Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,169,249 |
+T |
intergenic (+83/+190) |
yegQ → / ← ogrK |
predicted peptidase/DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,169,246 | 1 | . | T | 100.0%
| 13.5
/ NA
| 6 | intergenic (+80/+193) | yegQ/ogrK | predicted peptidase/DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (6/0); total (6/0) |
ATTTATTAAGGTTATCATCCG‑TTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACC > W3110S.gb/2169226‑2169286
|
aTTTATTAAGGTTATCATCCGTTTTCGCTGAAAAACATAACCCAt > 1:2016058/1‑45 (MQ=38)
aTTTATTAAGGTTATCATCCGTTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTAcc > 1:1439237/1‑62 (MQ=255)
aTTTATTAAGGTTATCATCCGTTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTAcc > 1:1467859/1‑62 (MQ=255)
aTTTATTAAGGTTATCATCCGTTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTAcc > 1:1504524/1‑62 (MQ=255)
aTTTATTAAGGTTATCATCCGTTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTAcc > 1:1897255/1‑62 (MQ=255)
aTTTATTAAGGTTATCATCCGTTTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTAcc > 1:324339/1‑62 (MQ=255)
|
ATTTATTAAGGTTATCATCCG‑TTTCGCTGAAAAACATAACCCATAAAATGCTAGCTGTACC > W3110S.gb/2169226‑2169286
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
