Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,336 G→T 22.6% G369G (GGC→GGA)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,3360GT22.6% 270.7 / 52.2 119G369G (GGC→GGA) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (53/39);  new base T (15/12);  total (68/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 119 total aligned reads displayed.

TCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAG  >  minE/1933268‑1933402
                                                                    |                                                                  
tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc                                                                  >  1:3587386/1‑71 (MQ=255)
 ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc                                                                  >  1:228746/3‑70 (MQ=255)
 cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc                                                                  >  1:1606530/1‑70 (MQ=255)
  cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTccc                                                                 <  1:3444668/69‑1 (MQ=255)
  cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTccc                                                                 <  1:5349380/69‑1 (MQ=255)
  cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc                                                                  >  1:4073074/2‑69 (MQ=255)
  aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa                                                                <  1:3773351/71‑1 (MQ=255)
     aaTGAAGCTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc                                                             >  1:3738040/1‑71 (MQ=255)
      aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc                                                            >  1:2666226/1‑71 (MQ=255)
      aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc                                                            >  1:5092796/1‑71 (MQ=255)
      aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc                                                            >  1:3130815/1‑71 (MQ=255)
         aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa                                                                >  1:25551/1‑64 (MQ=255)
         aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc                                                           >  1:5525834/1‑69 (MQ=255)
         aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc                                                           >  1:1942019/1‑69 (MQ=255)
          aGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAg                                                        <  1:375750/71‑1 (MQ=255)
              tggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGcttt                                                    >  1:2315922/2‑69 (MQ=255)
               ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc                                                             <  1:4098982/61‑1 (MQ=255)
               ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc                                                             <  1:2444210/61‑1 (MQ=255)
               ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc                                                             <  1:545496/61‑1 (MQ=255)
               ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc                                                             <  1:58183/61‑1 (MQ=255)
               ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGctct                                                    <  1:3906577/70‑1 (MQ=255)
               ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCa                                                         >  1:1904783/1‑65 (MQ=255)
               ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCa                                                         >  1:3610316/1‑65 (MQ=255)
                   gAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGcttt                                                    >  1:3166104/1‑64 (MQ=255)
                    aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc                                                          >  1:1300250/1‑59 (MQ=255)
                    aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc                                                <  1:3421519/69‑1 (MQ=255)
                    aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa                                               <  1:3710205/70‑1 (MQ=255)
                    aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctcta                                              >  1:1322916/1‑69 (MQ=255)
                    aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc                                                <  1:1493536/69‑1 (MQ=255)
                    aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc                                                <  1:2721236/69‑1 (MQ=255)
                    aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc                                                <  1:1853951/69‑1 (MQ=255)
                     aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc                                                            <  1:3894561/56‑1 (MQ=255)
                     aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc                                                <  1:4173209/68‑1 (MQ=255)
                      tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc                                                          >  1:2997193/1‑57 (MQ=255)
                      tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc                                                          >  1:4681172/1‑57 (MQ=255)
                      tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc                                                          >  1:336830/1‑57 (MQ=255)
                      tCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAcc                                            >  1:4148008/1‑70 (MQ=255)
                       cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGccc                                                                 >  1:155765/1‑49 (MQ=255)
                       cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGccc                                                                 >  1:3603968/1‑49 (MQ=255)
                       cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa                                              >  1:1590314/1‑68 (MQ=255)
                       cAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc                                                >  1:50082/1‑66 (MQ=255)
                       cAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc                                                >  1:135385/1‑66 (MQ=255)
                        aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc                                                                  <  1:133440/47‑1 (MQ=255)
                        aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGt                                           <  1:2902844/70‑1 (MQ=255)
                        aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg                                          <  1:3297181/71‑1 (MQ=255)
                        aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg                                          >  1:4659103/1‑71 (MQ=255)
                        aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg                                          <  1:2546909/71‑1 (MQ=255)
                        aGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAccgt                                          <  1:3852109/71‑4 (MQ=255)
                           tgtgCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCa                                                                <  1:1279444/46‑1 (MQ=19)
                           tgtgCTTCTGCCACTTCATCAAAGATCTCTGTAACAATACGTCCCa                                                                <  1:4562239/46‑1 (MQ=25)
