Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,142,814 C→T Q312Q (CAG→CAA)  asmA ← predicted assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,142,8140CT95.0% 244.7 / 0.6 99Q312Q (CAG→CAA) asmApredicted assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base T (94/0);  total (94/5)
Fisher's exact test for biased strand distribution p-value = 1.40e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 124 total aligned reads displayed.

GGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAG  >  W3110S.gb/2142786‑2142864
                            |                                                  
ggCGCGGCAACGTTGATTGGCTCTGCCCTtgctgc                                              >  1:129374/1‑35 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTgcgc                                            >  1:1192404/1‑37 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTgcgc                                            >  1:88183/1‑37 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCgg                                          >  1:709098/1‑39 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCTGCACCGTTCTCACCGTTCGCTGt                    >  1:241975/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCCCCGTTCTCACCGTTCGCTGt                    >  1:948447/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCAcc                             >  1:537274/1‑52 (MQ=39)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg                     >  1:764676/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg                     >  1:387688/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg                     >  1:1253271/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg                     >  1:522655/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg                     >  1:1070711/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg                     >  1:92507/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgctg                     >  1:146469/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgct                      >  1:1091515/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTcgc                       >  1:1052412/1‑58 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:293364/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:800887/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:265200/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:852649/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:326377/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:252526/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:381014/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:24259/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:427836/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:908991/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:429365/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:454994/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:735802/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:488515/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:489372/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:510954/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:52945/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:791211/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:618871/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:636359/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:692362/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:693340/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:705267/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:729353/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:729820/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:754312/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:981995/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:959890/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1134899/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1119112/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1105177/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1101798/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1100553/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1094153/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:108963/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1470036/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1058118/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1051396/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1046428/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1029225/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1015412/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1015116/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:985917/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:956311/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:166484/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1467305/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:909452/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1408605/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1378298/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1347324/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1308625/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1291713/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:931188/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1252538/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1233993/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1233901/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1201575/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:1164886/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTAACCGTTCGCTGt                    >  1:374771/1‑61 (MQ=255)
ggCGCGGCAACGTTGATTGGCTCAGCCCTTGCTGCGCGGCACCGTTCTCACCGTTc                         >  1:683260/1‑56 (MQ=255)
ggCGCGGCAACGTTGATTAGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:843263/1‑61 (MQ=255)
ggCGCGGCAACCTTGATTGGCTCTGCCCTTGCTGCGCGGCACCGTTCTCACCGTTCGCTGt                    >  1:968579/1‑61 (MQ=255)
                  ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGTGGTTTCATTAAGCg         <  1:32120/54‑1 (MQ=255)
                  ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg         <  1:1147762/54‑1 (MQ=255)
                  ggCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCg         <  1:48673/54‑1 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCCTTCGCGGTTTCATTAAGCGGGATGa   >  1:1387205/1‑53 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCgg                     >  1:1435050/1‑35 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:217697/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:10140/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:107095/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:1122605/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:1201128/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:1207756/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:1254569/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:918206/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:732154/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:907943/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:300461/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:101051/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:446590/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:791205/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:777497/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAg  >  1:616865/1‑54 (MQ=255)
                         ccccTGCTGCGCGGCACCGTTTTCACAATTCGCGGTTTCATTAAGCGGGATGAg  >  1:53255/1‑54 (MQ=255)
                            |                                                  
GGCGCGGCAACGTTGATTGGCTCTGCCCCTGCTGCGCGGCACCGTTTTCACCATTCGCGGTTTCATTAAGCGGGATGAG  >  W3110S.gb/2142786‑2142864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A