Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,699 C→A 33.2% V248V (GTG→GTT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,6990CA33.2% 147.4 / 64.9 93V248V (GTG→GTT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (31/31);  new base A (20/11);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTG  >  minE/1933631‑1933767
                                                                    |                                                                    
tGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac                                                                    <  1:3773230/71‑1 (MQ=255)
  aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac                                                                    >  1:1326860/1‑69 (MQ=255)
  aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca                                                                  <  1:2818589/71‑1 (MQ=255)
   cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAg                                                                 <  1:1516433/71‑1 (MQ=255)
      aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg                                                              >  1:1033978/1‑71 (MQ=255)
       cagAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACgt                                                             <  1:4399028/69‑2 (MQ=255)
         gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                           >  1:2675331/1‑71 (MQ=255)
         gAAATACCTTCATTACGGAAGTTATGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt                                                           >  1:3821128/1‑71 (MQ=255)
           aaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGa                                                         <  1:2298181/71‑1 (MQ=255)
            aTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt                                                           >  1:5186520/1‑68 (MQ=255)
             tACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAga                                                       <  1:260610/71‑2 (MQ=255)
               ccTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                     <  1:4856855/71‑1 (MQ=255)
                cTTCGTTACGGAAGTTTCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc                                                    <  1:3647018/71‑1 (MQ=255)
                cTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc                                                    <  1:187276/71‑1 (MQ=255)
                 ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGtg                                                     >  1:1564411/1‑68 (MQ=255)
                 ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTa                                                     >  1:604968/1‑69 (MQ=255)
                 ttCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                     >  1:2430177/1‑69 (MQ=255)
                   cattACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc                                                 >  1:4430100/3‑70 (MQ=255)
                   cGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAgg                                                       >  1:1963701/1‑65 (MQ=255)
                    attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgg                                                 <  1:5207271/69‑2 (MQ=255)
                    attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc                                                 <  1:1080444/69‑2 (MQ=255)
                    attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAgc                                                 <  1:2252117/69‑2 (MQ=255)
                    attACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTA‑AACCAGACGTTTCAGATACAGCTc                                               >  1:2459265/2‑71 (MQ=255)
                       aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTcc                                              >  1:5351536/1‑70 (MQ=255)
                       aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCg                                             >  1:5008196/1‑71 (MQ=255)
                       aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAg                                                        >  1:421668/1‑60 (MQ=255)
                       aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTcc                                              <  1:2260078/70‑1 (MQ=255)
                       aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg                                             >  1:3371058/1‑71 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                     >  1:4202604/1‑59 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                     >  1:917003/1‑59 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                     >  1:5252360/1‑59 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                     >  1:5596705/1‑59 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATa                                                     >  1:2971219/1‑59 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg                                                  >  1:1566166/1‑62 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                         <  1:2875600/71‑1 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                         <  1:288199/71‑1 (MQ=255)
                           aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCA‑CAACCACCAGACGCTTGAGGTACAg                                                  >  1:1682360/1‑61 (MQ=255)
                             gTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGa                                       <  1:4795269/71‑1 (MQ=255)
                             gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                           <  1:515468/51‑1 (MQ=255)
                             gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                           <  1:3189958/51‑1 (MQ=255)
                             gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                           <  1:4129044/51‑1 (MQ=255)
                             gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCtt                                                           <  1:3097117/51‑1 (MQ=255)
                               tACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATa                                     >  1:2469760/1‑71 (MQ=255)
                                aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATa                                     >  1:4185953/1‑70 (MQ=255)
                                aCGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATAc                                    >  1:4862806/1‑71 (MQ=255)
                                 cGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACg                                   >  1:4656395/1‑71 (MQ=255)
                                  ggTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACG‑TTCAGATACAGCTCCGGCGCGATACGCa                                 <  1:201603/71‑1 (MQ=255)
                                  ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                  <  1:1084355/71‑1 (MQ=255)
                                  ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                  <  1:3931902/71‑1 (MQ=255)
                                  ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                  <  1:5289292/71‑1 (MQ=255)
                                  ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                  <  1:3248809/71‑1 (MQ=255)
