Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
821,728 |
A→G |
24.6% |
intergenic (‑265/+189) |
ldrA ← / ← ldrC |
toxic polypeptide, small/toxic polypeptide, small |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 821,728 | 0 | A | G | 24.6%
| 139.5
/ 40.4
| 73 | intergenic (‑265/+189) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base A (25/30); new base G (9/9); total (34/39) |
| Fisher's exact test for biased strand distribution p-value = 7.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 |
GGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCT > minE/821658‑821791
|
ggATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATa < 1:304988/71‑1 (MQ=255)
gATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATa > 1:2825760/1‑70 (MQ=255)
aTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAag < 1:2725243/69‑1 (MQ=255)
ggTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCgg > 1:2642846/1‑71 (MQ=39)
gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:1104757/1‑70 (MQ=40)
gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc < 1:5623243/71‑1 (MQ=39)
gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc < 1:5258918/71‑1 (MQ=39)
tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:3703521/1‑71 (MQ=39)
tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:3998554/1‑71 (MQ=39)
actacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:4528678/1‑70 (MQ=40)
ctacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAAc < 1:1392233/70‑1 (MQ=40)
cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa > 1:359793/1‑50 (MQ=255)
cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa > 1:4662579/1‑50 (MQ=255)
cAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATaaa > 1:656551/1‑40 (MQ=40)
cAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATaaa > 1:4800916/1‑40 (MQ=40)
aaTTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTg < 1:2788131/69‑1 (MQ=35)
tCATCTTCACTTCATCCCTGAAATGTTTGCAATaa > 1:3548618/1‑35 (MQ=37)
tCATCTTCACTTCATCCCTGAAATGTTTGCAATaa > 1:377284/1‑35 (MQ=37)
tCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa < 1:703983/41‑1 (MQ=38)
tCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa < 1:3211241/41‑1 (MQ=38)
cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:4463368/1‑53 (MQ=35)
cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:2354029/1‑53 (MQ=35)
cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACa > 1:3531416/1‑58 (MQ=35)
cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc > 1:636252/1‑60 (MQ=35)
aTCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa < 1:1923710/39‑1 (MQ=40)
aTCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGt < 1:3024025/71‑1 (MQ=39)
ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt > 1:3937129/1‑69 (MQ=40)
ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGt < 1:4887246/68‑1 (MQ=40)
ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGAACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt > 1:320400/1‑69 (MQ=255)
tcacttcaTCCCTGAAATGTTTGCAATAAAGAGAACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:576134/70‑1 (MQ=255)
cacttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCa > 1:12353/1‑70 (MQ=11)
cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTcc > 1:797557/1‑40 (MQ=40)
cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGttt < 1:1446410/68‑1 (MQ=40)
cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:766275/69‑1 (MQ=40)
cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:4797940/69‑1 (MQ=40)
cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:2007512/69‑1 (MQ=40)
cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCa > 1:2046808/1‑70 (MQ=255)
cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt > 1:2184858/1‑71 (MQ=255)
acttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:5603295/70‑1 (MQ=11)
acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:523176/70‑1 (MQ=255)
acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:4844769/70‑1 (MQ=255)
acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:2873495/70‑1 (MQ=255)
acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATg < 1:2383303/71‑1 (MQ=255)
acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATg < 1:216736/71‑1 (MQ=255)
cttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:5234717/67‑1 (MQ=40)
cttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGa < 1:1444423/71‑1 (MQ=255)
caTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGa > 1:1055425/1‑68 (MQ=11)
caTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTg > 1:3842299/1‑71 (MQ=14)
aTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGc < 1:1585178/71‑1 (MQ=255)
tCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:4156454/64‑1 (MQ=255)
cccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGct > 1:5006544/1‑70 (MQ=255)
ccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc > 1:83291/1‑45 (MQ=38)
ccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc > 1:5061367/1‑45 (MQ=38)
cTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:4718052/71‑1 (MQ=17)
tGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc < 1:1754790/37‑1 (MQ=40)
gAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga > 1:2975512/1‑71 (MQ=17)
gAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGctc > 1:2800009/1‑67 (MQ=255)
gAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGctc > 1:3716784/1‑67 (MQ=255)
aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:1047272/58‑1 (MQ=11)
aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat < 1:976120/71‑1 (MQ=17)
aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat < 1:2869499/71‑1 (MQ=17)
aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:5271687/1‑37 (MQ=40)
aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTgg < 1:4647999/69‑1 (MQ=255)
aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:3052047/71‑1 (MQ=255)
aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:5206207/71‑1 (MQ=255)
aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:3996150/71‑1 (MQ=255)
aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGga > 1:807401/1‑70 (MQ=255)
aaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg < 1:3749695/71‑1 (MQ=18)
tttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGc > 1:385316/1‑71 (MQ=18)
ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg > 1:4706826/1‑66 (MQ=18)
ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt > 1:1955198/1‑71 (MQ=18)
ttGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc > 1:2712986/1‑41 (MQ=38)
tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag > 1:4322892/1‑66 (MQ=18)
tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag > 1:5250076/1‑66 (MQ=18)
tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt < 1:2690654/70‑1 (MQ=18)
tGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTg > 1:4844364/1‑56 (MQ=255)
aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:1423380/58‑1 (MQ=17)
aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:4827439/58‑1 (MQ=17)
aTAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc < 1:4906049/36‑1 (MQ=40)
aaaGAGTACATTCCGGCTTTTCAACAGCTGTTGCAg > 1:2203093/1‑36 (MQ=33)
|
GGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCT > minE/821658‑821791
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
