Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,204 G→A 49.5% R413R (CGC→CGT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,2040GA49.5% 10.3 / 147.3 109R413R (CGC→CGT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (30/25);  new base A (22/32);  total (52/57)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAA  >  minE/1933135‑1933274
                                                                     |                                                                      
cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc                                                                       >  1:1817262/1‑71 (MQ=255)
 tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg                                                                      >  1:3995602/1‑71 (MQ=255)
  gATCTTCTGCGTCGTTTAATTCGCTAAAACCATTAACGATTTCACGACCACCGATGAAGAATTCAAAACgg                                                                     >  1:579612/1‑71 (MQ=255)
   aTCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt                                                                    >  1:5260041/1‑71 (MQ=255)
     cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTc                                                                   >  1:162298/1‑70 (MQ=255)
     cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt                                                                  >  1:83250/1‑71 (MQ=255)
      ttCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTcgg                                                                 >  1:5093520/1‑69 (MQ=255)
        ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt                                                                    <  1:3795329/66‑1 (MQ=255)
        ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc                                                                   <  1:4732972/67‑1 (MQ=255)
        ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt                                                                <  1:148358/70‑1 (MQ=255)
        ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt                                                                  <  1:181014/68‑1 (MQ=255)
        ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt                                                                  <  1:3784642/68‑1 (MQ=255)
         cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg                                                               <  1:5053157/70‑1 (MQ=255)
           cgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAtt                                                            <  1:1958619/71‑1 (MQ=255)
             tCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt                                                           <  1:2735161/70‑1 (MQ=255)
              cATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc                                                                   <  1:1971917/61‑1 (MQ=255)
                 tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg                                                      >  1:4314297/1‑71 (MQ=255)
                 tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg                                                      >  1:2018706/1‑71 (MQ=255)
                   aGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGt                                                     >  1:3826387/1‑70 (MQ=255)
                    attCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt                                                    <  1:4205209/68‑1 (MQ=255)
                     ttCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTc                                                          <  1:3011458/62‑1 (MQ=255)
                     ttCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa                                                  >  1:1234982/2‑71 (MQ=255)
                      tCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAc                                                 >  1:3613736/1‑71 (MQ=255)
                        gCTAAAACCATTACCGATTTTACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcat                                               <  1:4925232/71‑3 (MQ=255)
                        gCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcat                                               <  1:321381/71‑3 (MQ=255)
                        gCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAca                                                <  1:5627787/70‑2 (MQ=255)
                           aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat                                            >  1:2156035/1‑69 (MQ=255)
                           aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat                                            >  1:2585388/1‑69 (MQ=255)
                             aGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc                                                                       <  1:5349877/42‑1 (MQ=255)
                              gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                                         >  1:3650974/1‑71 (MQ=255)
                              gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                                         >  1:3459425/1‑71 (MQ=255)
                              gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                                         >  1:1998655/1‑71 (MQ=255)
                              gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                                         >  1:2357625/1‑71 (MQ=255)
                                cattACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacgg                                       <  1:217585/69‑2 (MQ=255)
                                 gTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacgac                                      >  1:169675/1‑71 (MQ=255)
                                  ttAGCGATTTCACGACCACCGATGAAGAATTCAAAACg                                                                      <  1:5633858/38‑1 (MQ=25)
                                  ttACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat                                            <  1:4769723/64‑3 (MQ=255)
                                  ttACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt                                                           >  1:1390382/1‑49 (MQ=255)
                                   tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa                                                              >  1:5626423/1‑45 (MQ=255)
                                   tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa                                                              >  1:4416406/1‑45 (MQ=255)
                                   tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa                                                              >  1:3099723/1‑45 (MQ=255)
                                   gaCCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa                                                              >  1:5177432/2‑45 (MQ=255)
                                    aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAt                                                             >  1:3840602/1‑45 (MQ=255)
                                    aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg                                   >  1:1279968/1‑71 (MQ=255)
                                        aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat                                            >  1:1087368/1‑56 (MQ=255)
                                        aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                               <  1:5034452/71‑1 (MQ=255)
                                        aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                               >  1:610317/1‑71 (MQ=255)
                                        aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                               <  1:4415099/71‑1 (MQ=255)
                                        aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                               <  1:784231/71‑1 (MQ=255)
                                        aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                               <  1:5182046/71‑1 (MQ=255)
