Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,934,002 G→A 32.3% T147T (ACC→ACT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,934,0020GA32.3% 111.2 / 78.2 96T147T (ACC→ACT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (25/40);  new base A (13/18);  total (38/58)
Fisher's exact test for biased strand distribution p-value = 8.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

GCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCG  >  minE/1933935‑1934072
                                                                   |                                                                      
gttGACGATAACGGACTTCCTGATCCTGCAGACCATGGGATTTATCTGGTAAAGGACGTAGTGCTTTAGTc                                                                     <  1:880468/69‑1 (MQ=255)
   gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                                                   >  1:1287451/1‑70 (MQ=255)
   gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                                                   >  1:460533/1‑70 (MQ=255)
   gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                                                   <  1:408624/70‑1 (MQ=255)
    aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagca                                                                 <  1:871873/71‑1 (MQ=255)
    aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagca                                                                 <  1:5361150/71‑1 (MQ=255)
    aCGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcagc                                                                  <  1:4004532/70‑1 (MQ=255)
          gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc                                                            <  1:3250009/70‑1 (MQ=255)
            gcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAAc                                                         >  1:3602980/1‑71 (MQ=255)
             gacTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt                                                        <  1:3181762/69‑1 (MQ=255)
             gacTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt                                                        <  1:4835933/69‑1 (MQ=255)
             cgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                                                      >  1:2058026/1‑57 (MQ=255)
             cgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                                        >  1:1744131/1‑71 (MQ=255)
              gcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTc                                                       <  1:3085453/71‑1 (MQ=255)
              acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                                                   <  1:161760/58‑1 (MQ=255)
              acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag                                                                   <  1:3137036/58‑1 (MQ=255)
               cTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCACCt                                                        >  1:1213546/1‑69 (MQ=255)
                ttCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgcgc                                                            <  1:3471179/64‑1 (MQ=255)
                ttCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgcgc                                                            <  1:4853049/64‑1 (MQ=255)
                  ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTcag                                                     <  1:1144142/69‑3 (MQ=255)
                  ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGta                                                   >  1:5676856/1‑70 (MQ=255)
                  ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGAc                                                              >  1:94270/1‑60 (MQ=255)
                   cTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTca                                                      >  1:4077343/1‑66 (MQ=255)
                   cTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGtac                                                  <  1:4136137/71‑3 (MQ=255)
                   cTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGtac                                                  <  1:1974970/71‑3 (MQ=255)
                   cTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGta                                                   <  1:4003635/70‑2 (MQ=255)
                    tGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCa                                                           >  1:2312386/1‑61 (MQ=255)
                    tGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                                                   >  1:285824/1‑69 (MQ=255)
                      aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                               <  1:4825226/71‑1 (MQ=255)
                      aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTgg                                                                       <  1:2292799/47‑1 (MQ=255)
                      aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGGGCAGt                                               >  1:1274071/1‑71 (MQ=255)
                      aTCCTGCAAGCCGTGGAATTTATACGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                                               >  1:2917538/1‑71 (MQ=255)
                       tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                               >  1:4916423/1‑70 (MQ=255)
                       tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                               >  1:2325026/1‑70 (MQ=255)
                       tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                               >  1:3631352/1‑70 (MQ=255)
                       tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTg                                              <  1:4174745/71‑1 (MQ=255)
                       tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg                                              <  1:1761591/71‑1 (MQ=255)
                       tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg                                              <  1:3500613/71‑1 (MQ=255)
                       tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGAGCAGTg                                              <  1:735382/71‑1 (MQ=255)
                                ccGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                     <  1:4668860/71‑1 (MQ=255)
                                 catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc                                    >  1:4588118/3‑70 (MQ=255)
                                 catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc                                    >  1:2573148/3‑70 (MQ=255)
                                 catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc                                    >  1:1855730/3‑70 (MQ=255)
                                 cGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACa                                      <  1:4822578/69‑1 (MQ=255)
                                  atGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTg                                              <  1:1258198/59‑1 (MQ=255)
                                   tGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgcgc                                                            <  1:3170555/45‑1 (MQ=255)
                                   tGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTc                                  <  1:3823357/71‑1 (MQ=255)
                                   tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg                                                      <  1:2863152/51‑1 (MQ=255)
                                    ggAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGa                                        >  1:2926615/1‑64 (MQ=255)
