Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,151,694 T→C T477A (ACG→GCG)  yegI ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,151,6940TC100.0% 376.3 / NA 129T477A (ACG→GCG) yegIconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (129/0);  total (129/0)

Only 100 of 129 total aligned reads displayed.

TTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGA  >  W3110S.gb/2151682‑2151744
            |                                                  
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGcccgccca                    >  1:1212522/1‑43 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATc         >  1:461807/1‑56 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTggatg   >  1:566074/1‑59 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:41773/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:246036/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:554877/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:517966/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:514083/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:509400/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:480325/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:478157/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:466499/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:283053/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:436917/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:282298/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:271623/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:399519/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:39574/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:377804/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:372711/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:347081/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:337568/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:294673/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1027791/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:696101/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:962675/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:961894/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:959798/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:856770/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:856709/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:789727/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:788203/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:777271/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:747973/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:733548/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:565231/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:689806/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:678320/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:659713/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:658113/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:656217/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:65464/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:640262/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:633760/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:629803/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:580534/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1251603/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1453025/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1416903/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:134457/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:2368/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1320876/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1317599/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1314661/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:128334/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1270638/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1255189/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1339896/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1236762/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1231637/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:120994/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1195163/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1193689/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1127306/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1109123/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1047952/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1045998/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1034065/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1574475/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:22680/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:207948/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:190186/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1622126/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1576047/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1546367/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1528695/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1521047/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1460388/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1486111/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1482915/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGctg   >  1:1482523/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:415171/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:208300/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:1391242/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:901825/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:1087272/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:1357244/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:77511/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:342806/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:456899/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:349882/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:1453812/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:671647/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:1239071/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:380157/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCTGGct    >  1:128336/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCCGCCCAAGTGTTGGATCAGGctg   >  1:114037/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCCAGCCCAAGTGTTGGATCTGGct    >  1:741347/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCTTCCAGCACGCCCAAGTGTTGGATCTGGc     >  1:1138069/1‑60 (MQ=255)
tttCGAGCATCGCCCGTTTGGCGATAAAACCATcc                              >  1:1645875/1‑35 (MQ=37)
            |                                                  
TTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGA  >  W3110S.gb/2151682‑2151744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A