Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,131 G→C 37.5% Q438E (CAA→GAA)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1310GC37.5% 128.2 / 154.6 152Q438E (CAA→GAA) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (44/51);  new base C (24/33);  total (68/84)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

Only 100 of 167 total aligned reads displayed.

ATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTC  >  minE/1933063‑1933200
                                                                    |                                                                     
aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                                                                     >  1:5333516/1‑71 (MQ=255)
aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                                      <  1:2946673/70‑1 (MQ=255)
aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGCCAACCTGATCCAGGAAGCGTTgcg                                                                     >  1:3048664/1‑71 (MQ=255)
 tCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                                      <  1:3514704/69‑1 (MQ=255)
   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                                                   >  1:1685781/1‑70 (MQ=255)
   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                                                  >  1:2063233/1‑71 (MQ=255)
   ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                                                  >  1:2486038/1‑71 (MQ=255)
     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                                                    <  1:3314824/67‑1 (MQ=255)
     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                                                  <  1:5634821/69‑1 (MQ=255)
        catAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                                             >  1:3431730/3‑71 (MQ=255)
         atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                                            >  1:504192/2‑71 (MQ=255)
         atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt                                                            >  1:4084607/2‑71 (MQ=255)
         atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt                                                             <  1:1578500/69‑1 (MQ=255)
            gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg                                                         >  1:5693059/1‑69 (MQ=255)
            gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                                         >  1:4037584/1‑71 (MQ=255)
               cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt                                                      >  1:2141943/1‑71 (MQ=255)
               cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                                                   <  1:4142501/58‑1 (MQ=255)
               cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                                                   <  1:3622461/58‑1 (MQ=255)
               cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                                                   <  1:5451451/58‑1 (MQ=255)
               cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                                                   <  1:2982565/58‑1 (MQ=255)
                 tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                                                    <  1:5685779/69‑2 (MQ=255)
                 tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                                                    <  1:4602582/69‑2 (MQ=255)
                 tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa                                                    <  1:3810493/71‑1 (MQ=255)
                    cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt                                                               >  1:2837605/1‑57 (MQ=255)
                      tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt                                                 <  1:102543/69‑2 (MQ=255)
                       cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                                               <  1:5573518/70‑4 (MQ=255)
                       cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttta                                                <  1:1583054/69‑3 (MQ=255)
                        gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                                               >  1:2656606/1‑66 (MQ=255)
                         tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc                                            <  1:5644894/71‑1 (MQ=255)
                         tcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTc                                                     <  1:4628986/62‑1 (MQ=255)
                            tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt                                         <  1:1432638/71‑1 (MQ=255)
                            tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt                                         <  1:5443102/71‑1 (MQ=255)
                                   cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCg                                  >  1:3763480/1‑71 (MQ=255)
                                     tCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                                 >  1:5461608/1‑70 (MQ=255)
                                       tttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                              >  1:1110760/1‑71 (MQ=255)
                                        ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc                                    >  1:4955044/1‑64 (MQ=255)
                                         tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                             <  1:3059724/70‑1 (MQ=255)
                                         tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                              <  1:3032305/69‑1 (MQ=255)
                                          cgcgGCAACCTGATCCAGGACGCGTTGCGCCTGATc                                                              >  1:5638580/1‑36 (MQ=255)
                                           gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg                                           >  1:3878992/1‑54 (MQ=255)
                                             ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                        <  1:4699661/71‑1 (MQ=255)
                                             ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                        <  1:234870/71‑1 (MQ=255)
                                              ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCAtt                                <  1:3140496/60‑1 (MQ=255)
                                              ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCAtt                                <  1:5163388/60‑1 (MQ=255)
                                              ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGAttt                        >  1:4394814/3‑70 (MQ=255)
                                              ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc                       <  1:2611400/69‑1 (MQ=255)
                                              ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc                       <  1:1364007/69‑1 (MQ=255)
                                              gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                        <  1:5256005/70‑1 (MQ=255)
                                               taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa                                      <  1:2442884/54‑1 (MQ=255)
                                               taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa                                      <  1:2479176/54‑1 (MQ=255)
