Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R1	2892	75.3	5698161	66.1%	3766484	59.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	833,939	T→C	39.6%	N799N (AAT→AAC)	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	833,939	0	T	C	39.6%	68.9 / 112.1	106	N799N (AAT→AAC)	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand): ref base T (25/39); new base C (24/18); total (49/57)
Fisher's exact test for biased strand distribution p-value = 7.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 106 total aligned reads displayed.

AGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGC  >  minE/833874‑834008

aGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTc                                                                  >  1:5319269/1‑71 (MQ=255)

 gCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg                                                                 >  1:2561185/1‑71 (MQ=255)

       gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATg                                                           >  1:5295264/1‑71 (MQ=255)

        tctcTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATAc                                                                     >  1:4163088/1‑60 (MQ=255)

            tATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCa                                                         <  1:2424132/68‑1 (MQ=255)

            tATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATcc                                                      >  1:2999031/1‑71 (MQ=255)

              ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCg                                                     <  1:3539171/70‑1 (MQ=255)

              ctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGt                                                    >  1:141150/2‑71 (MQ=255)

               tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGc                                                          >  1:3065344/1‑64 (MQ=255)

                ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt                                                   <  1:5072479/70‑1 (MQ=255)

                 cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa                                                 >  1:2054840/1‑71 (MQ=255)

                 cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa                                                 >  1:4247396/1‑71 (MQ=255)

                 cGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa                                                 >  1:2743258/1‑71 (MQ=255)

                  gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa                                                 <  1:1043388/67‑1 (MQ=255)

                  gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt                                                >  1:892632/4‑71 (MQ=255)

                  gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt                                                >  1:892630/4‑71 (MQ=255)

                  gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt                                                >  1:3568848/4‑71 (MQ=255)

                  gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt                                                >  1:3897985/4‑71 (MQ=255)

                   aCATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATa                                                                      >  1:1793678/1‑48 (MQ=255)

                   aCATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa                                                 >  1:426848/1‑69 (MQ=255)

                    catcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTc                                              <  1:5664120/71‑1 (MQ=255)

                     atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGata                                                             >  1:4274569/1‑55 (MQ=255)

                     atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGata                                                             >  1:3466423/1‑55 (MQ=255)

                       catTTTGCCGACGGCGACCTGGTACGAAAAAGAAGACATGAATACTTCGGATATGCATCCGTTTATTCAcc                                           >  1:2473935/1‑71 (MQ=255)

                       caatttGCCGACGGCGACCTGGTACGAAAAAGACTACATGAATACTTCGGATATGCATCCGTTTATTCAcc                                           >  1:1915154/4‑71 (MQ=255)

                        atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg                                                                 <  1:1370847/48‑1 (MQ=255)

                        atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa                                                               <  1:4351762/50‑1 (MQ=255)

                        atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAccc                                          >  1:1279436/1‑71 (MQ=255)

                             gCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg                                      <  1:3934286/70‑1 (MQ=255)

                                 aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata                                                             <  1:2219525/43‑1 (MQ=255)

                                  cGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcg                                >  1:182574/1‑71 (MQ=255)

                                   tgCGACGTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt                                                   <  1:5074128/50‑1 (MQ=255)

                                   ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt                                                   <  1:3505476/51‑1 (MQ=255)

                                    gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc                              <  1:2071345/71‑1 (MQ=255)

                                    gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc                              <  1:4778080/71‑1 (MQ=255)

                                     cGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg                                      <  1:1610348/62‑1 (MQ=255)

                                      gACCTGGTACGAAAAAGACGACATGAATACTTCGGata                                                             <  1:3961289/38‑1 (MQ=255)

                                      gACCTGGTACGAAAAAGACGACATGAATACTTCGGata                                                             <  1:1009580/38‑1 (MQ=255)

                                         cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGtt                                                   <  1:1961068/45‑1 (MQ=255)

                                         cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGtt                                                   <  1:3418381/45‑1 (MQ=255)

                                         cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGtt                                                   <  1:1536267/45‑1 (MQ=255)

                                         cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGtt                                                   <  1:2369790/45‑1 (MQ=255)

                                         cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTc                          >  1:3764463/1‑70 (MQ=255)

                                         cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCg                         <  1:495935/71‑1 (MQ=255)

                                          tGGTACGAAAAAGACGATATGAACACCTCGGATATGCa                                                         >  1:2451133/1‑38 (MQ=25)

                                          tGGTACGAAAAAGACGATATGAACACCTCGGATATGCa                                                         >  1:705505/1‑38 (MQ=25)

                                          tGGTACGAAAAAGACGATATGAACACCTCGGATATGCa                                                         >  1:2547601/1‑38 (MQ=25)

                                            gTACGAAAAAGACTATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa                        <  1:2753154/69‑1 (MQ=255)

                                            gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt                                     >  1:2928721/1‑54 (MQ=255)

                                            gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa                        <  1:774894/69‑1 (MQ=255)

                                            gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATc                      >  1:5188932/1‑71 (MQ=255)

                                            gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGt                                                    <  1:34185/41‑1 (MQ=255)

                                            gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCTGTCGAt                       <  1:5336978/70‑1 (MQ=255)

                                            gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt                       <  1:3132333/70‑1 (MQ=255)

                                            gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt                       <  1:2241915/70‑1 (MQ=255)

                                            gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt                       <  1:4590972/70‑1 (MQ=255)

                                             tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCt                                       <  1:1476946/53‑1 (MQ=255)

                                             tACGAAAAAGACGACATAAATACTTCGGATATGCATCCGt                                                    <  1:4233956/40‑1 (MQ=255)

                                              aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCg                    >  1:93619/1‑71 (MQ=255)

                                               cGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt                                                   <  1:5180870/39‑1 (MQ=255)

                                                gAAAAAGACGATATGAACACCTCGGATATGCATCCGtt                                                   <  1:3706483/38‑1 (MQ=37)

                                                gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa                                                 <  1:416101/40‑1 (MQ=255)

                                                gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa                                                 <  1:5105084/40‑1 (MQ=255)

                                                gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa                                                 <  1:4459474/40‑1 (MQ=255)

                                                gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAcc                                           <  1:533759/46‑1 (MQ=255)

                                                 aaaaaGACGACATGAATACTTCGGATATGCATCCGtt                                                   >  1:59931/1‑37 (MQ=255)

                                                 aaaaaGACGACATGAATACTTCGGATATGCATCCGtt                                                   >  1:2255891/1‑37 (MQ=255)

                                                 aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCa                                             <  1:1322700/43‑1 (MQ=255)

                                                 aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCa                                             <  1:1377918/43‑1 (MQ=255)

                                                 aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGcc                 >  1:159557/1‑71 (MQ=255)

                                                  aaaaGACGATATGAACATCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt                <  1:5196116/71‑3 (MQ=255)

                                                  aaaaGACGATATGAACACCTCGGATATGCATCCGTTTa                                                 >  1:3362053/1‑38 (MQ=37)

                                                  aaaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt                <  1:1165135/71‑3 (MQ=255)

                                                  aaaaGACGACATGAATACTTCGGATATGCATCCGttt                                                  >  1:772551/1‑37 (MQ=255)

                                                  aaaaGACGACATGAATACTTCGGATATGCATCCGttt                                                  >  1:4894266/1‑37 (MQ=255)

                                                  aaaaGACGACATGAATACTTCGGATATGCATCCGttt                                                  >  1:2981256/1‑37 (MQ=255)

                                                  aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGcc                 <  1:2971644/70‑1 (MQ=255)

                                                       acgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc                              <  1:4214709/52‑1 (MQ=255)

                                                        cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt                                     >  1:796807/1‑42 (MQ=25)

                                                        cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt                                     >  1:2625660/1‑42 (MQ=25)

                                                        cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt                                     >  1:1265174/1‑42 (MQ=25)

                                                         gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg          <  1:2757281/70‑1 (MQ=255)

                                                         gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg          <  1:673802/70‑1 (MQ=255)

                                                         gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg          <  1:5605469/70‑1 (MQ=255)

                                                          ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcgg                               <  1:3226024/46‑1 (MQ=255)

                                                          ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcgg                               <  1:3953829/46‑1 (MQ=255)

                                                          ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcgg                               <  1:4017036/46‑1 (MQ=255)

                                                          ataTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcgg                               <  1:4542792/46‑1 (MQ=255)

                                                          acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa                        <  1:295201/55‑1 (MQ=255)

                                                           cATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg                                      >  1:4451928/1‑40 (MQ=255)

                                                            aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcggcg                             >  1:1377363/1‑48 (MQ=255)

                                                            aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcggcg                             >  1:761349/1‑48 (MQ=255)

                                                            aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTgcagcg                             >  1:1760693/1‑48 (MQ=255)

                                                            aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCg                         <  1:52347/52‑1 (MQ=37)

                                                            aTGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa                        <  1:3625764/53‑1 (MQ=37)

                                                             tGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATc                      <  1:2116227/54‑1 (MQ=255)

                                                             tGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGa       <  1:2070046/69‑1 (MQ=255)

                                                              gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGaa      >  1:4454508/1‑69 (MQ=255)

                                                               aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg          >  1:1726496/1‑64 (MQ=255)

                                                                aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc  <  1:3035158/71‑1 (MQ=255)

AGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGC  >  minE/833874‑834008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A