Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I2 R2 64 60.0 3197630 92.7% 2964203 60.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 826,223 G→T G207V (GGT→GTT)  ychP → predicted invasin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE826,2230GT100.0% 98.8 / NA 31G207V (GGT→GTT) ychPpredicted invasin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/16);  total (15/16)

ACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGG  >  minE/826156‑826285
                                                                   |                                                              
aCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTttgttg                                                              <  1:2837238/70‑1 (MQ=255)
aCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGa                                                             >  1:247133/1‑71 (MQ=255)
aCAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGa                                                             >  1:250905/1‑71 (MQ=255)
 cAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGAt                                                            <  1:2607736/71‑1 (MQ=255)
  aGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATc                                                           >  1:1536721/1‑71 (MQ=255)
       ggATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGtt                                                      <  1:1122266/71‑1 (MQ=255)
       ggATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGtt                                                      <  1:3125471/71‑1 (MQ=255)
         aTGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGa                                                    >  1:2412881/1‑71 (MQ=255)
             gcATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTttgttg                                                              <  1:1141585/57‑1 (MQ=255)
             gcATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTttgttg                                                              <  1:343728/57‑1 (MQ=255)
             gcATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTttgttg                                                              <  1:1285753/57‑1 (MQ=255)
             gcATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTttgttg                                                              <  1:931392/57‑1 (MQ=255)
             gcATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTttgttg                                                              <  1:117196/57‑1 (MQ=255)
               aTGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGtt                                              >  1:1540205/1‑71 (MQ=255)
                tGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTAATTTGTTGATCGTGTTGATTTGtt                                              >  1:1381468/1‑70 (MQ=255)
                  ccGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTaa                                           <  1:2391089/71‑1 (MQ=255)
                       cTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGAtt                                                  >  1:607339/1‑59 (MQ=255)
                       cTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTg                                      >  1:648684/1‑71 (MQ=255)
                            aaCACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTAc                                  <  1:2444701/70‑1 (MQ=255)
                            aaCACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTAc                                  <  1:1299559/70‑1 (MQ=255)
                            aaCACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACg                                 >  1:2012164/1‑71 (MQ=255)
                                    aaTACCAGTGTCAGCCTAGAACAGTATATTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATc                          >  1:2839069/1‑70 (MQ=255)
                                         cAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTAt                           <  1:2240252/64‑1 (MQ=255)
                                         cAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAAt                     >  1:1575717/1‑70 (MQ=255)
                                         cAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATc                    >  1:999003/1‑71 (MQ=255)
                                         cAGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATc                    <  1:1951119/71‑1 (MQ=255)
                                          aGTGTCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATcc                   <  1:752278/71‑1 (MQ=255)
                                            tgtCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCg                 <  1:584779/71‑1 (MQ=255)
                                            tgtCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCg                 <  1:77902/71‑1 (MQ=255)
                                             gtCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGt                >  1:1181149/1‑71 (MQ=255)
                                              tCAGCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGt                >  1:2241652/1‑70 (MQ=255)
                                                 gCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTAt                           <  1:2020660/56‑1 (MQ=255)
                                                 gCCTAGAACAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTcgcg            <  1:3109248/71‑1 (MQ=255)
                                                   cTAGAACAGTATTTTGTTGATCGTGTTGATTTGttt                                             <  1:908656/36‑1 (MQ=255)
                                                         cAGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTAc                                  <  1:325960/41‑1 (MQ=255)
                                                          aGTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTg   <  1:2500726/71‑1 (MQ=255)
                                                           gTATTTTGTTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTgg  >  1:1383295/1‑71 (MQ=255)
                                                                   |                                                              
ACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGG  >  minE/826156‑826285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A