Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,110 C→T 41.3% A445T (GCC→ACC) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1100CT41.3% 83.3 / 150.1 138A445T (GCC→ACC) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (40/41);  new base T (25/32);  total (65/73)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

CATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCA  >  minE/1933041‑1933180
                                                                     |                                                                      
cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc                                                                        >  1:2967475/1‑70 (MQ=255)
 aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCaa                                                                      >  1:1084495/1‑71 (MQ=255)
 aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGAGTCTTTCGCGGCaa                                                                      >  1:226970/1‑71 (MQ=255)
    ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt                                                                   >  1:3531122/1‑71 (MQ=255)
     tCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTg                                                                  >  1:2664724/1‑71 (MQ=255)
      ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa                                                                 >  1:369527/1‑71 (MQ=255)
      ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                                 >  1:3222538/1‑70 (MQ=255)
      ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                                 >  1:1089950/1‑70 (MQ=255)
      ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                                 >  1:1492064/1‑70 (MQ=255)
      ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt                                                                 >  1:4727515/1‑70 (MQ=255)
       cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                                <  1:2131891/71‑3 (MQ=255)
       cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                                <  1:2131888/71‑3 (MQ=255)
       cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt                                                                <  1:691635/71‑3 (MQ=255)
          tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc                                                               >  1:1347867/1‑69 (MQ=255)
              gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                              <  1:208561/66‑1 (MQ=255)
              gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                              <  1:1990504/66‑1 (MQ=255)
              gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAgg                                                           >  1:2885049/1‑69 (MQ=255)
                caCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaac                                                       >  1:3999744/2‑69 (MQ=255)
                 aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTcctg                                                            >  1:1652409/1‑63 (MQ=255)
                 aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                                      <  1:2627342/71‑1 (MQ=255)
                 aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                                      <  1:1152242/71‑1 (MQ=255)
                 aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                                                                  <  1:140950/59‑1 (MQ=255)
                 aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg                                                                  <  1:1086110/59‑1 (MQ=255)
                 aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                                       <  1:5499635/70‑1 (MQ=255)
                 aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                                       <  1:4591973/70‑1 (MQ=255)
                  cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                                     <  1:344419/71‑1 (MQ=255)
                  cGTAATCTTCAACGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAcc                                                                    <  1:835081/56‑1 (MQ=255)
                   gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                                      <  1:251110/69‑1 (MQ=255)
                   gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                                    <  1:2052306/71‑1 (MQ=255)
                   gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                                    >  1:2153822/1‑71 (MQ=255)
                   gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                                    <  1:1957255/71‑1 (MQ=255)
                   gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                                       <  1:5420870/68‑1 (MQ=255)
                    tAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGttc                                                   <  1:145909/71‑2 (MQ=255)
                     aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt                                                     <  1:2769100/68‑1 (MQ=255)
                      aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCacc                                                                     >  1:22391/1‑49 (MQ=255)
                      aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                                                 >  1:5262763/1‑71 (MQ=255)
                      aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                                                 >  1:5333516/1‑71 (MQ=255)
                      aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                  <  1:2946673/70‑1 (MQ=255)
                      aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                  <  1:1217528/70‑1 (MQ=255)
                      aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGCCAACCTGATCCAGGAAGCGTTgcg                                                 >  1:3048664/1‑71 (MQ=255)
                       tCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg                                                      <  1:3560192/65‑1 (MQ=255)
                       tCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                  <  1:3514704/69‑1 (MQ=255)
                        cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc                                                              >  1:1760708/1‑56 (MQ=255)
                        cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                               >  1:5031790/1‑71 (MQ=255)
                         ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaa                                                        >  1:3260059/1‑60 (MQ=255)
                         ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGa                                                          <  1:5156722/59‑1 (MQ=255)
                         ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                               >  1:1685781/1‑70 (MQ=255)
                         ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                              >  1:2063233/1‑71 (MQ=255)
                           cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGaa                                                         <  1:3334116/58‑1 (MQ=255)
                           cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                              <  1:5634821/69‑1 (MQ=255)
                           cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa                                            >  1:728693/1‑71 (MQ=255)