                                    ttGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCa                                 <  1:665385/70‑1 (MQ=255)
                                    ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAg                                >  1:3631369/1‑71 (MQ=255)
                                      gATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg                                                  >  1:481697/1‑51 (MQ=255)
                                       aTTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATa                             <  1:4404712/71‑1 (MQ=255)
                                         ttCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa                           >  1:2757169/1‑71 (MQ=255)
                                          tCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa                           >  1:490592/1‑70 (MQ=255)
                                          tCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACa                           >  1:3019741/1‑70 (MQ=255)
                                          tCGAATACACGCTCGAAGCCACCAACCACCAGACGCt                                                            >  1:4662182/1‑37 (MQ=255)
                                          tCGAATACACGCTCGAAGCCACCAACCACCAGACGCt                                                            >  1:2582511/1‑37 (MQ=255)
                                           cGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATg                         >  1:2840604/1‑71 (MQ=255)
                                               tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                       >  1:4741747/1‑69 (MQ=255)
                                                 cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                       >  1:5660354/1‑67 (MQ=255)
                                                  acGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGAt                  <  1:2723919/71‑1 (MQ=255)
                                                   cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc                            >  1:1057842/1‑60 (MQ=255)
                                                   cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc                            >  1:1303601/1‑60 (MQ=255)
                                                   cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTAc                            >  1:2671457/1‑60 (MQ=255)
                                                   cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga                   <  1:3263218/69‑1 (MQ=255)
                                                   cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                 <  1:2306737/71‑1 (MQ=255)
                                                   cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                 <  1:5599401/71‑1 (MQ=255)
                                                    gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc                <  1:3558803/71‑1 (MQ=255)
                                                    gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc                <  1:925502/71‑1 (MQ=255)
                                                     cTCGAAGCCACCAACCACCTGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa               <  1:2373747/71‑1 (MQ=255)
                                                     cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                  >  1:2788999/1‑52 (MQ=255)
                                                     cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                  >  1:1373156/1‑52 (MQ=255)
                                                     cTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                  >  1:1380682/1‑52 (MQ=255)
                                                      tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc                                               <  1:1940562/38‑1 (MQ=255)
                                                       cGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa                           >  1:935990/1‑57 (MQ=255)
                                                        gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCg                      <  1:446745/61‑1 (MQ=255)
                                                        gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg              <  1:1307289/69‑1 (MQ=255)
                                                          aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg          >  1:3754388/1‑71 (MQ=255)
                                                           gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt         <  1:5160679/71‑1 (MQ=255)
                                                           gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt         >  1:1466483/1‑71 (MQ=255)
                                                           gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt         <  1:530615/71‑1 (MQ=255)
                                                            ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                     <  1:307660/58‑1 (MQ=255)
                                                            ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                     <  1:2140913/58‑1 (MQ=255)
                                                             caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                             >  1:252778/1‑49 (MQ=255)
                                                              accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt      <  1:1891689/71‑1 (MQ=255)
                                                              accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt      >  1:2121995/1‑71 (MQ=255)
                                                                caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg    <  1:5127902/71‑1 (MQ=255)
                                                                caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATACAGCGCGTTAtgg    <  1:2083800/71‑1 (MQ=255)
                                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGa                                       >  1:5087035/1‑35 (MQ=255)
                                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACg                                   <  1:149809/39‑1 (MQ=255)
                                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACg                                   <  1:1845592/39‑1 (MQ=255)
                                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACg                                   <  1:4778200/39‑1 (MQ=255)
                                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgc             >  1:1710353/1‑61 (MQ=255)
                                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgc             >  1:3788737/1‑61 (MQ=255)
                                                                 aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt   >  1:768292/1‑71 (MQ=255)
                                                                  accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg  <  1:4447946/71‑1 (MQ=255)
                                                                    caccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                     >  1:1355163/1‑50 (MQ=255)
                                                                    |                                                                    
TGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTG  >  minE/1933631‑1933767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A