                                     gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGc                                <  1:191712/71‑1 (MQ=255)
                                      aaTTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTc                                                       <  1:386645/47‑1 (MQ=255)
                                        tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                                        <  1:5386285/44‑1 (MQ=255)
                                        tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                                        <  1:2585115/44‑1 (MQ=255)
                                        tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                                                        <  1:3768983/44‑1 (MQ=255)
                                        tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTgg                                             <  1:3767021/55‑1 (MQ=255)
                                        tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCgg                             >  1:2880493/1‑71 (MQ=255)
                                         ttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc                                       <  1:2890008/60‑1 (MQ=255)
                                          tATCCCGCAGCGGACGCAGTGCTTTGGTCAGCAGACg                                                             >  1:4659781/1‑37 (MQ=255)
                                              cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg                       <  1:5295937/71‑1 (MQ=255)
                                              cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg                       <  1:3564349/71‑1 (MQ=255)
                                                gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTc                                  <  1:4178431/58‑1 (MQ=255)
                                                gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt                     >  1:1499819/1‑71 (MQ=255)
                                                gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt                     >  1:5415451/1‑71 (MQ=255)
                                                  aGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt                         >  1:4613305/1‑65 (MQ=255)
                                                   gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                                        <  1:1941728/49‑1 (MQ=255)
                                                   gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                                        <  1:861621/49‑1 (MQ=255)
                                                   gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                                        <  1:4663411/49‑1 (MQ=255)
                                                   gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg                  <  1:215619/71‑1 (MQ=255)
                                                   gCGGACGCAGTGCTTTGGGCAGCAGACGCAACTCGGTGCAGTGGATAGa                                        <  1:3394994/49‑1 (MQ=255)
                                                    cGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGa                 <  1:5582937/71‑1 (MQ=255)
                                                      gACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAAc               <  1:4641451/71‑1 (MQ=255)
                                                       aCGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGaa                >  1:2462441/1‑69 (MQ=255)
                                                          cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                     <  1:3005811/45‑1 (MQ=255)
                                                          cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                     <  1:4849127/45‑1 (MQ=255)
                                                          cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                     <  1:1403694/45‑1 (MQ=255)
                                                          cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                     <  1:3062072/45‑1 (MQ=255)
                                                            gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc                                       <  1:3277571/41‑1 (MQ=255)
                                                            gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGt                                    <  1:5414204/44‑1 (MQ=255)
                                                            gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGt                                    <  1:5187435/44‑1 (MQ=255)
                                                            gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGc            <  1:5055446/68‑1 (MQ=255)
                                                            gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGc            <  1:1988341/68‑1 (MQ=255)
                                                             tGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtt                           <  1:2294863/52‑1 (MQ=255)
                                                              gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc       >  1:854649/1‑71 (MQ=255)
                                                              gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc       >  1:4893703/1‑71 (MQ=255)
                                                              gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc       >  1:4105097/1‑71 (MQ=255)
                                                              gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc       >  1:47924/1‑71 (MQ=255)
                                                              gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc       >  1:1074442/1‑71 (MQ=255)
                                                              gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAc        >  1:1799409/1‑70 (MQ=255)
                                                               cTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                     >  1:5202966/1‑40 (MQ=255)
                                                               cTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                     >  1:1796875/1‑40 (MQ=255)
                                                               cTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCt      >  1:5323365/1‑71 (MQ=255)
                                                                 ttGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                                        <  1:2630518/35‑1 (MQ=255)
                                                                  tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACa              >  1:591886/1‑60 (MQ=255)
                                                                  tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCttt    <  1:2978676/70‑1 (MQ=255)
                                                                  tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCtt     <  1:4657059/69‑1 (MQ=255)
                                                                  tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTc   >  1:5371789/1‑71 (MQ=255)
                                                                  tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTc   >  1:2457260/1‑71 (MQ=255)
                                                                   ggTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTcg  >  1:1529964/1‑71 (MQ=255)
                                                                   |                                                                      
GCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCG  >  minE/1933935‑1934072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A