Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
821,445 |
T→C |
75.0% |
S7G (AGC→GGC) |
ldrA ← |
toxic polypeptide, small |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 821,445 | 0 | T | C | 75.0%
| 67.3
/ 22.4
| 44 | S7G (AGC→GGC) | ldrA | toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (2/9); new base C (18/15); total (20/24) |
| Fisher's exact test for biased strand distribution p-value = 4.45e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTA > minE/821395‑821506
|
cGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGt > 1:1416822/1‑71 (MQ=255)
cGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGt > 1:4303630/1‑71 (MQ=255)
gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc < 1:4339373/71‑1 (MQ=255)
gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc < 1:1710798/71‑1 (MQ=255)
gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc < 1:2501277/71‑1 (MQ=255)
ccaccaGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCa < 1:3524084/71‑1 (MQ=34)
caccaGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAt > 1:1216590/1‑71 (MQ=255)
accaGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATg > 1:4371520/1‑71 (MQ=255)
ccaGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATgg > 1:5172359/1‑71 (MQ=34)
aGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCa < 1:3448904/71‑1 (MQ=34)
aGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGc > 1:647017/1‑70 (MQ=255)
aGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCa < 1:4313762/71‑1 (MQ=255)
cTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCaa < 1:1627951/70‑1 (MQ=35)
cGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgcgc > 1:241570/1‑69 (MQ=25)
gCTGCGGTAATAATTCCCGCCAGGATCGTTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAg > 1:2195765/1‑71 (MQ=255)
gCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCa > 1:2356212/1‑55 (MQ=35)
gCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGa < 1:2454213/70‑1 (MQ=25)
gCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACt > 1:5638646/1‑61 (MQ=25)
cGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTc < 1:4204334/71‑1 (MQ=34)
cGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCg < 1:3076164/69‑1 (MQ=255)
ggTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa < 1:4023875/71‑1 (MQ=255)
gTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCat < 1:5586855/71‑1 (MQ=255)
taataaTTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata < 1:1851649/71‑1 (MQ=255)
aataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata > 1:2923647/1‑70 (MQ=255)
aataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata > 1:2321353/1‑70 (MQ=255)
ataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata > 1:4554846/1‑69 (MQ=255)
taatTCCCGCCAGGATCGGTGCTGCCAGTTCGTGCCAGAAAGTCATGGCAAACTgc < 1:149218/56‑1 (MQ=255)
taatTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACt < 1:2683247/54‑1 (MQ=35)
taatTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgc < 1:5047611/56‑1 (MQ=35)
taatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgcgc < 1:1263092/58‑1 (MQ=255)
taatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAg > 1:55659/1‑71 (MQ=21)
ttCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATg < 1:1596578/44‑1 (MQ=255)
ttCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCaaa > 1:4809753/1‑49 (MQ=255)
cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc > 1:5463922/1‑39 (MQ=255)
cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc > 1:1615482/1‑39 (MQ=255)
cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc > 1:4940680/1‑39 (MQ=255)
ccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCaaa > 1:1809766/1‑46 (MQ=255)
gCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttg < 1:3886981/71‑1 (MQ=34)
gCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttg < 1:2042218/71‑1 (MQ=34)
cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGTCAAACTGCGCGAGCGTCa < 1:3270180/56‑1 (MQ=11)
cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa < 1:4504964/56‑1 (MQ=255)
cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa < 1:1160594/56‑1 (MQ=255)
aGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttgttg > 1:5482186/1‑71 (MQ=21)
gTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTa < 1:4394382/66‑1 (MQ=25)
|
CGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTA > minE/821395‑821506
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
