Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,108 G→A 43.4% A445A (GCC→GCT) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1080GA43.4% 58.6 / 151.9 132A445A (GCC→GCT) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (37/37);  new base A (25/32);  total (62/70)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 149 total aligned reads displayed.

AGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTT  >  minE/1933038‑1933178
                                                                      |                                                                      
   cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc                                                                      >  1:2967475/1‑70 (MQ=255)
    aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCaa                                                                    >  1:1084495/1‑71 (MQ=255)
     tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        <  1:3178810/66‑1 (MQ=255)
     tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        <  1:1839057/66‑1 (MQ=255)
     tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        <  1:3206314/66‑1 (MQ=255)
     tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        <  1:4510530/66‑1 (MQ=255)
        tCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTg                                                                >  1:2664724/1‑71 (MQ=255)
         ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa                                                               >  1:369527/1‑71 (MQ=255)
         ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                               >  1:4727515/1‑70 (MQ=255)
         ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                               >  1:1492064/1‑70 (MQ=255)
         ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                               >  1:3222538/1‑70 (MQ=255)
         ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                               >  1:1089950/1‑70 (MQ=255)
          cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                              <  1:2131891/71‑3 (MQ=255)
          cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                              <  1:2131888/71‑3 (MQ=255)
          cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                              <  1:691635/71‑3 (MQ=255)
            gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                                             >  1:476922/1‑70 (MQ=255)
            gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                                             >  1:2973139/1‑70 (MQ=255)
             tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                                             >  1:1347867/1‑69 (MQ=255)
                 gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                            <  1:208561/66‑1 (MQ=255)
                 gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                            <  1:1990504/66‑1 (MQ=255)
                 gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAgg                                                         >  1:2885049/1‑69 (MQ=255)
                    aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTcctg                                                          >  1:1652409/1‑63 (MQ=255)
                    aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                                    <  1:1152242/71‑1 (MQ=255)
                    aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                                    >  1:5455186/1‑71 (MQ=255)
                    aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                                                                <  1:1086110/59‑1 (MQ=255)
                    aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                                                                <  1:140950/59‑1 (MQ=255)
                    aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                                     <  1:5499635/70‑1 (MQ=255)
                    aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                                     <  1:4591973/70‑1 (MQ=255)
                     cGTAATCTTCAACGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAcc                                                                  <  1:835081/56‑1 (MQ=255)
                      gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                                    <  1:251110/69‑1 (MQ=255)
                      gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                                  >  1:2153822/1‑71 (MQ=255)
                       tAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGttc                                                 <  1:145909/71‑2 (MQ=255)
                         aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCacc                                                                   >  1:22391/1‑49 (MQ=255)
                         aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                                               >  1:5262763/1‑71 (MQ=255)
                         aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                                               >  1:5333516/1‑71 (MQ=255)
                         aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGCCAACCTGATCCAGGAAGCGTTgcg                                               >  1:3048664/1‑71 (MQ=255)
                          tCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                                    <  1:3560192/65‑1 (MQ=255)
                          tCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                <  1:3514704/69‑1 (MQ=255)
                           cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                            >  1:1760708/1‑56 (MQ=255)
                           cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGa                                                        >  1:401019/1‑60 (MQ=255)
                           cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                             >  1:5031790/1‑71 (MQ=255)
                            ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaa                                                      >  1:3260059/1‑60 (MQ=255)
                            ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGa                                                        <  1:5156722/59‑1 (MQ=255)
                            ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        >  1:4230971/1‑43 (MQ=255)
                            ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        >  1:1201384/1‑43 (MQ=255)
                            ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        >  1:3642964/1‑43 (MQ=255)
                            ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        >  1:4352900/1‑43 (MQ=255)
                            ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg                                                                        >  1:1303626/1‑43 (MQ=255)
                            ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                              >  1:5430545/1‑69 (MQ=255)
                            ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                            >  1:2063233/1‑71 (MQ=255)
                            ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                            >  1:2486038/1‑71 (MQ=255)
                              cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc                                                                      <  1:1238746/43‑1 (MQ=255)
