Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,081 C→G 34.1% A454A (GCG→GCC)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,0810CG34.1% 238.3 / 190.4 208A454A (GCG→GCC) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (70/67);  new base G (44/27);  total (114/94)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 215 total aligned reads displayed.

CAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCA  >  minE/1933011‑1933150
                                                                      |                                                                     
cAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                                                                       >  1:5193060/1‑71 (MQ=255)
  aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc                                                                     >  1:2034544/1‑71 (MQ=255)
  aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc                                                                     <  1:1390989/71‑1 (MQ=255)
    tcacAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt                                                                   >  1:227026/4‑71 (MQ=255)
     cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc                                                                  >  1:2157477/1‑71 (MQ=255)
      ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg                                                                 <  1:2704678/71‑1 (MQ=255)
      ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg                                                                 >  1:4437206/1‑71 (MQ=255)
      ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc                                                                  <  1:867354/70‑1 (MQ=255)
       cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt                                                                >  1:3588923/1‑71 (MQ=255)
        aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgt                                                                >  1:4269045/1‑70 (MQ=255)
         gACCTGCTGTCGGCGGTAAGCCATGTTGCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtcg                                                              >  1:2576131/1‑71 (MQ=255)
          aCCTTCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt                                                             <  1:5632619/71‑1 (MQ=255)
           ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc                                                            <  1:857411/71‑1 (MQ=255)
              gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc                                                                  >  1:623682/1‑62 (MQ=255)
               ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt                                                        >  1:1489893/1‑71 (MQ=255)
                 gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc                                                               >  1:4888566/1‑62 (MQ=255)
                 gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt                                                                >  1:5508692/1‑61 (MQ=255)
                    ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt                                                                >  1:5431395/1‑58 (MQ=255)
                    ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCCTCGTCGTCACCTGCGTc                                                   >  1:2235179/1‑71 (MQ=255)
                     gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt                                                  <  1:132381/71‑1 (MQ=255)
                        gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc                                                                  <  1:1561213/52‑1 (MQ=255)
                        gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc                                               <  1:4807766/71‑1 (MQ=255)
                        gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc                                               <  1:3262295/71‑1 (MQ=255)
                         tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt                                                             <  1:141426/56‑1 (MQ=255)
                         tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt                                                             <  1:2332597/56‑1 (MQ=255)
                         tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                              <  1:5427428/71‑1 (MQ=255)
                          aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt                                                             >  1:663315/1‑55 (MQ=255)
                          aaGCCAAGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc                                             >  1:2210741/1‑71 (MQ=255)
                           aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt                                                                >  1:3367552/1‑51 (MQ=255)
                           aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc                                                          >  1:5115191/1‑57 (MQ=255)
                           aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc                                                          >  1:3049866/1‑57 (MQ=255)
                           aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc                                                          >  1:2749332/1‑57 (MQ=255)
                             ccATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCt                                                  <  1:1116696/63‑1 (MQ=255)
                             ccATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtc                                                                  <  1:1729618/47‑1 (MQ=255)
                              cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc                                          >  1:2967475/1‑70 (MQ=255)
                               aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCaa                                        >  1:1084495/1‑71 (MQ=255)
                                tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt                                                  <  1:3339026/60‑1 (MQ=255)
                                tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                            <  1:3178810/66‑1 (MQ=255)
                                  ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt                                     >  1:3531122/1‑71 (MQ=255)
                                  ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag                                              >  1:1858649/1‑60 (MQ=255)
                                  ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag                                              >  1:3308526/1‑60 (MQ=255)
                                   tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                                                                       >  1:3768674/1‑36 (MQ=255)
                                   tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                                                                       >  1:3686878/1‑36 (MQ=255)
                                   tCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTg                                    >  1:2664724/1‑71 (MQ=255)
                                    ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa                                   >  1:369527/1‑71 (MQ=255)
                                    ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCttta                                               >  1:693925/1‑58 (MQ=255)
                                    ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc                                             >  1:2511602/1‑61 (MQ=255)
                                    ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                   >  1:3222538/1‑70 (MQ=255)
                                    ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                   >  1:4727515/1‑70 (MQ=255)
