Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,693 A→G 34.4% G250G (GGT→GGC)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,6930AG34.4% 93.6 / 79.6 93G250G (GGT→GGC) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base G (21/11);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

TGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAT  >  minE/1933624‑1933763
                                                                     |                                                                      
tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac                                                                       >  1:2777119/1‑71 (MQ=255)
tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac                                                                       >  1:4228905/1‑71 (MQ=255)
tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac                                                                       >  1:1408252/1‑71 (MQ=255)
tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAAGGTTGATTTCGAATACACGCTCGAAGccac                                                                       >  1:2157608/1‑71 (MQ=255)
 gggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTACGAATACACGCTCGAAGccac                                                                       >  1:2774212/1‑70 (MQ=255)
  ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacc                                                                      <  1:4870123/70‑1 (MQ=255)
   gTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaa                                                                    >  1:2757253/1‑71 (MQ=255)
    ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgccta                                                                    >  1:5319967/1‑68 (MQ=255)
    ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgcct                                                                     >  1:2235956/1‑68 (MQ=255)
    ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctac                                                                   <  1:3838952/71‑1 (MQ=255)
      aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctacaa                                                                 >  1:748797/1‑69 (MQ=255)
       tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca                                                                        >  1:5042931/1‑63 (MQ=255)
       tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca                                                                        >  1:4435687/1‑63 (MQ=255)
       tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac                                                                <  1:3773230/71‑1 (MQ=255)
         aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca                                                              <  1:2818589/71‑1 (MQ=255)
             aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg                                                          >  1:1033978/1‑71 (MQ=255)
              cagAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgt                                                         <  1:4399028/69‑2 (MQ=255)
                gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGgcacc                                                                      >  1:421049/1‑56 (MQ=255)
                gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                       >  1:2675331/1‑71 (MQ=255)
                gAAATACCTTCATTACGGAAGTTATGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt                                                       >  1:3821128/1‑71 (MQ=255)
                 aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca                                                                        >  1:1239092/1‑53 (MQ=255)
                 aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTg                                                      <  1:4666974/71‑1 (MQ=255)
                  aaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGa                                                     <  1:2298181/71‑1 (MQ=255)
                   aTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt                                                       >  1:5186520/1‑68 (MQ=255)
                    tACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAga                                                   <  1:260610/71‑2 (MQ=255)
                    tACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAga                                                   <  1:5118977/71‑2 (MQ=255)
                      ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctacaa                                                                 >  1:3994674/1‑53 (MQ=255)
                      ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                 <  1:4856855/71‑1 (MQ=255)
                       cTTCGTTACGGAAGTTTCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc                                                <  1:3647018/71‑1 (MQ=255)
                       cTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc                                                <  1:187276/71‑1 (MQ=255)
                        ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacca                                                                     <  1:3893942/49‑1 (MQ=255)
                        ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacca                                                                     <  1:5360544/49‑1 (MQ=255)
                        ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGtg                                                 >  1:1564411/1‑68 (MQ=255)
                        ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTa                                                 >  1:604968/1‑69 (MQ=255)
                        ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                 >  1:2430177/1‑69 (MQ=255)
                          cattACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc                                             >  1:4430100/3‑70 (MQ=255)
                          cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca                                                                        >  1:1972426/1‑44 (MQ=255)
                          cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca                                                                        >  1:1274171/1‑44 (MQ=255)
                          cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAgg                                                   >  1:1963701/1‑65 (MQ=255)
                           attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgg                                             <  1:5207271/69‑2 (MQ=255)
                           attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc                                             <  1:1080444/69‑2 (MQ=255)
                           attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTA‑AACCAGACGTTTCAGATACAGCTc                                           >  1:2459265/2‑71 (MQ=255)
                              aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgcc                                                                      >  1:282983/1‑42 (MQ=19)
                              aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTcc                                          >  1:5351536/1‑70 (MQ=255)
                              aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCg                                         >  1:5008196/1‑71 (MQ=255)
                              aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAg                                                    >  1:421668/1‑60 (MQ=255)
                              aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc                                          <  1:2260078/70‑1 (MQ=255)
                              aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg                                         >  1:3371058/1‑71 (MQ=255)
                                  aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                 >  1:2971219/1‑59 (MQ=255)
