Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,870,366 |
2 bp→GC |
coding (1745‑1746/1935 nt) |
yeaG → |
conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,870,366 | 0 | C | G | 91.7%
| 32.0
/ ‑3.4
| 12 | T582S (ACG→AGC) | yeaG | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (11/0); total (11/1) |
| Fisher's exact test for biased strand distribution p-value = 8.33e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 |
| * | W3110S.gb | 1,870,367 | 0 | G | C | 91.7%
| 32.6
/ ‑3.2
| 12 | T582S (ACG→AGC) | yeaG | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (11/0); total (11/1) |
| Fisher's exact test for biased strand distribution p-value = 8.33e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 |
CGCGCCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAAC > W3110S.gb/1870305‑1870427
||
cgcgcCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCAc > 1:552941/1‑62 (MQ=255)
aaTCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCaa < 1:2495472/62‑1 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTtctcga > 1:2782153/1‑50 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:125380/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:170733/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:1978902/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:2134898/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:2510989/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:2763785/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:2783941/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:3098013/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:416443/1‑53 (MQ=255)
tGGACCAGCTACGAGAAGTTCCGCAGCGTGATCGAGAAGAAGATGTTCTCGaa > 1:612996/1‑53 (MQ=255)
ggTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAAc > 1:1713915/1‑61 (MQ=255)
||
CGCGCCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAAC > W3110S.gb/1870305‑1870427
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