                                     cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCtt                                                                   >  1:546473/1‑38 (MQ=255)
                                     cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCAc                                                          >  1:5659632/1‑47 (MQ=255)
                                     cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                  <  1:2131891/71‑3 (MQ=255)
                                      aGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                              >  1:2415427/1‑58 (MQ=255)
                                       gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa                                                           <  1:5014813/44‑1 (MQ=255)
                                       gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa                                                           <  1:3920011/44‑1 (MQ=255)
                                        tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt                                                  <  1:4422532/52‑1 (MQ=255)
                                        tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                 >  1:1347867/1‑69 (MQ=255)
                                            gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                <  1:1990504/66‑1 (MQ=255)
                                            gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAgg                             >  1:2885049/1‑69 (MQ=255)
                                              caCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaac                         >  1:3999744/2‑69 (MQ=255)
                                               aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag                                              >  1:5537666/1‑47 (MQ=255)
                                               aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTcctg                              >  1:1652409/1‑63 (MQ=255)
                                               aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                        <  1:1152242/71‑1 (MQ=255)
                                               aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                                    <  1:1086110/59‑1 (MQ=255)
                                               aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                         <  1:4591973/70‑1 (MQ=255)
                                                 gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                            >  1:4627129/1‑48 (MQ=19)
                                                 gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                            >  1:5189370/1‑48 (MQ=19)
                                                 gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                      <  1:2052306/71‑1 (MQ=255)
                                                 gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                         <  1:2108966/68‑1 (MQ=255)
                                                   aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa                                         <  1:1507352/50‑1 (MQ=255)
                                                    aTCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca                                            >  1:2153076/1‑45 (MQ=16)
                                                    aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                   >  1:5333516/1‑71 (MQ=255)
                                                    aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                    <  1:2946673/70‑1 (MQ=255)
                                                      cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGt                                                    >  1:5391943/1‑36 (MQ=255)
                                                      cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc                                                   >  1:2245386/1‑37 (MQ=255)
                                                      cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                 >  1:5031790/1‑71 (MQ=255)
                                                       ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                            >  1:4352900/1‑43 (MQ=255)
                                                       ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                            >  1:3642964/1‑43 (MQ=255)
                                                       ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                            >  1:1201384/1‑43 (MQ=255)
                                                       ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg                                              >  1:4335824/1‑41 (MQ=255)
                                                       ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                >  1:2063233/1‑71 (MQ=255)
                                                       ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                >  1:2486038/1‑71 (MQ=255)
                                                         cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc                                          <  1:1238746/43‑1 (MQ=255)
                                                         cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg               <  1:3157432/70‑1 (MQ=255)
                                                         cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa              >  1:728693/1‑71 (MQ=255)
                                                           tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATc            <  1:2769368/68‑1 (MQ=255)
                                                            catAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt           >  1:3431730/3‑71 (MQ=255)
                                                             atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                       <  1:557120/57‑1 (MQ=255)
                                                             atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt          >  1:4084607/2‑71 (MQ=255)
                                                             atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt           >  1:3321430/2‑70 (MQ=255)
                                                              tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                      <  1:1038443/58‑1 (MQ=255)
                                                              tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                      <  1:3810730/58‑1 (MQ=255)
                                                               aGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct        >  1:3618334/1‑70 (MQ=255)
                                                                gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg       >  1:157900/1‑69 (MQ=255)
                                                                gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct        <  1:4171349/70‑2 (MQ=255)
                                                                gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg       >  1:4037584/1‑71 (MQ=255)
                                                                   cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                 <  1:5451451/58‑1 (MQ=255)
                                                                   cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                 <  1:2982565/58‑1 (MQ=255)
                                                                   cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                 <  1:4142501/58‑1 (MQ=255)
                                                                      cGCTTCGTCGTCACCTGCGTCTTTCGCTGCAACCTGATCCAGGAAGc                         <  1:1793986/47‑1 (MQ=255)
                                                                      |                                                                     
CAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCA  >  minE/1933011‑1933150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A