Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,932,982 G→A 25.7% T487T (ACC→ACT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,932,9820GA25.7% 211.5 / 76.9 129T487T (ACC→ACT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (55/39);  new base A (20/13);  total (77/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

CATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTG  >  minE/1932912‑1933052
                                                                      |                                                                      
  tAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt                                                                      >  1:2800879/1‑71 (MQ=255)
    aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg                                                                     >  1:1143899/1‑70 (MQ=255)
    aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg                                                                     <  1:2485013/70‑1 (MQ=255)
           gCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg                                                                       >  1:4501457/1‑61 (MQ=255)
            cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGctt                                                             <  1:905567/70‑3 (MQ=255)
            cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt                                                             <  1:3173011/70‑1 (MQ=255)
            cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt                                                             <  1:287165/70‑1 (MQ=255)
            cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt                                                             <  1:1281469/70‑1 (MQ=255)
             ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa                                                                 >  1:3424891/1‑65 (MQ=255)
             ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa                                                           >  1:2810753/1‑71 (MQ=255)
             ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa                                                           >  1:80001/1‑71 (MQ=255)
               aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                          <  1:1140601/70‑1 (MQ=255)
               aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                          <  1:4162378/70‑1 (MQ=255)
                ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGTATGGTATGGCTGTTGGTGa                                                                    >  1:34734/1‑59 (MQ=255)
                ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                        >  1:152248/1‑71 (MQ=255)
                ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                        >  1:2969026/1‑71 (MQ=255)
                ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGGTg                                                                        >  1:723710/1‑55 (MQ=255)
                   tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATAc                                                     >  1:4696342/1‑71 (MQ=255)
                     ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg                                                    <  1:5317859/70‑1 (MQ=255)
                     ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg                                                    <  1:5661682/70‑1 (MQ=255)
                     ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg                                                    <  1:469845/70‑1 (MQ=255)
                        gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGaa                                                                   >  1:1195276/1‑52 (MQ=255)
                        gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                 >  1:4596429/1‑70 (MQ=255)
                          cGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGa                                               >  1:1911385/1‑70 (MQ=255)
                              tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                          <  1:4976200/55‑1 (MQ=255)
                               cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                         >  1:214042/1‑71 (MQ=255)
                               cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                         >  1:4480796/1‑71 (MQ=255)
                               cGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTTTGTAAACAGCATAATCATTCGGt                                                  >  1:4371568/1‑62 (MQ=255)
                                gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                         >  1:4613870/1‑70 (MQ=255)
                                gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                         >  1:1222664/1‑70 (MQ=255)
                                gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                         >  1:1549438/1‑70 (MQ=255)
                                gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                         >  1:1121669/1‑70 (MQ=255)
                                gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa                                         >  1:4542885/1‑70 (MQ=255)
                                gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa                                                                 >  1:3372839/1‑46 (MQ=255)
                                gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa                                                                 >  1:1871851/1‑46 (MQ=255)
                                gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa                                                                 >  1:195424/1‑46 (MQ=255)
                                gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt                                        >  1:2515203/1‑71 (MQ=255)
                                gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt                                        >  1:596568/1‑71 (MQ=255)
                                 ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg                                                                >  1:5675664/1‑46 (MQ=255)
                                 ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCa                                                              >  1:2797841/1‑48 (MQ=255)
                                 ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa                                                      >  1:615725/1‑56 (MQ=255)
                                 ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa                                                      >  1:1964495/1‑56 (MQ=255)
                                  cGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa                                               >  1:2007391/1‑62 (MQ=255)
                                   gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa                                         >  1:538936/1‑67 (MQ=255)
                                   gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa                                         >  1:166024/1‑67 (MQ=255)
                                   gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                     <  1:1912456/71‑1 (MQ=255)
                                    ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                           <  1:1215495/64‑1 (MQ=255)
                                    ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAccc                                    <  1:2474814/71‑1 (MQ=255)
                                      aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAcc                                     >  1:4509101/4‑68 (MQ=255)
