Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F28 I3 R2
|
52 |
92.4 |
4932871 |
87.1% |
4296530 |
63.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,889,046 |
G→A |
P395S (CCC→TCC) |
gudX ← |
predicted glucarate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,889,046 | 0 | G | A | 85.7%
| 233.4
/ 28.1
| 91 | P395S (CCC→TCC) | gudX | predicted glucarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (13/0); new base A (27/51); total (40/51) |
| Fisher's exact test for biased strand distribution p-value = 6.28e-06 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 |
TTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCC > minE/1888987‑1889113
|
tttATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGaa > 1:1913389/1‑71 (MQ=255)
tttATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGaa > 1:652130/1‑71 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3803117/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:4158740/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3341124/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3283461/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:4027301/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3839268/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:1031946/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:1344005/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3612081/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:1266762/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:760144/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:3607541/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCAt > 1:868296/1‑63 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:2689616/1‑71 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:2098791/1‑71 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:483239/1‑71 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:1592141/1‑71 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACg > 1:533497/1‑71 (MQ=255)
tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGCACGCAACAgg > 1:1417736/1‑67 (MQ=255)
aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGACCGGCAAttttt > 1:1293256/1‑70 (MQ=255)
aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTc > 1:3912591/1‑71 (MQ=255)
aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTc > 1:619771/1‑71 (MQ=255)
tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:412012/70‑1 (MQ=255)
tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:356644/70‑1 (MQ=255)
tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:2820897/70‑1 (MQ=255)
tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:3202700/70‑1 (MQ=255)
tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCg < 1:1087348/70‑1 (MQ=255)
ttGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTaa > 1:3954479/1‑71 (MQ=255)
cagtccagtTCCATGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:3951951/1‑71 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCg > 1:4362958/1‑67 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:2818919/1‑71 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt < 1:2899785/71‑1 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:3566956/1‑71 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:4746510/1‑71 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:32672/1‑71 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:1139762/1‑71 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtt > 1:845749/1‑71 (MQ=255)
cagtccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAG‑GGAttt > 1:3225839/1‑71 (MQ=255)
tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:3190011/71‑1 (MQ=255)
tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:805980/71‑1 (MQ=255)
tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:2171256/71‑1 (MQ=255)
tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:919376/71‑1 (MQ=255)
tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:2946236/71‑1 (MQ=255)
tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:1640246/71‑1 (MQ=255)
tccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:1653265/71‑1 (MQ=255)
ccagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:3773811/70‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCCGCGGATTTTggg > 1:4256665/1‑71 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:4716013/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:4437802/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:4431958/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:954317/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:1790154/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:1319566/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:1202272/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:3151425/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:3274238/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:89441/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:2974499/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:806512/68‑1 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTggg > 1:2024135/1‑71 (MQ=255)
cagtTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTggg > 1:3256489/1‑71 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAtttt < 1:2275652/65‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:3046498/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:4651277/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:4594402/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:524919/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:1778374/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:247298/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:2409934/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:686953/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:3754457/71‑1 (MQ=255)
ttCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:2356485/71‑1 (MQ=255)
tCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:3475880/65‑1 (MQ=255)
tCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCa < 1:4404684/70‑1 (MQ=255)
ccACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAgg > 1:3053284/1‑71 (MQ=255)
ccACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAgg > 1:1480595/1‑71 (MQ=255)
ccACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAgg > 1:3066442/1‑71 (MQ=255)
cACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGc < 1:4637685/71‑1 (MQ=255)
cACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGc < 1:4037983/71‑1 (MQ=255)
gCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:3599683/60‑1 (MQ=255)
ccGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCT‑CAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:1832078/71‑1 (MQ=255)
cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:969392/71‑1 (MQ=255)
cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:3271558/71‑1 (MQ=255)
cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:3155874/71‑1 (MQ=255)
cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:2610174/71‑1 (MQ=255)
cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:2400201/71‑1 (MQ=255)
cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:1821701/71‑1 (MQ=255)
cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:1497951/71‑1 (MQ=255)
cGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:1461261/71‑1 (MQ=255)
|
TTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCC > minE/1888987‑1889113
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