                            gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa                                                                >  1:1040531/1‑45 (MQ=255)
                            gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc                                                            >  1:1714676/1‑49 (MQ=255)
                             tgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg                                      >  1:1089477/1‑70 (MQ=255)
                              gCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa                                               <  1:3461882/60‑1 (MQ=255)
                               cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtcttc                                                  >  1:4239722/1‑56 (MQ=255)
                               cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg                                         >  1:347108/1‑65 (MQ=255)
                               cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc                                    >  1:3481753/1‑70 (MQ=255)
                               cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc                                    >  1:1034932/1‑70 (MQ=255)
                               cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc                                    >  1:2069040/1‑70 (MQ=255)
                                 tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa                                                                >  1:1984331/1‑40 (MQ=255)
                                 tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa                                                                >  1:2966395/1‑40 (MQ=255)
                                 tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa                                                                >  1:3955280/1‑40 (MQ=255)
                                  cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGCCCCCAGCtcttc                                                  >  1:2372027/1‑53 (MQ=255)
                                  cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa                                  <  1:2313055/69‑1 (MQ=255)
                                    gCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc                                                >  1:3801942/1‑53 (MQ=255)
                                        cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt                            >  1:5553026/1‑69 (MQ=255)
                                        cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt                            >  1:548779/1‑69 (MQ=255)
                                          tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg                         >  1:2793419/1‑70 (MQ=255)
                                          tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg                         >  1:296842/1‑70 (MQ=255)
                                           cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc                        <  1:3662298/70‑1 (MQ=255)
                                           cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc                        <  1:697422/70‑1 (MQ=255)
                                           cttcGAAGATCTAGGTAACGATACTGCCAAGACCCCAGct                                                      >  1:4581491/1‑40 (MQ=255)
                                            ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt                                       <  1:1923287/54‑1 (MQ=255)
                                            ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt                                       <  1:2489382/54‑1 (MQ=255)
                                             tcGAATATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt                     >  1:2191541/1‑71 (MQ=255)
                                             tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt                             <  1:2928213/63‑1 (MQ=255)
                                             tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt                     >  1:1137033/1‑71 (MQ=255)
                                             tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt                     >  1:693485/1‑71 (MQ=255)
                                              cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg                         >  1:3270839/1‑66 (MQ=255)
                                                aaGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGc                  <  1:5280438/71‑1 (MQ=255)
                                                 aGATCTCGGTAACGATACGGCCCAGACCCCAGCtctt                                                   >  1:2710813/1‑37 (MQ=255)
                                                  gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt                <  1:4023790/71‑1 (MQ=255)
                                                   aTCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCtt                >  1:2622580/1‑70 (MQ=255)
                                                        ggTAACGATACGGCCCAGACCCCAGCTCTTCTCaa                                              <  1:5103850/35‑1 (MQ=255)
                                                        ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc              <  1:476645/67‑1 (MQ=255)
                                                        ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc              <  1:1343296/67‑1 (MQ=255)
                                                        ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg             >  1:2012272/1‑68 (MQ=255)
                                                        ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg             >  1:3711795/1‑68 (MQ=255)
                                                        ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg             <  1:4826876/68‑1 (MQ=255)
                                                         gTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCaga         <  1:5367815/71‑1 (MQ=255)
                                                          tAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg                                          <  1:4947452/37‑1 (MQ=255)
                                                              gATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc                        <  1:2944620/51‑1 (MQ=255)
                                                              gATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa    <  1:5642619/71‑1 (MQ=255)
                                                               aTACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCt                 >  1:2604011/1‑57 (MQ=255)
                                                               aTACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa    <  1:5498546/70‑1 (MQ=255)
                                                                tACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGc            >  1:702205/1‑61 (MQ=255)
                                                                tACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg  <  1:3860992/71‑1 (MQ=255)
                                                                 aCGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCagcat       >  1:3577424/1‑62 (MQ=255)
                                                                 aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa           >  1:5191741/1‑61 (MQ=255)
                                                                 aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg  >  1:518802/1‑70 (MQ=255)
                                                                    |                                                                  
TCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAG  >  minE/1933268‑1933402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A