 1:4931906/60‑1 (MQ=255)
cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg > 1:4370299/1‑58 (MQ=255)
cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGc > 1:2451882/1‑66 (MQ=255)
cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg < 1:3010404/70‑1 (MQ=255)
cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg < 1:1832260/70‑1 (MQ=255)
cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAg < 1:808652/69‑1 (MQ=255)
cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt > 1:3961435/1‑71 (MQ=255)
cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt > 1:4920491/1‑71 (MQ=255)
cgaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcg > 1:5607572/3‑70 (MQ=255)
cAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCa > 1:2086234/1‑71 (MQ=255)
gaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgc > 1:4024147/2‑69 (MQ=255)
aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc > 1:2503781/1‑60 (MQ=255)
aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg < 1:2942887/71‑1 (MQ=255)
aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg < 1:4610772/71‑1 (MQ=255)
aCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcg < 1:62444/71‑1 (MQ=255)
cTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg > 1:2418760/1‑71 (MQ=255)
cTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg < 1:3899536/71‑1 (MQ=255)
cTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcgtg < 1:604767/71‑3 (MQ=255)
cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAc > 1:664257/1‑71 (MQ=255)
ttttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAcc > 1:3300909/1‑70 (MQ=255)
ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg < 1:3972192/38‑1 (MQ=255)
tCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg > 1:4919861/1‑68 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcgtg > 1:5664726/1‑60 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:5057062/70‑1 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:3515728/70‑1 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:5081114/70‑1 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:3217052/70‑1 (MQ=255)
aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:366141/1‑71 (MQ=255)
aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa < 1:2485728/71‑1 (MQ=255)
tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACt < 1:3820837/71‑1 (MQ=255)
ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt > 1:1781154/1‑41 (MQ=255)
ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg > 1:4708645/1‑71 (MQ=255)
ttctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTg > 1:5586913/4‑71 (MQ=255)
tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAg > 1:2557281/1‑46 (MQ=255)
tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAg > 1:42513/1‑46 (MQ=255)
tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGtt > 1:3815029/1‑49 (MQ=255)
tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg > 1:2813492/1‑70 (MQ=255)
tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg > 1:3031028/1‑70 (MQ=255)
tttCGTCAACGCCACCGTTGATTGCGTACAGCATGGTTTTCGCCAGGtt > 1:1673985/1‑49 (MQ=255)
ctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc < 1:5301396/69‑1 (MQ=255)
tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt > 1:2057723/1‑71 (MQ=255)
tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt > 1:1163239/1‑71 (MQ=255)
tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:4648764/1‑65 (MQ=255)
tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:4202879/1‑65 (MQ=255)
cGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt < 1:3273245/70‑1 (MQ=255)
tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTg < 1:5268998/71‑1 (MQ=255)
tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTg < 1:1694310/71‑1 (MQ=255)
aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt > 1:2669789/1‑71 (MQ=255)
aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt > 1:2402722/1‑71 (MQ=255)
aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt < 1:898099/71‑1 (MQ=255)
aCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACTCGCACCGAAGAACTGCATTTGtt < 1:2118390/70‑1 (MQ=255)
aCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt > 1:3459613/1‑70 (MQ=255)
ccccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa < 1:1155144/61‑1 (MQ=255)
ccccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa < 1:2393552/61‑1 (MQ=255)
cccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc > 1:1557742/2‑71 (MQ=255)
cccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc > 1:3756992/2‑71 (MQ=255)
ccgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTAc > 1:5557805/1‑70 (MQ=255)
cgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag < 1:4665827/53‑1 (MQ=255)
cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTgct > 1:1081659/1‑64 (MQ=255)
cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTgct > 1:3426410/1‑64 (MQ=255)
cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc > 1:4221099/1‑70 (MQ=255)
cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc > 1:5186119/1‑70 (MQ=255)
ccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTa > 1:5516307/1‑66 (MQ=255)
ccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCaat < 1:2375314/71‑2 (MQ=255)
cgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACg < 1:775996/71‑1 (MQ=255)
cgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACg < 1:2771617/71‑1 (MQ=255)
ttGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGat > 1:4718237/1‑71 (MQ=255)
taaTTGCTTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa < 1:438023/52‑1 (MQ=255)
tGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaa > 1:3720903/1‑46 (MQ=255)
gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:4320014/70‑1 (MQ=255)
gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:2818379/70‑1 (MQ=255)
gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:4810051/70‑1 (MQ=255)
aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:3114830/70‑1 (MQ=255)
aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:261160/70‑1 (MQ=255)
tGCGTACAGCATGGTTTTCCCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTa > 1:3366110/1‑58 (MQ=255)
gCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcca > 1:4149446/1‑38 (MQ=9)
gCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcca > 1:1466690/1‑38 (MQ=9)
gCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaagaa > 1:4326718/1‑44 (MQ=12)
gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg > 1:5340523/1‑71 (MQ=255)
gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg > 1:3865652/1‑71 (MQ=255)
catACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:2913451/3‑43 (MQ=255)
cGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:2067710/1‑43 (MQ=255)
tACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt < 1:4018357/71‑1 (MQ=255)
cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc > 1:3498844/1‑40 (MQ=255)
cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc > 1:136434/1‑40 (MQ=255)
cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa > 1:5663115/1‑71 (MQ=255)
cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa > 1:2240311/1‑71 (MQ=255)
cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa > 1:5572364/1‑71 (MQ=255)
|
GGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTA > Exported/608860‑608992
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
