Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
833,307 |
G→T |
T22T (ACC→ACA) |
ybiA ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 833,307 | 0 | G | T | 100.0%
| 85.7
/ NA
| 27 | T22T (ACC→ACA) | ybiA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (8/19); total (8/19) |
AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGA > W3110S.gb/833256‑833364
|
aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa < 1:500352/62‑1 (MQ=255)
aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa < 1:3194274/62‑1 (MQ=255)
aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa < 1:439265/62‑1 (MQ=255)
aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa < 1:2002272/62‑1 (MQ=255)
aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa < 1:192514/62‑1 (MQ=255)
gggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTatg < 1:4012691/61‑1 (MQ=255)
gggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTatg < 1:3816578/61‑1 (MQ=255)
ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat < 1:704739/59‑1 (MQ=255)
ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat < 1:4196505/59‑1 (MQ=255)
ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat < 1:3227061/59‑1 (MQ=255)
ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat < 1:1657342/59‑1 (MQ=255)
cgaGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg < 1:1873811/60‑1 (MQ=255)
ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg < 1:4617793/62‑1 (MQ=255)
ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg < 1:1076099/62‑1 (MQ=255)
ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg < 1:3992884/62‑1 (MQ=255)
ccAGGCGGCAAAGTTAGAAAAATCACCGTAGGCATCGCTTGTGCTGTAGAAATTTatgattg < 1:2160294/62‑3 (MQ=255)
ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt > 1:638065/1‑59 (MQ=255)
ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt > 1:1064228/1‑59 (MQ=255)
ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt > 1:1685004/1‑59 (MQ=255)
ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt > 1:1618262/1‑59 (MQ=255)
ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt > 1:1413622/1‑59 (MQ=255)
tCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAt > 1:3917655/1‑62 (MQ=255)
tCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAt > 1:163427/1‑62 (MQ=255)
aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTc < 1:3784683/62‑1 (MQ=255)
aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTc < 1:1057299/62‑1 (MQ=255)
aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTc < 1:767712/62‑1 (MQ=255)
cGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGa > 1:782809/1‑62 (MQ=255)
|
AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGA > W3110S.gb/833256‑833364
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
