Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,207,332 |
A→G |
intergenic (‑22/+408) |
tldD ← / ← yhdP |
predicted peptidase/conserved membrane protein, predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,207,332 | 0 | A | G | 100.0%
| 121.3
/ NA
| 34 | intergenic (‑22/+408) | tldD/yhdP | predicted peptidase/conserved membrane protein, predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/9); total (25/9) |
CGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATG > minE/2207268‑2207398
|
cGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCAACTCGTTAGTTGCTGCAg > 1:668436/1‑71 (MQ=255)
ttCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTa < 1:465518/71‑1 (MQ=255)
cTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTagag < 1:390756/67‑1 (MQ=255)
gCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGAttt < 1:2936156/69‑1 (MQ=255)
ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTa < 1:547062/57‑1 (MQ=255)
ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTAc < 1:1145056/70‑1 (MQ=255)
cACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTg < 1:1607201/70‑1 (MQ=255)
aCTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTa > 1:2600937/1‑51 (MQ=255)
aCTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTa > 1:2001808/1‑51 (MQ=255)
aCCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGAtttttt > 1:1698837/1‑58 (MQ=255)
aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAg > 1:2538292/1‑42 (MQ=255)
aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAg > 1:1894497/1‑42 (MQ=255)
aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAg > 1:1244683/1‑42 (MQ=255)
aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAg > 1:1782030/1‑42 (MQ=255)
tAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTAc > 1:1006744/1‑53 (MQ=255)
tAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTAc > 1:1379811/1‑53 (MQ=255)
aaGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGt > 1:1203362/1‑70 (MQ=255)
aaGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGt > 1:2504221/1‑70 (MQ=255)
gACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGaa > 1:1866829/1‑71 (MQ=255)
gACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGaa > 1:1098226/1‑71 (MQ=255)
gACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGaa > 1:2486903/1‑71 (MQ=255)
gACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGaa > 1:2490651/1‑71 (MQ=255)
aCTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAAc < 1:565998/71‑1 (MQ=255)
aCTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAAc < 1:1017656/71‑1 (MQ=255)
cATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGcc > 1:1058735/1‑71 (MQ=255)
aTCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGccc > 1:1729044/1‑71 (MQ=255)
cGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCt > 1:1098283/1‑70 (MQ=255)
cGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTa > 1:1508665/1‑71 (MQ=255)
cGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTa > 1:1435416/1‑71 (MQ=255)
tttGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACgg > 1:545339/1‑41 (MQ=255)
aCTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTAc > 1:3079346/1‑71 (MQ=255)
cTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTa < 1:74378/69‑1 (MQ=255)
cTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACg > 1:2188330/1‑71 (MQ=255)
cGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGt > 1:132840/1‑71 (MQ=255)
agttgCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATg > 1:2008048/1‑71 (MQ=255)
|
CGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATG > minE/2207268‑2207398
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
