Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,872,787 |
C→T |
P230L (CCG→CTG) |
yeaI → |
predicted diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,872,787 | 0 | C | T | 100.0%
| 24.6
/ NA
| 9 | P230L (CCG→CTG) | yeaI | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (9/0); total (9/0) |
TGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCA > W3110S.gb/1872755‑1872816
|
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:1135751/1‑62 (MQ=255)
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:1415526/1‑62 (MQ=255)
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:1475586/1‑62 (MQ=255)
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:1593749/1‑62 (MQ=255)
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:1843738/1‑62 (MQ=255)
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:208131/1‑62 (MQ=255)
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:2510785/1‑62 (MQ=255)
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:33994/1‑62 (MQ=255)
tGACTATTCTTTGTATGTCGTCGATTACTGTCTGGACAACCATACTGCGACCTGGGGAATCa > 1:459661/1‑62 (MQ=255)
|
TGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCA > W3110S.gb/1872755‑1872816
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
