Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,151,914 |
T→C |
A403A (GCA→GCG) |
yegI ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,151,914 | 0 | T | C | 100.0%
| 162.9
/ NA
| 58 | A403A (GCA→GCG) | yegI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (58/0); total (58/0) |
AGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGC > W3110S.gb/2151906‑2151963
|
agcGATAACTCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:495910/1‑58 (MQ=255)
agcGATAACGCTGACTGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1146957/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTcg > 1:1105617/1‑45 (MQ=38)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAAc > 1:500353/1‑54 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAgg > 1:456241/1‑57 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAgg > 1:457841/1‑57 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAgg > 1:77312/1‑57 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:56952/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:625175/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:556432/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:519627/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:496764/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:488317/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:463526/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:457174/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:441418/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:395399/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:62200/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:322535/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:631717/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:647489/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:688998/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:74613/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:771109/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:824607/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:84456/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:887226/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:925898/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:973640/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:981956/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:134913/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1077683/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1081818/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:108464/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1087992/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1106534/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1110657/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:11316/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1185545/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1205190/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:12235/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1238938/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1267517/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1339462/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:366680/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1356642/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1417549/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1435196/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1445673/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1446191/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1461277/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:148185/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:153131/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:158688/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:237881/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:269075/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:279741/1‑58 (MQ=255)
agcGATAACGCTGACAGTGCGATCTCAATCAGAATGATGTATTCGCGCCGTAACAGGc > 1:1011740/1‑58 (MQ=255)
|
AGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGC > W3110S.gb/2151906‑2151963
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
