Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,208,601 |
G→T |
T224T (ACG→ACT) |
yehL → |
predicted transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,208,601 | 0 | G | T | 78.4%
| 78.2
/ 29.6
| 51 | T224T (ACG→ACT) | yehL | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (6/5); new base T (40/0); total (46/5) |
| Fisher's exact test for biased strand distribution p-value = 1.97e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 |
CGATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGG > W3110S.gb/2208544‑2208635
|
cgATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt < 1:990157/62‑1 (MQ=255)
cgATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt < 1:2826358/62‑1 (MQ=255)
cgATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt < 1:2680262/62‑1 (MQ=255)
cgATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt < 1:632788/62‑1 (MQ=255)
gATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGttt < 1:1889160/61‑1 (MQ=255)
tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTa > 1:1339242/1‑51 (MQ=255)
tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg > 1:1017350/1‑60 (MQ=255)
tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg > 1:1993570/1‑60 (MQ=255)
tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg > 1:2396507/1‑60 (MQ=255)
tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg > 1:2251130/1‑60 (MQ=255)
tgaGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCg > 1:2339313/1‑60 (MQ=255)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTc > 1:2656996/1‑36 (MQ=255)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTc > 1:985402/1‑36 (MQ=255)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAgct > 1:2397641/1‑45 (MQ=255)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:395810/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2540743/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2905239/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2953371/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:3050393/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:334917/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:367589/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:957454/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:553420/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:684169/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:685455/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:699042/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:752707/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:799556/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:860991/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:924642/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2124127/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1266094/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1545842/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:158365/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1600536/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1768400/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:1796579/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:181571/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2016077/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2041489/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2793098/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2251459/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2287068/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2369525/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2531887/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTgg > 1:2754374/1‑49 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg > 1:2133963/1‑48 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg > 1:461007/1‑48 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTATGGATTTCGCCCAGGAGCTg > 1:1379311/1‑48 (MQ=38)
ttCGATTTTGAAACTGTCTTTCCGATTAAGGATTTCGCCCAGGAGCTgg > 1:2765893/1‑49 (MQ=37)
ttCGATTTTGAAACTGTCTTT‑CGATTATGGATTTCGCCCAGGAGCTgg > 1:2308705/1‑48 (MQ=25)
|
CGATCGCGGCGTCAATGAAATGAGTGCTGCGCTAAAGCGTCGCTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGG > W3110S.gb/2208544‑2208635
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
