Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,144 C→T 37.8% A433A (GCG→GCA)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1440CT37.8% 137.2 / 161.4 164A433A (GCG→GCA) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (52/50);  new base T (28/34);  total (80/84)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 172 total aligned reads displayed.

GAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTG  >  minE/1933075‑1933213
                                                                     |                                                                     
gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                                                      >  1:4037584/1‑71 (MQ=255)
 aaCATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcgc                                                                     <  1:2846754/71‑1 (MQ=255)
   cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt                                                                   <  1:4430440/71‑1 (MQ=255)
   cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTACTGGAAACGTTCAGCCTGATCTTCTGCGt                                                                   >  1:2382617/1‑71 (MQ=255)
     tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                                                                 <  1:304919/69‑2 (MQ=255)
     tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                                                                 <  1:4602582/69‑2 (MQ=255)
     tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa                                                                 <  1:3810493/71‑1 (MQ=255)
      ggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg                                                                 <  1:567082/69‑2 (MQ=255)
       gCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                                                               >  1:5288223/1‑71 (MQ=255)
        cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc                                                              <  1:2975721/71‑1 (MQ=255)
          tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt                                                              <  1:102543/69‑2 (MQ=255)
           cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                                                            <  1:185405/70‑4 (MQ=255)
            gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa                                                            >  1:2656606/1‑66 (MQ=255)
             tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc                                                         >  1:2887221/1‑71 (MQ=255)
               gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc                                                       >  1:5584112/1‑71 (MQ=255)
                    cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                                  >  1:2133253/1‑71 (MQ=255)
                       cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc                                                <  1:3043267/70‑1 (MQ=255)
                       cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCg                                               >  1:3763480/1‑71 (MQ=255)
                         tCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                                              >  1:5461608/1‑70 (MQ=255)
                          cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa                                            >  1:3019898/1‑71 (MQ=255)
                          cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa                                            >  1:2278565/1‑71 (MQ=255)
                            ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc                                                 >  1:4955044/1‑64 (MQ=255)
                            ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                                              >  1:1723802/1‑67 (MQ=255)
                               gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg                                                        >  1:3878992/1‑54 (MQ=255)
                                 ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                     <  1:4699661/71‑1 (MQ=255)
                                 ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                     <  1:234870/71‑1 (MQ=255)
                                  ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc                                    <  1:1364007/69‑1 (MQ=255)
                                  gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                     <  1:5256005/70‑1 (MQ=255)
                                  gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTc                                    <  1:1266388/71‑1 (MQ=255)
                                   taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa                                                   <  1:1425217/54‑1 (MQ=255)
                                   cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa                                   >  1:5270196/1‑71 (MQ=255)
                                    aaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCAc                                  >  1:701376/1‑71 (MQ=255)
                                    aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAg                                                            >  1:182549/1‑45 (MQ=255)
                                    aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                                           <  1:2697084/62‑1 (MQ=255)
                                    aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                                           <  1:1558429/62‑1 (MQ=255)
                                    aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                  <  1:333592/71‑1 (MQ=255)
                                    aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                  <  1:5395818/71‑1 (MQ=255)
                                     aCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCa                                   >  1:3909972/1‑69 (MQ=255)
                                     aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc                                                                  <  1:3042499/38‑1 (MQ=255)
                                     aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                                                               >  1:3491150/1‑41 (MQ=255)
                                      ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc                                                                  <  1:1654214/37‑1 (MQ=255)
                                      ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                                              >  1:2076823/1‑57 (MQ=255)
                                       cTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac                               <  1:1620121/71‑1 (MQ=255)
                                       cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc                                                              >  1:4158752/1‑40 (MQ=255)
                                       cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac                               <  1:5101936/71‑1 (MQ=255)
                                         gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca                             >  1:3179684/3‑71 (MQ=255)
                                         gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca                             >  1:571443/3‑71 (MQ=255)
                                         gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca                             >  1:2647253/3‑71 (MQ=255)
                                         gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt                                                          <  1:2515325/42‑1 (MQ=255)
                                         gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt                                                          <  1:1987914/42‑1 (MQ=255)
