Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R2 57 53.4 717274 8.4% 60251 54.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,192,489 A→C F586V (TTT→GTT)  yehB ← predicted outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,192,4890AC100.0% 301.9 / NA 115F586V (TTT→GTT) yehBpredicted outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/115);  total (0/115)

Only 100 of 115 total aligned reads displayed.

ATTTGAGGCAAACCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCCGACGA  >  W3110S.gb/2192478‑2192538
           |                                                 
ttctGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:541635/58‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:484212/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:562158/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:556331/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:548781/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:533268/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:525568/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:522323/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:521464/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:502128/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:496416/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:489153/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:488852/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:564599/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:468808/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:464410/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:452087/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:450328/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:446695/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:439988/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:436002/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:406062/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:402369/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:392843/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:381411/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:662137/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:85773/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:85144/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:84018/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:828/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:79962/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:7939/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:7429/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:696938/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:693120/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:692979/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:688517/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:665655/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:380180/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:656233/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:649210/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:634553/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:627139/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:621298/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:61906/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:61805/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:60572/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:604597/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:599002/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:595835/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:150800/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:1002/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:237488/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:23089/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:228796/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:217331/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:195758/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:184724/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:18411/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:167102/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:16469/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:161613/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:156459/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:246418/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:140007/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:136946/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:134020/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:131871/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:123051/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:122876/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:119859/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:117530/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:117006/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:11325/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:105155/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:315708/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:377188/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:369362/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:365113/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:351705/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:351393/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:338431/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:332442/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:322462/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:318017/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:316465/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:249662/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:305995/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:305930/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:302652/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:29679/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:29452/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:287734/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:287341/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:273918/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:273664/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:273567/54‑1 (MQ=255)
       gCAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:270342/54‑1 (MQ=255)
        cAACCCTCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:338925/53‑1 (MQ=255)
        cAACCCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCcgacga  <  1:11235/53‑1 (MQ=255)
           |                                                 
ATTTGAGGCAAACCCCTGATCATCAAACGTCGTTGAGTTAGACATATATATTTGCCGACGA  >  W3110S.gb/2192478‑2192538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A