                              cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGaa                                                       <  1:3334116/58‑1 (MQ=255)
                              cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                              <  1:3314824/67‑1 (MQ=255)
                              cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                            <  1:5634821/69‑1 (MQ=255)
                              cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa                                          >  1:728693/1‑71 (MQ=255)
                               aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa                                          >  1:462059/1‑70 (MQ=255)
                                tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATc                                        <  1:2769368/68‑1 (MQ=255)
                                 catAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                       >  1:3431730/3‑71 (MQ=255)
                                  atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                                   <  1:557120/57‑1 (MQ=255)
                                  atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                      >  1:504192/2‑71 (MQ=255)
                                  atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                       <  1:1578500/69‑1 (MQ=255)
                                   tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                                  <  1:3810730/58‑1 (MQ=255)
                                   tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                                  <  1:1038443/58‑1 (MQ=255)
                                   tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTc                                     <  1:5379538/71‑1 (MQ=255)
                                    aGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct                                    >  1:3618334/1‑70 (MQ=255)
                                     gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg                                   >  1:157900/1‑69 (MQ=255)
                                     gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg                                   >  1:5693059/1‑69 (MQ=255)
                                     gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                   >  1:4037584/1‑71 (MQ=255)
                                        cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt                                >  1:2141943/1‑71 (MQ=255)
                                        cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt                                                   >  1:3133129/1‑52 (MQ=255)
                                        cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                             <  1:5451451/58‑1 (MQ=255)
                                        cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                             <  1:4142501/58‑1 (MQ=255)
                                        cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                             <  1:2982565/58‑1 (MQ=255)
                                          tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                              <  1:4602582/69‑2 (MQ=255)
                                          tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa                              <  1:3810493/71‑1 (MQ=255)
                                          tCGCTTCGTCGTCACCTGCGTCTTTCGCGCCAACCt                                                                 >  1:4109142/1‑36 (MQ=255)
                                           ggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcgt                             >  1:3469952/2‑69 (MQ=255)
                                           ggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                              <  1:567082/69‑2 (MQ=255)
                                            gCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                      >  1:2250125/1‑61 (MQ=255)
                                             cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt                                         >  1:2837605/1‑57 (MQ=255)
                                               tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt                           <  1:102543/69‑2 (MQ=255)
                                               tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt                           <  1:1668496/69‑2 (MQ=255)
                                                cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                         <  1:185405/70‑4 (MQ=255)
                                                 gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                         >  1:2656606/1‑66 (MQ=255)
                                                 gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                         >  1:3920543/1‑66 (MQ=255)
                                                  tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc                      >  1:2887221/1‑71 (MQ=255)
                                                  tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc                      <  1:5644894/71‑1 (MQ=255)
                                                   cgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTc                                     <  1:2014241/55‑1 (MQ=255)
                                                    gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc                    >  1:5584112/1‑71 (MQ=255)
                                                     tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt                   <  1:5443102/71‑1 (MQ=255)
                                                            cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc             <  1:3043267/70‑1 (MQ=255)
                                                               cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa         >  1:2278565/1‑71 (MQ=255)
                                                               cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa         >  1:3019898/1‑71 (MQ=255)
                                                                 ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt           >  1:1723802/1‑67 (MQ=255)
                                                                  tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc       <  1:3059724/70‑1 (MQ=255)
                                                                    gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg                     >  1:3878992/1‑54 (MQ=255)
                                                                    gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg                     >  1:4927850/1‑54 (MQ=255)
                                                                      ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt  <  1:4699661/71‑1 (MQ=255)
                                                                      ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt  <  1:234870/71‑1 (MQ=255)
                                                                      |                                                                      
AGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTT  >  minE/1933038‑1933178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A