Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F2 I1 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
Exported |
609,225 |
G→C |
21.8% |
G343G (GGC→GGG) |
pflB ← |
pyruvate formate lyase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | Exported | 609,225 | 0 | G | C | 21.8%
| 181.2
/ 42.9
| 87 | G343G (GGC→GGG) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (35/33); new base C (11/8); total (46/41) |
| Fisher's exact test for biased strand distribution p-value = 7.96e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAG > Exported/609157‑609292
|
ggccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:88760/4‑71 (MQ=255)
ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc > 1:4277363/1‑71 (MQ=255)
ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:4683451/71‑1 (MQ=255)
ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:831753/71‑1 (MQ=255)
gccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:5585024/3‑70 (MQ=255)
gccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:1969608/3‑70 (MQ=255)
cccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCAt > 1:2876034/1‑71 (MQ=255)
cATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac < 1:1498445/71‑1 (MQ=255)
cATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCAtcc < 1:28585/71‑3 (MQ=255)
aTGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac > 1:3725049/1‑70 (MQ=255)
tGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATacca > 1:1355781/1‑71 (MQ=255)
gtgtGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATcccgcc > 1:3131886/1‑71 (MQ=255)
gtgtGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATcccgc > 1:4181715/1‑68 (MQ=255)
gtgtACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATaccac > 1:1664215/1‑70 (MQ=255)
tACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGAt < 1:2213120/71‑1 (MQ=255)
cAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa > 1:1558318/1‑68 (MQ=255)
cAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATa < 1:4373535/70‑1 (MQ=255)
gggTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATAACACCGATAGAt < 1:4219930/71‑1 (MQ=255)
ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:2075826/50‑1 (MQ=255)
ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:1232033/50‑1 (MQ=255)
ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGt > 1:146097/1‑71 (MQ=255)
cAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac > 1:1681998/1‑53 (MQ=255)
aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg < 1:1815458/70‑1 (MQ=255)
aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGc < 1:3223435/71‑1 (MQ=255)
aaCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCAt > 1:180679/1‑46 (MQ=255)
aaCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAg < 1:5120824/71‑1 (MQ=255)
aCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGa < 1:1618778/71‑1 (MQ=255)
cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCg > 1:254371/1‑51 (MQ=255)
cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa > 1:1655566/1‑52 (MQ=255)
cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa > 1:3229867/1‑52 (MQ=255)
cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc > 1:2858751/1‑66 (MQ=255)
cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGAt < 1:1468218/71‑1 (MQ=255)
ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAt > 1:2928662/1‑52 (MQ=16)
ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAt > 1:3848782/1‑52 (MQ=16)
ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCAt > 1:3627170/1‑43 (MQ=255)
ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATa > 1:2470007/1‑44 (MQ=255)
ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc > 1:4980736/1‑65 (MQ=255)
aGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg < 1:2476638/70‑1 (MQ=255)
ggagTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGt < 1:2964678/68‑1 (MQ=255)
gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca > 1:5579429/1‑45 (MQ=16)
gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca > 1:417144/1‑45 (MQ=16)
gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca > 1:1750265/1‑45 (MQ=16)
gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca > 1:2007762/1‑45 (MQ=16)
gTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCgg > 1:4654558/1‑53 (MQ=255)
tttttGGTCACCAGCGTACGACCGTCCAGCCCCATcccgc < 1:2577542/40‑3 (MQ=12)
tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtc < 1:3875433/45‑2 (MQ=12)
tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTggg > 1:4588029/1‑66 (MQ=255)
tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:1174576/36‑1 (MQ=255)
tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:1735616/36‑1 (MQ=255)
tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:3161205/36‑1 (MQ=255)
tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:1202690/36‑1 (MQ=255)
tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:3431981/36‑1 (MQ=255)
tttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc < 1:151642/70‑1 (MQ=255)
ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc > 1:2138134/1‑69 (MQ=255)
ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc > 1:1515688/1‑69 (MQ=255)
tttGGTAACCAGGGTACGACCGTCGAGGCCCATacca > 1:1993855/1‑37 (MQ=255)
tttGGTAACCAGGGTACGACCGTCGAGGCCCATacc > 1:266622/1‑36 (MQ=255)
tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCg > 1:2000489/1‑62 (MQ=255)
tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc > 1:2712682/1‑69 (MQ=255)
tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc > 1:2474570/1‑69 (MQ=255)
ttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg < 1:1269378/70‑3 (MQ=255)
ggTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCagag > 1:1969865/1‑70 (MQ=255)
gtcaCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGaa < 1:4104932/68‑1 (MQ=255)
gTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAgaga < 1:2512797/70‑1 (MQ=255)
aCCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAg > 1:845085/1‑71 (MQ=255)
aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGcc < 1:765651/62‑1 (MQ=255)
aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAg < 1:3508966/71‑1 (MQ=255)
cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGtt > 1:4221212/1‑71 (MQ=255)
cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt > 1:2647042/1‑70 (MQ=255)
cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt > 1:3660695/1‑70 (MQ=255)
cAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg > 1:2796722/1‑60 (MQ=255)
ggTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCAt < 1:1293650/71‑1 (MQ=255)
tACGACCGTCGAGGCCCATACCACCGATAGATTCgg > 1:4678409/1‑36 (MQ=255)
tACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCg < 1:2079290/71‑1 (MQ=255)
tACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCg < 1:3889893/71‑1 (MQ=255)
cGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg < 1:3613668/37‑1 (MQ=255)
cGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg < 1:3607041/37‑1 (MQ=255)
aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAtt > 1:1698968/1‑71 (MQ=255)
aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAt > 1:624818/1‑70 (MQ=255)
ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc > 1:4124624/1‑71 (MQ=255)
ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc > 1:572776/1‑71 (MQ=255)
gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg < 1:2518763/42‑1 (MQ=255)
gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg < 1:1691527/71‑1 (MQ=255)
gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg < 1:850734/71‑1 (MQ=255)
tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa < 1:4986670/55‑1 (MQ=255)
tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa < 1:4247526/55‑1 (MQ=255)
tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa < 1:3899602/55‑1 (MQ=255)
tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa < 1:3307660/55‑1 (MQ=255)
tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg > 1:4205076/1‑70 (MQ=255)
aGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAg > 1:4514107/1‑70 (MQ=255)
|
GGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAG > Exported/609157‑609292
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
