Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,153 C→T 31.1% L430L (CTG→CTA) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1530CT31.1% 191.5 / 113.4 148L430L (CTG→CTA) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (49/53);  new base T (23/23);  total (72/76)
Fisher's exact test for biased strand distribution p-value = 8.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 152 total aligned reads displayed.

CTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGG  >  minE/1933083‑1933222
                                                                      |                                                                     
cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc                                                                       <  1:2975721/71‑1 (MQ=255)
     tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc                                                                  <  1:5644894/71‑1 (MQ=255)
     tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc                                                                  >  1:2887221/1‑71 (MQ=255)
       gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc                                                                >  1:5584112/1‑71 (MQ=255)
        tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt                                                               <  1:1432638/71‑1 (MQ=255)
        tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt                                                               <  1:5443102/71‑1 (MQ=255)
            cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                                           >  1:2133253/1‑71 (MQ=255)
                  cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa                                                     >  1:2278565/1‑71 (MQ=255)
                  cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa                                                     >  1:3019898/1‑71 (MQ=255)
                   tttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                                                    >  1:1110760/1‑71 (MQ=255)
                    ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc                                                          >  1:4955044/1‑64 (MQ=255)
                    ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                                                       >  1:1723802/1‑67 (MQ=255)
                     tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                                                   <  1:3059724/70‑1 (MQ=255)
                     tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                                                    <  1:3032305/69‑1 (MQ=255)
                       gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg                                                                 >  1:4927850/1‑54 (MQ=255)
                         ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                              <  1:4699661/71‑1 (MQ=255)
                         ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                              <  1:234870/71‑1 (MQ=255)
                          ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCAtt                                                      <  1:3140496/60‑1 (MQ=255)
                          ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCAtt                                                      <  1:5163388/60‑1 (MQ=255)
                          ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGAttt                                              >  1:4394814/3‑70 (MQ=255)
                          ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc                                             <  1:1364007/69‑1 (MQ=255)
                          ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc                                             <  1:2611400/69‑1 (MQ=255)
                          gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTc                                             <  1:1266388/71‑1 (MQ=255)
                           taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa                                                            <  1:1425217/54‑1 (MQ=255)
                           taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa                                                            <  1:2479176/54‑1 (MQ=255)
                           cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa                                            >  1:5270196/1‑71 (MQ=255)
                            aaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCAc                                           >  1:701376/1‑71 (MQ=255)
                            aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                           <  1:5395818/71‑1 (MQ=255)
                            aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                           <  1:3734483/71‑1 (MQ=255)
                             aCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCa                                            >  1:3909972/1‑69 (MQ=255)
                              ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                                                       >  1:2076823/1‑57 (MQ=255)
                              ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                                                   <  1:227153/61‑1 (MQ=255)
                               cTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac                                        <  1:1620121/71‑1 (MQ=255)
                               cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc                                                                       >  1:2684931/1‑40 (MQ=255)
                               cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc                                                                       >  1:4158752/1‑40 (MQ=255)
                                tGATCCAGGAAGCGTTGCGCTTGATCTTCCGCGTCATTc                                                                       <  1:1818849/39‑1 (MQ=255)
                                 gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca                                      >  1:5457012/3‑71 (MQ=255)
                                 gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca                                      >  1:3179684/3‑71 (MQ=255)
                                 gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt                                                                   <  1:5621368/42‑1 (MQ=255)
                                 gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca                                      >  1:3731627/1‑71 (MQ=255)
                                  ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac                                     <  1:602000/70‑1 (MQ=255)
                                  ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac                                     <  1:394704/70‑1 (MQ=255)
                                  ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacc                                       <  1:3514483/68‑1 (MQ=255)
                                  aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                                                   <  1:178907/57‑1 (MQ=255)
                                   tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac                                     >  1:4423919/1‑70 (MQ=255)
                                   tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac                                     >  1:2465566/1‑70 (MQ=255)
                                    ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt                                                >  1:2960401/1‑58 (MQ=255)
                                      aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt                                 <  1:4794449/71‑1 (MQ=255)
                                       ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt                                 >  1:406378/1‑70 (MQ=255)
                                        gAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccacc                                    <  1:4235684/66‑1 (MQ=255)
