Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F2 I1 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
Exported |
833,966 |
C→T |
48.7% |
H808H (CAC→CAT) |
narG → |
nitrate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | Exported | 833,966 | 0 | C | T | 48.7%
| 5.3
/ 105.2
| 80 | H808H (CAC→CAT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (12/29); new base T (16/23); total (28/52) |
| Fisher's exact test for biased strand distribution p-value = 3.50e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01 |
CATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCA > Exported/833897‑834030
|
catTTTGCCGACGGCGACCTGGTACGAAAAAGAAGACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:2473935/1‑71 (MQ=255)
caatttGCCGACGGCGACCTGGTACGAAAAAGACTACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:1915154/4‑71 (MQ=255)
atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAccc > 1:1279436/1‑71 (MQ=255)
gCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg < 1:3934286/70‑1 (MQ=255)
cGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcg > 1:182574/1‑71 (MQ=255)
gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc < 1:4778080/71‑1 (MQ=255)
gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc < 1:2071345/71‑1 (MQ=255)
cGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg < 1:1610348/62‑1 (MQ=255)
cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTc > 1:3764463/1‑70 (MQ=255)
cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCg < 1:495935/71‑1 (MQ=255)
gTACGAAAAAGACTATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa < 1:2753154/69‑1 (MQ=255)
gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt > 1:2928721/1‑54 (MQ=255)
gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa < 1:774894/69‑1 (MQ=255)
gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATc > 1:5188932/1‑71 (MQ=255)
gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCTGTCGAt < 1:5336978/70‑1 (MQ=255)
gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt < 1:4590972/70‑1 (MQ=255)
gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt < 1:2241915/70‑1 (MQ=255)
gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt < 1:3132333/70‑1 (MQ=255)
tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCt < 1:1476946/53‑1 (MQ=255)
aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCg > 1:93619/1‑71 (MQ=255)
gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAcc < 1:533759/46‑1 (MQ=255)
aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGcc > 1:159557/1‑71 (MQ=255)
aaaaGACGATATGAACATCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt < 1:5196116/71‑3 (MQ=255)
aaaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt < 1:1165135/71‑3 (MQ=255)
aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGcc < 1:2971644/70‑1 (MQ=255)
aaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTg < 1:527492/71‑1 (MQ=255)
acgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc < 1:4214709/52‑1 (MQ=255)
cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt > 1:796807/1‑42 (MQ=25)
cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt > 1:1265174/1‑42 (MQ=25)
cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt > 1:2625660/1‑42 (MQ=25)
gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg < 1:2757281/70‑1 (MQ=255)
gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg < 1:673802/70‑1 (MQ=255)
gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg < 1:5605469/70‑1 (MQ=255)
ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcgg < 1:4542792/46‑1 (MQ=255)
ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcgg < 1:3226024/46‑1 (MQ=255)
ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcgg < 1:4017036/46‑1 (MQ=255)
ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcgg < 1:3953829/46‑1 (MQ=255)
acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa < 1:295201/55‑1 (MQ=255)
cATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg > 1:4451928/1‑40 (MQ=255)
aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcggcg > 1:1377363/1‑48 (MQ=255)
aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcggcg > 1:761349/1‑48 (MQ=255)
aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcagcg > 1:1760693/1‑48 (MQ=255)
aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCg < 1:52347/52‑1 (MQ=37)
aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa < 1:3625764/53‑1 (MQ=37)
tGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATc < 1:2116227/54‑1 (MQ=255)
tGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGa < 1:2070046/69‑1 (MQ=255)
gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGaa > 1:4454508/1‑69 (MQ=255)
aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg > 1:1726496/1‑64 (MQ=255)
aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:3035158/71‑1 (MQ=255)
cTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa < 1:2661546/46‑1 (MQ=255)
cTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa < 1:317352/46‑1 (MQ=255)
cTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa < 1:642867/46‑1 (MQ=255)
ctCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGaa < 1:2503919/57‑1 (MQ=255)
ctCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCAGCGTGGGaa < 1:2231905/57‑1 (MQ=255)
tCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATc > 1:3815887/1‑46 (MQ=255)
cGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTgg > 1:5187583/1‑71 (MQ=255)
ggATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTggg < 1:1846389/71‑1 (MQ=255)
ggATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTgg > 1:3689816/1‑70 (MQ=255)
tatGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGTGaaa < 1:1481207/71‑1 (MQ=255)
tatGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGaaa < 1:2915590/71‑1 (MQ=255)
tatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGa < 1:3322576/69‑1 (MQ=255)
tGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCgg < 1:3744128/42‑1 (MQ=38)
tGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTggg < 1:2517544/48‑1 (MQ=37)
tGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTggg < 1:4323523/48‑1 (MQ=37)
tGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTggg < 1:1551834/48‑1 (MQ=37)
tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGc < 1:2124322/52‑1 (MQ=255)
gCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGa > 1:2439283/1‑60 (MQ=255)
cATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCt < 1:3401193/70‑1 (MQ=255)
cATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTa < 1:4377260/71‑1 (MQ=255)
cATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTa < 1:771430/71‑1 (MQ=255)
tCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:78578/55‑1 (MQ=255)
cGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACaaa < 1:1469837/71‑1 (MQ=255)
cGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACa > 1:4287315/1‑69 (MQ=255)
cGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCt > 1:2225820/1‑39 (MQ=255)
cGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACa > 1:3522696/1‑69 (MQ=255)
gTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAAt < 1:4112380/63‑1 (MQ=255)
tttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGa < 1:2210751/46‑1 (MQ=255)
tttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:5515961/51‑1 (MQ=255)
ttATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAgg > 1:4407565/1‑69 (MQ=255)
tATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTgg > 1:5665279/1‑55 (MQ=255)
tATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTgg > 1:5369519/1‑55 (MQ=255)
tATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAggta > 1:4662936/1‑68 (MQ=255)
tATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCa > 1:3288003/1‑71 (MQ=255)
tATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCa > 1:5064259/1‑71 (MQ=255)
|
CATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCA > Exported/833897‑834030
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