                            aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa                                            >  1:462059/1‑70 (MQ=255)
                             tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATc                                          <  1:2769368/68‑1 (MQ=255)
                              catAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                         >  1:3431730/3‑71 (MQ=255)
                               atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                                     <  1:4595056/57‑1 (MQ=255)
                               atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                        >  1:4084607/2‑71 (MQ=255)
                               atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                         >  1:3321430/2‑70 (MQ=255)
                               atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                         <  1:1578500/69‑1 (MQ=255)
                                tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                                    <  1:3810730/58‑1 (MQ=255)
                                tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt                                                    <  1:1038443/58‑1 (MQ=255)
                                tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTc                                       <  1:5379538/71‑1 (MQ=255)
                                  gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg                                     >  1:157900/1‑69 (MQ=255)
                                  gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg                                     >  1:5693059/1‑69 (MQ=255)
                                  gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct                                      <  1:4171349/70‑2 (MQ=255)
                                  gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                     >  1:4037584/1‑71 (MQ=255)
                                   aaCATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcgc                                    <  1:2846754/71‑1 (MQ=255)
                                     cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt                                  <  1:4430440/71‑1 (MQ=255)
                                     cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt                                  >  1:2141943/1‑71 (MQ=255)
                                     cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTACTGGAAACGTTCAGCCTGATCTTCTGCGt                                  >  1:2382617/1‑71 (MQ=255)
                                     cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt                                                     >  1:3133129/1‑52 (MQ=255)
                                     cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                               <  1:4142501/58‑1 (MQ=255)
                                     cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                               <  1:5451451/58‑1 (MQ=255)
                                     cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                               <  1:2982565/58‑1 (MQ=255)
                                     cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                               <  1:3622461/58‑1 (MQ=255)
                                       tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa                                <  1:3810493/71‑1 (MQ=255)
                                        ggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                                <  1:567082/69‑2 (MQ=255)
                                        cGCTTCGTCGTCACCTGCGTCTTTCGCTGCAACCTGATCCAGGAAGc                                                       <  1:1793986/47‑1 (MQ=255)
                                         gCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                        >  1:2250125/1‑61 (MQ=255)
                                          cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGtt                                                    <  1:51440/48‑1 (MQ=255)
                                          cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc                             <  1:2975721/71‑1 (MQ=255)
                                            tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt                             <  1:102543/69‑2 (MQ=255)
                                            tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt                             <  1:1668496/69‑2 (MQ=255)
                                             cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                           <  1:5573518/70‑4 (MQ=255)
                                              gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                           >  1:3920543/1‑66 (MQ=255)
                                               tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc                        <  1:5644894/71‑1 (MQ=255)
                                               tcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTc                                 <  1:4628986/62‑1 (MQ=255)
                                                 gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAg                                                        <  1:4729331/37‑1 (MQ=255)
                                                 gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc                      >  1:5584112/1‑71 (MQ=255)
                                                  tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt                     <  1:5443102/71‑1 (MQ=255)
                                                         cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc               <  1:3043267/70‑1 (MQ=255)
                                                           tCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt             >  1:5461608/1‑70 (MQ=255)
                                                            cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa           >  1:3019898/1‑71 (MQ=255)
                                                              ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc                >  1:4955044/1‑64 (MQ=255)
                                                               tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc         <  1:3059724/70‑1 (MQ=255)
                                                                cgcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATc                                          >  1:3234368/1‑36 (MQ=255)
                                                                 gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg                       >  1:3878992/1‑54 (MQ=255)
                                                                 gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg                       >  1:4927850/1‑54 (MQ=255)
                                                                   ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt    <  1:234870/71‑1 (MQ=255)
                                                                    gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTc   <  1:1266388/71‑1 (MQ=255)
                                                                     cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa  >  1:5270196/1‑71 (MQ=255)
                                                                     |                                                                      
CATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCA  >  minE/1933041‑1933180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A