                                        gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGAttt                                              >  1:4172145/1‑56 (MQ=255)
                                        gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg                                <  1:2011664/70‑1 (MQ=255)
                                         aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc                                                         >  1:2189832/1‑44 (MQ=255)
                                         aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                              >  1:2858329/1‑55 (MQ=255)
                                          aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGa                                         >  1:2620785/1‑59 (MQ=255)
                                          aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag                             <  1:5458297/71‑1 (MQ=255)
                                          aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag                             <  1:1850030/71‑1 (MQ=255)
                                           gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                              <  1:33644/53‑1 (MQ=255)
                                           gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                              <  1:3897859/53‑1 (MQ=255)
                                           gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag                             >  1:4574801/1‑70 (MQ=255)
                                             gTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa                                                     >  1:3597298/1‑44 (MQ=255)
                                                gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                           >  1:3773649/1‑51 (MQ=255)
                                                gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                           >  1:4741048/1‑51 (MQ=255)
                                                gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCAGTTACCGATTTCACGa                                         >  1:3826639/1‑53 (MQ=255)
                                                cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                                    >  1:1881319/3‑58 (MQ=255)
                                                cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                                    >  1:1547396/3‑58 (MQ=255)
                                                cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc                        >  1:1032166/3‑70 (MQ=255)
                                                 agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa                      <  1:4686757/70‑1 (MQ=255)
                                                 agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa                      <  1:3293834/70‑1 (MQ=255)
                                                 agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa                      <  1:4362054/70‑1 (MQ=255)
                                                 agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCa                       <  1:3045636/69‑1 (MQ=255)
                                                  gcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCa                       >  1:1285278/1‑69 (MQ=255)
                                                   ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga                               <  1:2214339/60‑1 (MQ=255)
                                                    cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc                   >  1:1817262/1‑71 (MQ=255)
                                                     tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                                    <  1:5623186/53‑1 (MQ=255)
                                                     tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                                    <  1:353530/53‑1 (MQ=255)
                                                     tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                                              >  1:5161285/1‑43 (MQ=255)
                                                     tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt                                                <  1:1982/41‑1 (MQ=255)
                                                     tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg                  >  1:3995602/1‑71 (MQ=255)
                                                      gATCTTCTGCGTCGTTTAATTCGCTAAAACCATTAACGATTTCACGACCACCGATGAAGAATTCAAAACgg                 >  1:579612/1‑71 (MQ=255)
                                                      gATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc                                           >  1:3852919/1‑45 (MQ=255)
                                                       aTCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccaccga                                  >  1:4704987/1‑51 (MQ=255)
                                                       aTCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccaccga                                  >  1:5696095/1‑51 (MQ=255)
                                                       aTCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt                >  1:5260041/1‑71 (MQ=255)
                                                         cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac                                        >  1:21220/1‑45 (MQ=255)
                                                         cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac                                        >  1:5594233/1‑45 (MQ=255)
                                                         cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTc               >  1:162298/1‑70 (MQ=255)
                                                         cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa                                            >  1:2730274/1‑41 (MQ=255)
                                                          ttCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTcgg             >  1:5093520/1‑69 (MQ=255)
                                                          ttCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCaa                                  >  1:2649409/1‑50 (MQ=255)
                                                            ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt            <  1:148358/70‑1 (MQ=255)
                                                            ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt              <  1:3784642/68‑1 (MQ=255)
                                                             cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg           <  1:5053157/70‑1 (MQ=255)
                                                                 tCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt       <  1:2735161/70‑1 (MQ=255)
                                                                  cATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc               <  1:1971917/61‑1 (MQ=255)
                                                                   aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt                                 <  1:2417751/42‑1 (MQ=255)
                                                                   aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTcc     <  1:1360530/70‑1 (MQ=255)
                                                                    ttCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCaa                                  <  1:4072196/40‑1 (MQ=255)
                                                                     tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg  >  1:2018706/1‑71 (MQ=255)
                                                                     tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg  >  1:4314297/1‑71 (MQ=255)
                                                                      |                                                                     
CTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGG  >  minE/1933083‑1933222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A