Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 790,237 A→G S55S (AGT→AGC)  galK ← galactokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb790,2370AG100.0% 207.4 / NA 106S55S (AGT→AGC) galKgalactokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/106);  total (0/106)

Only 100 of 106 total aligned reads displayed.

CATCGCGTGGTGCACAACTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACCG  >  W3110S.gb/790221‑790282
                |                                             
cATCTCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:78569/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:60927/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:67127/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:66790/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:66705/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:66237/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:661/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:65144/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:64970/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:649/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:63852/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:62541/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:62252/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:61027/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:67554/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:6048/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:60158/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:56701/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:56622/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:55891/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:55883/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:55245/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:54648/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:52793/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:52702/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:52261/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:760/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:8757/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:85853/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:85834/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:85709/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:85352/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:82927/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:82361/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:80191/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:79993/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:79340/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:78256/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:52172/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:75013/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:74501/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:74202/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:74147/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:72435/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:69368/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:69343/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:68867/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:68795/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:68505/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:68211/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:22733/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:3216/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:30743/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:29949/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:29691/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:28953/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:28585/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:2793/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:27306/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:26201/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:24949/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:2428/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:50722/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:21995/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:21968/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:21511/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:1997/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:19937/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:18424/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:18281/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:17831/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:17468/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:16784/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:16537/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:43644/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:10979/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:50538/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:49797/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:49503/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:49229/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:4805/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:46836/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:45744/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:45464/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:4462/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:44439/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:32196/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:41741/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:41657/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:40768/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:39947/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:38281/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:3739/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:36477/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:3478/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:34730/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:34059/62‑1 (MQ=255)
cATCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:32844/62‑1 (MQ=255)
 aTCGCGTGGCGCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:14169/61‑1 (MQ=255)
          cgCACAGCTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACcg  <  1:22267/51‑1 (MQ=255)
                |                                             
CATCGCGTGGTGCACAACTGATCACGGTTTGATAATCAATCGCGCAGGGCAGAACGAAACCG  >  W3110S.gb/790221‑790282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A