                                         gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt                                                          <  1:5614661/42‑1 (MQ=255)
                                         gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt                                                          <  1:5621368/42‑1 (MQ=255)
                                         gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                                  <  1:545302/50‑1 (MQ=255)
                                         gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca                             >  1:3731627/1‑71 (MQ=255)
                                          ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac                            <  1:602000/70‑1 (MQ=255)
                                          ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac                            <  1:394704/70‑1 (MQ=255)
                                          ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacc                              <  1:3514483/68‑1 (MQ=255)
                                           tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac                            >  1:2465566/1‑70 (MQ=255)
                                           tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac                            >  1:4423919/1‑70 (MQ=255)
                                            ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt                                       >  1:4631645/1‑58 (MQ=255)
                                            ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt                                       >  1:2960401/1‑58 (MQ=255)
                                              aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt                        <  1:4794449/71‑1 (MQ=255)
                                               ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt                        >  1:406378/1‑70 (MQ=255)
                                                gAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccacc                           <  1:4235684/66‑1 (MQ=255)
                                                gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTa                                            >  1:328915/1‑49 (MQ=255)
                                                gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg                       <  1:2241059/70‑1 (MQ=255)
                                                 aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                     >  1:4831013/1‑55 (MQ=255)
                                                  aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGa                                >  1:2620785/1‑59 (MQ=255)
                                                  aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag                    <  1:391826/71‑1 (MQ=255)
                                                  aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag                    <  1:5458297/71‑1 (MQ=255)
                                                   gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                     <  1:33644/53‑1 (MQ=255)
                                                        gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                  >  1:2181999/1‑51 (MQ=255)
                                                        gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                  >  1:3602863/1‑51 (MQ=255)
                                                        gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCaa                         >  1:1466576/1‑60 (MQ=255)
                                                        gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc               >  1:5641659/1‑70 (MQ=255)
                                                        gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCAGTTACCGATTTCACGa                                >  1:3826639/1‑53 (MQ=255)
                                                        cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                           >  1:1881319/3‑58 (MQ=255)
                                                        cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                           >  1:1140282/3‑58 (MQ=255)
                                                        cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATga                      >  1:4404693/3‑63 (MQ=255)
                                                        cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc               >  1:1032166/3‑70 (MQ=255)
                                                        cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc               >  1:4696600/3‑70 (MQ=255)
                                                         agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa             <  1:4362054/70‑1 (MQ=255)
                                                          gcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCa              >  1:1285278/1‑69 (MQ=255)
                                                           ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga                      <  1:4215128/60‑1 (MQ=255)
                                                           ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga                      <  1:2214339/60‑1 (MQ=255)
                                                            cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc          >  1:1817262/1‑71 (MQ=255)
                                                             tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                           <  1:353530/53‑1 (MQ=255)
                                                             tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                                          <  1:2022305/38‑1 (MQ=255)
                                                             tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                     >  1:5161285/1‑43 (MQ=255)
                                                             tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg         >  1:3995602/1‑71 (MQ=255)
                                                               aTCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccaccga                         >  1:5696095/1‑51 (MQ=255)
                                                                 cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac                               >  1:21220/1‑45 (MQ=255)
                                                                 cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTc      >  1:162298/1‑70 (MQ=255)
                                                                 cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa                                   >  1:2730274/1‑41 (MQ=255)
                                                                 cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa                                   >  1:3134762/1‑41 (MQ=255)
                                                                    ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc      <  1:4732972/67‑1 (MQ=255)
                                                                    ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt   <  1:148358/70‑1 (MQ=255)
                                                                    ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt     <  1:3784642/68‑1 (MQ=255)
                                                                    ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt     <  1:181014/68‑1 (MQ=255)
                                                                     cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg  <  1:5053157/70‑1 (MQ=255)
                                                                     |                                                                     
GAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTG  >  minE/1933075‑1933213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A