Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I3 R2 52 92.4 4932871 87.1% 4296530 63.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,924,650 C→T H339Y (CAT→TAT)  galR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,924,6500CT100.0% 370.9 / NA 112H339Y (CAT→TAT) galRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/61);  total (51/61)

Only 100 of 113 total aligned reads displayed.

TCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGC  >  minE/1924595‑1924714
                                                       |                                                                
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:2940111/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:4023278/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:2134317/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:4724417/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:3150083/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:714036/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:4832028/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:3264876/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:2378059/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:1060426/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:964587/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:1417900/59‑1 (MQ=255)
tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:4879867/59‑1 (MQ=255)
  cGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATg                                                               <  1:4758567/57‑1 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:3763378/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:2360221/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:3878298/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:3082833/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:2247962/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:2129366/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:695956/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:2044809/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:4610147/1‑70 (MQ=255)
      gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCg                                              >  1:1736670/1‑70 (MQ=255)
            aCGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTa                                        >  1:628911/1‑70 (MQ=255)
            aCGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTa                                        >  1:3656908/1‑70 (MQ=255)
            aCGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTa                                        >  1:4288849/1‑70 (MQ=255)
             cgtcgtCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTa                                        >  1:2925202/1‑69 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:3113278/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:3116232/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:3217032/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:3239062/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:2569574/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:2953799/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:4523441/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:3745052/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:2794853/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:966828/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:532591/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:910854/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:2409783/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:2937862/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:604374/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGc                                             <  1:1924688/62‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGc                                    <  1:2348963/71‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGc                                    <  1:908024/71‑1 (MQ=255)
               tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATG‑AACCAGCGACTAACCGc                                             <  1:4019186/61‑1 (MQ=255)
                 gtcATTCAGTTTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:2292919/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCac                                                            >  1:3196809/1‑44 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCa                                                             >  1:4900338/1‑44 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:4272402/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:982713/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:4304901/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:4215419/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:4572737/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:4591117/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:536523/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:2382263/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:680511/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAc                                                           >  1:1999379/1‑46 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:4701027/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:4872562/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:571275/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:3379277/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:2250212/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:2502110/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:1740103/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:3262943/1‑62 (MQ=255)
                 gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAg                                           >  1:3411247/1‑62 (MQ=255)
                    aTTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTc                               <  1:4240851/71‑1 (MQ=255)
                    aTTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTc                               <  1:3586406/71‑1 (MQ=255)
                                       gCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttcttc             <  1:4190744/70‑1 (MQ=255)
                                       gCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttcttc             <  1:489935/70‑1 (MQ=255)
                                       gCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttcttc             <  1:2831038/70‑1 (MQ=255)
                                           gAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGAt          <  1:902086/69‑1 (MQ=255)
                                           gAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGAt          <  1:2266006/69‑1 (MQ=255)
                                                aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAgc                           >  1:2094530/1‑47 (MQ=255)
                                                aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg   >  1:936628/1‑71 (MQ=255)
                                                aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg   >  1:3351614/1‑71 (MQ=255)
                                                aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg   >  1:3467959/1‑71 (MQ=255)
                                                aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg   >  1:1622219/1‑71 (MQ=255)
                                                aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg   >  1:3489519/1‑71 (MQ=255)
                                                aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg   >  1:2692871/1‑71 (MQ=255)
                                                aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg   >  1:1405020/1‑71 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTaa                   <  1:4483486/54‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTaa                   <  1:2115981/54‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc  <  1:1448040/71‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc  <  1:2233052/71‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc  <  1:4172633/71‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc  <  1:2646025/71‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc  <  1:493269/71‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc  <  1:2819029/71‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc  <  1:3442240/71‑1 (MQ=255)
                                                 aGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAAATCCAGCGCCAGTaa                   <  1:2958273/54‑1 (MQ=255)
                                                  gTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATg         <  1:2492728/63‑1 (MQ=255)
                                                  gTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATg         <  1:2983741/63‑1 (MQ=255)
                                                  gTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATg         <  1:3103591/63‑1 (MQ=255)
                                                  gTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATg         <  1:4500190/63‑1 (MQ=255)
                                                  gTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATg         <  1:4121005/63‑1 (MQ=255)
                                                  gTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATg         <  1:1078746/63‑1 (MQ=255)
                                                       |                                                                
TCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGC  >  minE/1924595‑1924714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A