Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,219,995 A→G S249S (AGT→AGC)  yehX ← predicted transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,219,9950AG100.0% 619.9 / NA 210S249S (AGT→AGC) yehXpredicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (210/0);  total (210/0)

Only 100 of 210 total aligned reads displayed.

CGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTAC  >  W3110S.gb/2219975‑2220032
                    |                                     
cgacgaACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:618802/1‑57 (MQ=255)
cgACGCAGGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1537665/1‑57 (MQ=255)
cgACGCACTTAATCCGCCACGCTACGTAACGAAAg                         >  1:859803/1‑35 (MQ=38)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGTAGGCGGACACCCAGTTCACTat  >  1:357745/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGgcgga                 >  1:12817/1‑41 (MQ=38)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGgcg                   >  1:10966/1‑41 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACCCCCAGTTCACTat  >  1:531741/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGt         >  1:1324820/1‑51 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACt    >  1:302112/1‑56 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:386266/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:548059/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:506743/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:497788/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:495985/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:46907/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:439727/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:431065/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:400928/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:274187/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:364482/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:336901/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:31566/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:313956/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:294188/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:285774/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:100002/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:28492/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:848674/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:984377/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:966932/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:927765/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:92628/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:924729/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:913672/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:906661/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:903851/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:871092/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:856497/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:571804/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:837813/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:829954/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:741571/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:722202/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:710978/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:675514/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:657935/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:656062/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:632037/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:580146/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1102061/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1354368/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:251064/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1313861/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:13007/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1275794/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:122720/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:121508/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1199718/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1190376/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1153538/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1142447/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1347913/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1099306/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1088350/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1072763/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1052160/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1036845/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1034623/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1026981/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1014432/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1011965/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1003209/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1579256/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:220524/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:188898/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:171142/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1635945/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1616193/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1602917/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1596527/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1371189/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1561827/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1557557/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:154973/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:140540/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1435329/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1465425/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1482300/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1486378/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1526060/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTa   >  1:861084/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTa   >  1:349300/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTa   >  1:880851/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTa   >  1:1604646/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTa   >  1:1509542/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTa   >  1:453845/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTa   >  1:817506/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTAACTat  >  1:6435/1‑57 (MQ=255)
cgACGCACGTAATCCGCCACGCTACGAAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:329482/1‑57 (MQ=255)
cgACGCACGTAATCAGCCACGCTACGTAACGAAAGCAGGCGGACACCCAGTTCACTat  >  1:1020104/1‑57 (MQ=255)
                    |                                     
CGACGCACGTAATCCGCCACACTACGTAACGAAAGCAGGCGCACACCCAGTTCACTAC  >  W3110S.gb/2219975‑2220032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A