                                               cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa                      >  1:5270196/1‑71 (MQ=255)
                                                aaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCAc                     >  1:701376/1‑71 (MQ=255)
                                                aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                              <  1:1558429/62‑1 (MQ=255)
                                                aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                              <  1:2697084/62‑1 (MQ=255)
                                                aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                     <  1:333592/71‑1 (MQ=255)
                                                aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                     <  1:5395818/71‑1 (MQ=255)
                                                 aCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCa                      >  1:3909972/1‑69 (MQ=255)
                                                 aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc                                                     <  1:1230944/38‑1 (MQ=255)
                                                 aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc                                                     <  1:3042499/38‑1 (MQ=255)
                                                 aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                                                  >  1:3491150/1‑41 (MQ=255)
                                                  ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc                                                     <  1:1654214/37‑1 (MQ=255)
                                                  ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                                 >  1:2076823/1‑57 (MQ=255)
                                                  ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                             <  1:227153/61‑1 (MQ=255)
                                                   cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc                                                 >  1:4158752/1‑40 (MQ=255)
                                                    tGATCCAGGAAGCGTTGCGCTTGATCTTCCGCGTCATTc                                                 <  1:1818849/39‑1 (MQ=255)
                                                    tGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac                  >  1:1417998/1‑70 (MQ=255)
                                                     gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca                >  1:3179684/3‑71 (MQ=255)
                                                     gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca                >  1:5457012/3‑71 (MQ=255)
                                                     gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca                >  1:2647253/3‑71 (MQ=255)
                                                     gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt                                             <  1:5614661/42‑1 (MQ=255)
                                                     gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt                                             <  1:1987914/42‑1 (MQ=255)
                                                     gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca                >  1:3731627/1‑71 (MQ=255)
                                                      ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac               <  1:602000/70‑1 (MQ=255)
                                                      ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac               <  1:394704/70‑1 (MQ=255)
                                                      aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                             <  1:178907/57‑1 (MQ=255)
                                                      aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac               <  1:1624623/71‑1 (MQ=255)
                                                       tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCg                                  >  1:3529392/1‑51 (MQ=255)
                                                       tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac               >  1:4423919/1‑70 (MQ=255)
                                                        ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt                          >  1:2960401/1‑58 (MQ=255)
                                                         ctggAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccaccg             <  1:2123520/68‑2 (MQ=255)
                                                          tggAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGAt                          <  1:2787113/55‑1 (MQ=255)
                                                          aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt           <  1:43334/71‑1 (MQ=255)
                                                            gAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc                                    <  1:4036985/44‑1 (MQ=255)
                                                            gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTa                               >  1:328915/1‑49 (MQ=255)
                                                            gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGAttt                        >  1:4172145/1‑56 (MQ=255)
                                                            gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATga         <  1:2335700/71‑1 (MQ=255)
                                                            gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg          <  1:4985329/70‑1 (MQ=255)
                                                            gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg          <  1:2011664/70‑1 (MQ=255)
                                                            gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg          <  1:2241059/70‑1 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc                                   >  1:2189832/1‑44 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                        >  1:4831013/1‑55 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                        >  1:2858329/1‑55 (MQ=255)
                                                              aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag       <  1:1850030/71‑1 (MQ=255)
                                                              aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag       <  1:391826/71‑1 (MQ=255)
                                                               gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                        <  1:33644/53‑1 (MQ=255)
                                                               gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag       >  1:4574801/1‑70 (MQ=255)
                                                                  ttGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACt   <  1:5328050/71‑1 (MQ=255)
                                                                    gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                     >  1:4741048/1‑51 (MQ=255)
                                                                    gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                     >  1:3602863/1‑51 (MQ=255)
                                                                    gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCAGTTACCGATTTCACGa                   >  1:3826639/1‑53 (MQ=255)
                                                                    |                                                                     
ATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTC  >  minE/1933063‑1933200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A