                                         tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg                                   <  1:4017068/66‑3 (MQ=255)
                                         tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg                                   <  1:4283486/66‑3 (MQ=255)
                                         tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg                                   <  1:1903804/66‑3 (MQ=255)
                                         tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc                              <  1:4345069/71‑1 (MQ=255)
                                         tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc                              <  1:114508/71‑1 (MQ=255)
                                         tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc                              <  1:1902831/71‑1 (MQ=255)
                                         tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc                              <  1:2494489/71‑1 (MQ=255)
                                          ttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAc                                                 >  1:1764189/1‑51 (MQ=255)
                                           tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGcc                                 >  1:3604451/1‑66 (MQ=255)
                                           tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg                             <  1:5217669/70‑1 (MQ=255)
                                           tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg                             <  1:2327593/70‑1 (MQ=255)
                                            cACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg                             <  1:1954216/69‑1 (MQ=255)
                                              cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGGCAGCGgag                          >  1:4861846/1‑70 (MQ=255)
                                              cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgag                          >  1:2485440/1‑70 (MQ=255)
                                               gACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCTTGCCAGCGgggac                        <  1:4978718/71‑2 (MQ=255)
                                                accaccAATGAAGAACTCTAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                                         <  1:5526757/53‑1 (MQ=255)
                                                 ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc                      >  1:1245625/1‑71 (MQ=255)
                                                   accaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa                        <  1:5295457/67‑1 (MQ=255)
                                                      gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa                                                  >  1:168162/2‑38 (MQ=25)
                                                      gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTg                 <  1:5022301/70‑1 (MQ=255)
                                                      actGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt                  <  1:5183383/68‑1 (MQ=255)
                                                      aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc                                 >  1:3819135/1‑55 (MQ=255)
                                                      aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc                                 >  1:5497285/1‑55 (MQ=255)
                                                       aTGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt                                                    <  1:1059066/35‑1 (MQ=25)
                                                       aTGAAGAATTCAAAACGGGCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGc                <  1:162148/71‑1 (MQ=255)
                                                         gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc                      <  1:1030113/63‑1 (MQ=255)
                                                         gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc                      <  1:3077125/63‑1 (MQ=255)
                                                          aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAAc                                                 >  1:3454373/1‑35 (MQ=255)
                                                          aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg             <  1:3482038/71‑1 (MQ=255)
                                                          aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg              >  1:2914009/1‑70 (MQ=255)
                                                           agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc                      <  1:4604216/61‑1 (MQ=255)
                                                            gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTac                                         <  1:3750045/41‑1 (MQ=19)
                                                            gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa            >  1:2674907/1‑70 (MQ=255)
                                                               ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcg                                     >  1:573003/2‑42 (MQ=16)
                                                               ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacg                                        <  1:188270/38‑1 (MQ=21)
                                                               ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacg                                        <  1:5389028/38‑1 (MQ=21)
                                                               ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                               <  1:1416983/47‑1 (MQ=19)
                                                               ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                               <  1:5411983/47‑1 (MQ=19)
                                                               ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg                               <  1:2235573/47‑1 (MQ=19)
                                                                tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg              >  1:4740757/1‑64 (MQ=255)
                                                                tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg              >  1:2266241/1‑64 (MQ=255)
                                                                tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTc       >  1:5697636/1‑71 (MQ=255)
                                                                 cAAAACGGTCGGTGATTTCCGGGTTAACATCATTacgg                                       >  1:5282043/1‑37 (MQ=16)
                                                                  aaaaCGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTccg     >  1:4684276/1‑69 (MQ=255)
                                                                  aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGa            >  1:1058383/1‑64 (MQ=255)
                                                                    aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa   <  1:4494449/71‑1 (MQ=255)
                                                                    aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa   <  1:5057228/71‑1 (MQ=255)
                                                                     gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc                                  >  1:2978820/1‑39 (MQ=255)
                                                                     gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc                                  >  1:1776076/1‑39 (MQ=255)
                                                                     gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc                                  >  1:766922/1‑39 (MQ=255)
                                                                     |                                                                      
CTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAA  >  minE/1933135‑1933274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A