Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F17 I0 R1 67 74.8 3863507 93.7% 3620106 61.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,892,0191.G100.0% 187.8 / NA 52intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/26);  total (26/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*minE1,892,0192.C100.0% 187.9 / NA 52intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/26);  total (26/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  minE/1891961‑1892077
                                                           ||                                                          
ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:758274/71‑1 (MQ=255)
ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:1689156/71‑1 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               >  1:2820272/1‑70 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               <  1:475012/70‑1 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                <  1:3249877/69‑1 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                <  1:3795687/69‑1 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                                              >  1:1066714/1‑71 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                                              >  1:1801551/1‑71 (MQ=255)
    aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                                              >  1:1720669/1‑71 (MQ=255)
     aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg                                             >  1:3540778/1‑71 (MQ=255)
        gattcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc                                          <  1:3834663/71‑1 (MQ=255)
         attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         <  1:2543757/71‑1 (MQ=255)
           tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:1087296/1‑71 (MQ=255)
           tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:2335846/1‑71 (MQ=255)
              attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:3302031/71‑1 (MQ=255)
                  aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc                                 >  1:1256370/1‑70 (MQ=255)
                    cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  <  1:866142/67‑1 (MQ=255)
                    cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt                              >  1:251509/1‑71 (MQ=255)
                      ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg                                        <  1:2954944/59‑1 (MQ=255)
                       cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg                                             >  1:2944972/1‑53 (MQ=255)
                        gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca                                                    <  1:2126453/45‑1 (MQ=255)
                        gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca                                                    <  1:1665679/45‑1 (MQ=255)
                        gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgcg                          >  1:3166837/1‑71 (MQ=255)
                         cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         >  1:1365911/1‑71 (MQ=255)
                          tAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:385502/1‑56 (MQ=255)
                          tAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     >  1:153901/1‑58 (MQ=255)
                          tAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     >  1:2946047/1‑58 (MQ=255)
                           aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:8153/44‑1 (MQ=255)
                           aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:991163/44‑1 (MQ=255)
                            aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       <  1:603452/70‑1 (MQ=255)
                              aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt                    >  1:430574/1‑71 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGaa                                                 >  1:663535/1‑41 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:1455040/1‑42 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:2140285/1‑42 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:2131643/1‑42 (MQ=255)
                               cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:2023354/1‑42 (MQ=255)
                                gACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATcc                  <  1:1662974/71‑1 (MQ=255)
                                 aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATATGACGTTCGCGTCTCATCCg                 <  1:3107286/71‑1 (MQ=255)
                                 aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg                 <  1:2535161/71‑1 (MQ=255)
                                   aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:1515447/50‑1 (MQ=255)
                                   aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:1225412/50‑1 (MQ=255)
                                      tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg                 >  1:920476/1‑66 (MQ=255)
                                      tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt             >  1:2392108/1‑70 (MQ=255)
                                          gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg        <  1:805717/71‑1 (MQ=39)
                                           ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGAc               >  1:1172239/1‑63 (MQ=38)
                                           ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt       >  1:438246/1‑71 (MQ=255)
                                              gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt     <  1:3809039/70‑1 (MQ=38)
                                              gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt     <  1:2146530/70‑1 (MQ=38)
                                               ctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       <  1:2801588/51‑1 (MQ=255)
                                                tGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  >  1:1737316/1‑39 (MQ=25)
                                                  aaCATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTg            <  1:3447525/59‑1 (MQ=38)
                                                  aaCATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAc  <  1:104575/69‑1 (MQ=38)
                                                           ||                                                          
CCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  minE/1891961‑1892077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A