Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,747 G→T 22.0% L232L (CTC→CTA) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,7470GT22.0% 285.0 / 49.7 118L232L (CTC→CTA) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (32/60);  new base T (17/9);  total (49/69)
Fisher's exact test for biased strand distribution p-value = 6.84e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

ACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCG  >  minE/1933681‑1933817
                                                                  |                                                                      
acGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGAt                                                                    <  1:2723919/71‑1 (MQ=255)
 cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga                                                                     <  1:3263218/69‑1 (MQ=255)
 cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                                                                   <  1:2306737/71‑1 (MQ=255)
 cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                                                                   <  1:5599401/71‑1 (MQ=255)
  gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc                                                                  <  1:3558803/71‑1 (MQ=255)
  gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc                                                                  <  1:925502/71‑1 (MQ=255)
      gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCg                                                                        <  1:446745/61‑1 (MQ=255)
      gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg                                                                <  1:1307289/69‑1 (MQ=255)
        aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                            >  1:97304/1‑71 (MQ=255)
        aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                            >  1:3754388/1‑71 (MQ=255)
         gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                           <  1:5160679/71‑1 (MQ=255)
          ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                                                                       <  1:2140913/58‑1 (MQ=255)
          ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                                                                       <  1:307660/58‑1 (MQ=255)
            accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                        >  1:2121995/1‑71 (MQ=255)
               aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgc                                                               >  1:3788737/1‑61 (MQ=255)
               aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                     >  1:768292/1‑71 (MQ=255)
                accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                                    <  1:4447946/71‑1 (MQ=255)
                accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                      >  1:1444606/1‑69 (MQ=255)
                acaaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                    >  1:4321830/4‑71 (MQ=255)
                acaaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                    >  1:4548195/4‑71 (MQ=255)
                 caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                                   >  1:1068133/3‑71 (MQ=255)
                  caccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                                                                       >  1:1355163/1‑50 (MQ=255)
                   accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                 <  1:2784532/71‑1 (MQ=255)
                   accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                  >  1:3398683/1‑70 (MQ=255)
                   accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                  >  1:829866/1‑70 (MQ=255)
                   accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                 <  1:3568469/71‑1 (MQ=255)
                   accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                 >  1:5677978/1‑71 (MQ=255)
                    ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                 >  1:1133008/1‑70 (MQ=255)
                    ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                 >  1:739177/1‑70 (MQ=255)
                    ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                  >  1:4618509/1‑69 (MQ=255)
                     caGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                            <  1:1430205/58‑1 (MQ=255)
                      aGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                               >  1:1790977/1‑70 (MQ=255)
                       gACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAAt                                              >  1:3131719/1‑70 (MQ=255)
                       gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                             <  1:585195/71‑1 (MQ=255)
                        aCGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaataa                                            <  1:1714656/71‑1 (MQ=255)
                          gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                          >  1:5113055/1‑71 (MQ=255)
                          gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                          <  1:4374755/71‑1 (MQ=255)
                              gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                        <  1:5619305/69‑1 (MQ=255)
                              gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                        <  1:4481914/69‑1 (MQ=255)
                              gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                        <  1:743625/69‑1 (MQ=255)
                               agatACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcg                                     <  1:4996120/68‑1 (MQ=255)
                                 gTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                        <  1:752053/50‑1 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCATATACATATCTAAATCCAGAGCATTAt                                                        <  1:280149/49‑1 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAt                                                        <  1:4654202/49‑1 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                   >  1:4868777/2‑69 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                   >  1:5531915/2‑69 (MQ=255)
                                  tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                   >  1:5297841/1‑70 (MQ=255)
                                  tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                  <  1:3213693/71‑1 (MQ=255)
                                   aCAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                      >  1:1514538/1‑50 (MQ=255)
                                   aCAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                   >  1:4957127/1‑69 (MQ=255)
                                    cAGTTCCGGTGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCa                                 <  1:2054571/70‑1 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                 <  1:1493471/38‑1 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                           >  1:5344057/1‑60 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                <  1:4187216/71‑1 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                <  1:2744063/71‑1 (MQ=255)
                                    cAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                 <  1:1520136/70‑1 (MQ=255)
                                    cAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                 <  1:3098146/70‑1 (MQ=255)
                                      gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                 <  1:2269693/36‑1 (MQ=255)
                                      gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                 <  1:5223067/36‑1 (MQ=255)
                                      gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                              >  1:2763614/1‑71 (MQ=255)
                                      gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                              <  1:1366105/71‑1 (MQ=255)
                                       ttCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                             <  1:3277419/55‑1 (MQ=255)
                                       ctCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                 <  1:2434266/66‑1 (MQ=255)
                                        tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                             >  1:3631966/1‑70 (MQ=255)
                                        tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                             >  1:1682187/1‑70 (MQ=255)
                                          cGGCGCGATTCGCAGGTACATGTCGAGATCCAGCGCGt                                                           <  1:1514816/38‑1 (MQ=255)
                                          cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                           <  1:4096482/38‑1 (MQ=255)
                                          cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAc                          >  1:37593/1‑71 (MQ=255)
                                          cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgg                                                      >  1:5159091/1‑43 (MQ=16)
                                            gcgcGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAc                                      <  1:2283399/57‑1 (MQ=255)
                                             cgcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGCTAAACGGACGCGCAGCGGCACCGc                       <  1:5365137/71‑1 (MQ=255)
                                                gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                    <  1:1173550/71‑1 (MQ=255)
                                                gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                    <  1:1766010/71‑1 (MQ=255)
                                                gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                    <  1:1631914/71‑1 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                             <  1:1623944/45‑1 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                         <  1:886589/49‑1 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                         <  1:2549212/49‑1 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                   >  1:4510568/1‑71 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                   >  1:2634568/1‑71 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                   >  1:3536593/1‑71 (MQ=255)
                                                  tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                               >  1:5393793/1‑42 (MQ=255)
                                                  tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                               >  1:4060995/1‑42 (MQ=255)
                                                  tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                      <  1:3841223/67‑1 (MQ=255)
                                                  tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAggg                  <  1:182418/71‑1 (MQ=255)
                                                    cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa                     >  1:2929089/1‑66 (MQ=255)
                                                    cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                 <  1:3943651/70‑1 (MQ=255)
                                                     gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa                           <  1:211022/59‑1 (MQ=255)
                                                      cAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCa              <  1:2229378/71‑1 (MQ=255)
                                                       aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc             <  1:1902217/71‑1 (MQ=255)
                                                        ggTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                         <  1:167878/42‑1 (MQ=255)
                                                         gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt           <  1:2411358/71‑1 (MQ=255)
                                                          tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                  <  1:4856365/47‑1 (MQ=255)
                                                          tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTg          >  1:3909127/1‑71 (MQ=255)
                                                            cATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                          <  1:196368/37‑1 (MQ=255)
                                                             aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat       >  1:3289528/1‑71 (MQ=255)
                                                             aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGca        >  1:5083439/1‑70 (MQ=255)
                                                              tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatc      <  1:2953586/71‑1 (MQ=255)
                                                                tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                      <  1:2705047/53‑1 (MQ=255)
                                                                  gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt           >  1:2118789/1‑62 (MQ=255)
                                                                  gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCg  >  1:1910079/1‑71 (MQ=255)
                                                                  |                                                                      
ACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCG  >  minE/1933681‑1933817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A