                                      aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCa                                   <  1:4105807/67‑1 (MQ=255)
                                      aaCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                     >  1:605765/1‑68 (MQ=255)
                                        cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                                >  1:2554270/1‑71 (MQ=255)
                                         aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa                                                           >  1:3687344/1‑43 (MQ=255)
                                         aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa                                                           >  1:3349004/1‑43 (MQ=255)
                                         aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa                                                           >  1:4210291/1‑43 (MQ=255)
                                         aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                                 >  1:2029828/1‑53 (MQ=255)
                                         aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc                                    <  1:4848811/66‑1 (MQ=255)
                                          gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                              >  1:539040/1‑71 (MQ=255)
                                          gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                              >  1:2071754/1‑71 (MQ=255)
                                          gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                              >  1:4361469/1‑71 (MQ=255)
                                          gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                              >  1:1768795/1‑71 (MQ=255)
                                          gAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc                                >  1:591399/1‑69 (MQ=255)
                                           aaTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAccag                             >  1:3842704/1‑69 (MQ=255)
                                            aTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGa                                 >  1:4995649/1‑66 (MQ=255)
                                               aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                       <  1:5310255/57‑1 (MQ=255)
                                                cGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                       <  1:3724019/56‑1 (MQ=255)
                                                cGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                       <  1:5224403/56‑1 (MQ=255)
                                                 gTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCa                                          >  1:2743370/1‑52 (MQ=255)
                                                  tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                      <  1:5073843/71‑1 (MQ=255)
                                                  tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                           <  1:1028680/50‑1 (MQ=255)
                                                  tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                           <  1:4781095/50‑1 (MQ=255)
                                                  tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                           <  1:1262694/50‑1 (MQ=255)
                                                  tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                           <  1:405258/50‑1 (MQ=255)
                                                  tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg                           <  1:2874951/66‑2 (MQ=255)
                                                    gcgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc                                    <  1:1094365/55‑1 (MQ=255)
                                                      ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                             <  1:2438293/60‑1 (MQ=255)
                                                      ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                  <  1:834487/71‑1 (MQ=255)
                                                       gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                  >  1:1655341/1‑70 (MQ=255)
                                                       gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt                  >  1:561937/1‑70 (MQ=255)
                                                           gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc              >  1:1587734/1‑70 (MQ=255)
                                                           gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc              >  1:3022853/1‑70 (MQ=255)
                                                            tATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg                          <  1:3806969/57‑3 (MQ=255)
                                                            tATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg                          <  1:2880802/57‑3 (MQ=255)
                                                                gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt        >  1:4266918/1‑71 (MQ=255)
                                                                gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt        >  1:259517/1‑71 (MQ=255)
                                                                gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt         >  1:5237861/1‑70 (MQ=255)
                                                                 cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc       <  1:2287609/71‑1 (MQ=255)
                                                                 cTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg                          >  1:3006644/1‑50 (MQ=255)
                                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTTCTCTCGGCGGTAAGCCATGTTCCa     <  1:227321/70‑1 (MQ=255)
                                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc      <  1:5648073/69‑1 (MQ=255)
                                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc      <  1:2511018/69‑1 (MQ=255)
                                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc      <  1:498485/69‑1 (MQ=255)
                                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc      <  1:692037/69‑1 (MQ=255)
                                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa     <  1:2188498/70‑1 (MQ=255)
                                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg    >  1:106959/1‑71 (MQ=255)
                                                                    ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGAGGTa                 >  1:163794/1‑58 (MQ=255)
                                                                      ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                                >  1:639598/1‑41 (MQ=255)
                                                                      ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg  >  1:3662225/1‑71 (MQ=255)
                                                                      ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg  >  1:4782943/1‑71 (MQ=255)
                                                                      ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg  >  1:409699/1‑71 (MQ=255)
                                                                      |                                                                      
CATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTG  >  minE/1932912‑1933052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A