Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
793,323 |
A→G |
G296G (GGT→GGC) |
modF ← |
fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 793,323 | 0 | A | G | 100.0%
| 18.1
/ NA
| 9 | G296G (GGT→GGC) | modF | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/9); total (0/9) |
CCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGC > W3110S.gb/793295‑793332
|
tcAGGCTTAATAACGTCTATTTTCCCGCGCCATTTGGc < 1:1271804/37‑1 (MQ=25)
tcAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGc < 1:225317/37‑1 (MQ=25)
tcAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGc < 1:229864/37‑1 (MQ=25)
tcAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGc < 1:330364/37‑1 (MQ=25)
tcAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGc < 1:513181/37‑1 (MQ=25)
tcAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGc < 1:565238/37‑1 (MQ=25)
tcAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGc < 1:901051/37‑1 (MQ=25)
tcAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGc < 1:957947/37‑1 (MQ=25)
tcAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGc < 1:964885/37‑1 (MQ=25)
|
CCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGC > W3110S.gb/793295‑793332
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
