Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
821,788 |
T→G |
45.5% |
intergenic (‑325/+129) |
ldrA ← / ← ldrC |
toxic polypeptide, small/toxic polypeptide, small |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 821,788 | 0 | T | G | 45.5%
| ‑6.2
/ 77.6
| 55 | intergenic (‑325/+129) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (13/17); new base G (10/15); total (23/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 |
TTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGT > minE/821720‑821854
|
tttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGc > 1:385316/1‑71 (MQ=18)
ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt > 1:1955198/1‑71 (MQ=18)
tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt < 1:2690654/70‑1 (MQ=18)
aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTc < 1:5614866/71‑1 (MQ=25)
gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg < 1:3616340/71‑1 (MQ=37)
gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg < 1:329877/71‑1 (MQ=37)
gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg > 1:3947259/1‑71 (MQ=37)
gagTGCATTCCGG‑TTTTTAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCggg > 1:1143634/1‑71 (MQ=255)
gtgcATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt < 1:3826935/68‑1 (MQ=37)
tACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGatgatg > 1:4154224/1‑56 (MQ=255)
aCATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTg > 1:4311177/1‑71 (MQ=255)
aTTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGcc < 1:501107/71‑1 (MQ=38)
ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg < 1:1082392/59‑1 (MQ=37)
ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg < 1:545143/59‑1 (MQ=37)
ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa < 1:2800549/70‑1 (MQ=255)
ccGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa < 1:5418685/70‑1 (MQ=255)
cGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCgg < 1:2158097/61‑1 (MQ=255)
tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt > 1:1653836/2‑60 (MQ=255)
tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAat < 1:2084324/66‑1 (MQ=255)
cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc > 1:2022357/1‑47 (MQ=255)
cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc > 1:1070892/1‑47 (MQ=255)
cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt < 1:4230168/71‑1 (MQ=255)
cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt < 1:5015339/71‑1 (MQ=255)
ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCtt < 1:4371243/48‑1 (MQ=255)
tttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAata > 1:4012282/1‑66 (MQ=255)
ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:3830683/1‑48 (MQ=255)
ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:1946119/1‑48 (MQ=255)
ttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTAc < 1:412948/70‑1 (MQ=255)
tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:2781536/1‑47 (MQ=255)
tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:723886/1‑47 (MQ=255)
tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa > 1:5660595/1‑62 (MQ=255)
tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTa < 1:3713959/68‑1 (MQ=255)
tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTACCAGCTCGGGTTGCCa > 1:2337431/1‑61 (MQ=255)
cAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg < 1:2585273/71‑1 (MQ=255)
tGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa > 1:921513/1‑69 (MQ=255)
ttGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCa < 1:5501011/51‑1 (MQ=255)
gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:1084642/70‑1 (MQ=255)
gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:3781259/70‑1 (MQ=255)
acAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa > 1:1986682/2‑65 (MQ=255)
cAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa < 1:2591327/70‑1 (MQ=255)
cAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATa < 1:5597983/70‑1 (MQ=255)
tCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTa < 1:1360273/52‑1 (MQ=255)
gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc > 1:1739819/1‑51 (MQ=255)
gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc > 1:4856740/1‑51 (MQ=255)
gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTAGGGTTGCCAATATTTAc > 1:1330868/1‑51 (MQ=255)
tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAtt > 1:4039707/1‑45 (MQ=255)
tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:2494345/70‑1 (MQ=255)
aTGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaaa < 1:4944308/70‑1 (MQ=255)
ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1054012/71‑1 (MQ=255)
ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1508998/71‑1 (MQ=255)
tctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa < 1:2090510/46‑1 (MQ=255)
tctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa < 1:5363010/46‑1 (MQ=255)
ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:1141625/59‑1 (MQ=255)
tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGAc > 1:34747/1‑55 (MQ=255)
tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:2228891/68‑1 (MQ=255)
ggATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGc > 1:2077133/1‑70 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:3632523/36‑1 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:4916776/36‑1 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:1575054/36‑1 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa > 1:4257911/1‑66 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAgg < 1:2680119/68‑1 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAgg < 1:4582793/68‑1 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGc < 1:3805761/69‑1 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt < 1:3346073/70‑1 (MQ=255)
gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:2476154/1‑71 (MQ=255)
gatgagGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCt < 1:2602855/70‑1 (MQ=255)
atgaggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGt < 1:354236/71‑1 (MQ=255)
tgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGc < 1:4883770/57‑1 (MQ=255)
|
TTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGT > minE/821720‑821854
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
