Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F2 I1 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
Exported |
608,949 |
C→T |
25.2% |
A435A (GCG→GCA) |
pflB ← |
pyruvate formate lyase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | Exported | 608,949 | 0 | C | T | 25.2%
| 259.2
/ 87.3
| 147 | A435A (GCG→GCA) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base C (52/58); new base T (21/16); total (73/74) |
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 |
Only 100 of 152 total aligned reads displayed.
CTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGT > Exported/608880‑609019
|
cTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg > 1:2418760/1‑71 (MQ=255)
gCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCa > 1:2833316/1‑71 (MQ=255)
cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAc > 1:664257/1‑71 (MQ=255)
ttttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAcc > 1:3300909/1‑70 (MQ=255)
ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg > 1:55847/1‑69 (MQ=255)
tCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg > 1:4919861/1‑68 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:3217052/70‑1 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:3515728/70‑1 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:5057062/70‑1 (MQ=255)
cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:5081114/70‑1 (MQ=255)
aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:366141/1‑71 (MQ=255)
tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACt < 1:3820837/71‑1 (MQ=255)
ttctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTg > 1:5586913/4‑71 (MQ=255)
tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg > 1:3031028/1‑70 (MQ=255)
ctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc < 1:4795152/69‑1 (MQ=255)
ctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc < 1:5301396/69‑1 (MQ=255)
tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt > 1:1163239/1‑71 (MQ=255)
tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:4202879/1‑65 (MQ=255)
tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:3078734/1‑65 (MQ=255)
tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCa < 1:2517823/70‑1 (MQ=255)
cGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt > 1:462022/1‑71 (MQ=255)
tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTg < 1:5268998/71‑1 (MQ=255)
aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt > 1:2669789/1‑71 (MQ=255)
aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt < 1:898099/71‑1 (MQ=255)
aCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt > 1:3459613/1‑70 (MQ=255)
ccccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa < 1:2393552/61‑1 (MQ=255)
cccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc > 1:3756992/2‑71 (MQ=255)
ccgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTAc > 1:5557805/1‑70 (MQ=255)
cgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag < 1:4665827/53‑1 (MQ=255)
cgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag < 1:1526179/53‑1 (MQ=255)
cgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCa < 1:2065457/71‑1 (MQ=255)
cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTgct > 1:3426410/1‑64 (MQ=255)
cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTgct > 1:1081659/1‑64 (MQ=255)
cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc > 1:5186119/1‑70 (MQ=255)
cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc > 1:4221099/1‑70 (MQ=255)
ccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCaat < 1:2375314/71‑2 (MQ=255)
cgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACg < 1:2771617/71‑1 (MQ=255)
cgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACg < 1:775996/71‑1 (MQ=255)
ttGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGat > 1:4718237/1‑71 (MQ=255)
taaTTGCTTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa < 1:438023/52‑1 (MQ=255)
gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:4320014/70‑1 (MQ=255)
gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:4810051/70‑1 (MQ=255)
gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:2818379/70‑1 (MQ=255)
aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:261160/70‑1 (MQ=255)
aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:3114830/70‑1 (MQ=255)
ttGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag < 1:3908913/44‑1 (MQ=12)
gCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcca > 1:4149446/1‑38 (MQ=9)
gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg > 1:3865652/1‑71 (MQ=255)
cGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa > 1:2067710/1‑43 (MQ=255)
cGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg < 1:493001/70‑1 (MQ=255)
cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc > 1:136434/1‑40 (MQ=255)
cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc > 1:5458941/1‑40 (MQ=255)
aGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTAc < 1:1128710/71‑1 (MQ=255)
aGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTAc < 1:196137/71‑1 (MQ=255)
cATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:4089256/68‑1 (MQ=255)
cATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACg > 1:2583007/1‑70 (MQ=255)
tGGTTTTCGGCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACg < 1:4912499/68‑1 (MQ=255)
tGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcag > 1:814231/1‑71 (MQ=255)
cgTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcag > 1:1143248/2‑70 (MQ=255)
gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat < 1:2207997/56‑1 (MQ=255)
gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagc < 1:1461504/70‑1 (MQ=255)
gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagc > 1:1569553/1‑70 (MQ=255)
ttttCGCCAGGTTTGCACGCGCACCGATGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:4923260/63‑1 (MQ=255)
ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:399597/54‑1 (MQ=255)
ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:576990/54‑1 (MQ=255)
ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:3517010/54‑1 (MQ=255)
ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCtt < 1:1943784/62‑1 (MQ=255)
ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaa < 1:5109958/71‑1 (MQ=255)
tttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag > 1:5591309/1‑71 (MQ=255)
tttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag > 1:5117703/1‑71 (MQ=255)
ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg > 1:32789/1‑68 (MQ=255)
ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg > 1:5207214/1‑68 (MQ=255)
ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg > 1:5332640/1‑68 (MQ=255)
ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg > 1:5184458/1‑68 (MQ=255)
tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:3043363/70‑1 (MQ=255)
tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:202592/70‑1 (MQ=255)
tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:2032018/70‑1 (MQ=255)
tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:5547664/70‑1 (MQ=255)
tCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCagcaggc < 1:4334380/70‑1 (MQ=255)
gCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAgcaagcaa < 1:4653418/70‑1 (MQ=255)
cAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCaatc < 1:1905524/43‑3 (MQ=19)
aGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc > 1:1211034/1‑71 (MQ=255)
aGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc > 1:2480333/1‑71 (MQ=255)
ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:4240566/44‑1 (MQ=255)
ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc > 1:4642752/1‑64 (MQ=255)
ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCg < 1:224252/71‑1 (MQ=255)
ggTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCaa > 1:1476382/1‑39 (MQ=16)
gTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt < 1:4766030/51‑1 (MQ=255)
gTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGt < 1:2925223/71‑1 (MQ=255)
tttGCACGCGCACCGAAGAACTGCATTTGTTTACCAAc < 1:1089070/38‑1 (MQ=255)
tttGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat < 1:1834487/44‑1 (MQ=255)
gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:5612882/39‑1 (MQ=255)
gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:2935523/39‑1 (MQ=255)
gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg < 1:2511788/44‑1 (MQ=255)
gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:3726161/49‑1 (MQ=255)
gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACg > 1:3165380/1‑51 (MQ=255)
cACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:2138586/58‑1 (MQ=255)
aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat > 1:4914727/1‑39 (MQ=255)
aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat > 1:1657053/1‑39 (MQ=255)
aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:5496042/38‑1 (MQ=255)
|
CTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGT > Exported/608880‑609019
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