                                  aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                 >  1:5252360/1‑59 (MQ=255)
                                  aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                 >  1:917003/1‑59 (MQ=255)
                                  aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                 >  1:5596705/1‑59 (MQ=255)
                                  aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg                                              >  1:1566166/1‑62 (MQ=255)
                                  aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                     <  1:2875600/71‑1 (MQ=255)
                                  aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCA‑CAACCACCAGACGCTTGAGGTACAg                                              >  1:1682360/1‑61 (MQ=255)
                                    gTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGa                                   <  1:4795269/71‑1 (MQ=255)
                                    gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                       <  1:4129044/51‑1 (MQ=255)
                                    gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                       <  1:515468/51‑1 (MQ=255)
                                    gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                       <  1:3189958/51‑1 (MQ=255)
                                      tACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATa                                 >  1:2469760/1‑71 (MQ=255)
                                       aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgt                                                         >  1:2275108/1‑45 (MQ=12)
                                       aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATa                                 >  1:4185953/1‑70 (MQ=255)
                                       aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATAc                                >  1:4862806/1‑71 (MQ=255)
                                       aCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                     >  1:5387034/1‑66 (MQ=255)
                                        cGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACg                               >  1:4656395/1‑71 (MQ=255)
                                         ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                              <  1:1084355/71‑1 (MQ=255)
                                         ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                              <  1:3248809/71‑1 (MQ=255)
                                         ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                              <  1:5289292/71‑1 (MQ=255)
                                           ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg                            >  1:3631369/1‑71 (MQ=255)
                                             gATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg                                              >  1:481697/1‑51 (MQ=255)
                                                ttCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa                       >  1:2757169/1‑71 (MQ=255)
                                                 tCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa                       >  1:490592/1‑70 (MQ=255)
                                                 tCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa                       >  1:3019741/1‑70 (MQ=255)
                                                  cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATg                     >  1:2840604/1‑71 (MQ=255)
                                                      tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                   >  1:4741747/1‑69 (MQ=255)
                                                        cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                   >  1:5660354/1‑67 (MQ=255)
                                                          cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc                        >  1:1303601/1‑60 (MQ=255)
                                                          cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc                        >  1:2664894/1‑60 (MQ=255)
                                                          cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc                        >  1:2671457/1‑60 (MQ=255)
                                                          cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc                        >  1:1057842/1‑60 (MQ=255)
                                                          cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga               <  1:3263218/69‑1 (MQ=255)
                                                          cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc             <  1:5599401/71‑1 (MQ=255)
                                                          cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc             <  1:2306737/71‑1 (MQ=255)
                                                           gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc            <  1:925502/71‑1 (MQ=255)
                                                            cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                              >  1:1373156/1‑52 (MQ=255)
                                                            cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                              >  1:1380682/1‑52 (MQ=255)
                                                            cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACaa                      >  1:362631/1‑59 (MQ=255)
                                                             tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc                                           <  1:4190127/38‑1 (MQ=255)
                                                             tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc                                           <  1:1940562/38‑1 (MQ=255)
                                                              cGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa                       >  1:935990/1‑57 (MQ=255)
                                                               gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCg                  <  1:446745/61‑1 (MQ=255)
                                                               gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg          <  1:1307289/69‑1 (MQ=255)
                                                                 aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg      >  1:3754388/1‑71 (MQ=255)
                                                                  gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt     >  1:1466483/1‑71 (MQ=255)
                                                                   ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                 <  1:2140913/58‑1 (MQ=255)
                                                                   ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                 <  1:307660/58‑1 (MQ=255)
                                                                    caccaACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                     >  1:2939465/1‑37 (MQ=255)
                                                                    caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                         >  1:252778/1‑49 (MQ=255)
                                                                     accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt  >  1:2121995/1‑71 (MQ=255)
                                                                     accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt  <  1:1891689/71‑1 (MQ=255)
                                                                     |                                                                      
TGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAT  >  minE/1933624‑1933763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A